ID   PB2_I02A2               Reviewed;         753 AA.
AC   Q6DNM1;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 2.
DT   23-FEB-2022, entry version 67.
DE   RecName: Full=Polymerase basic protein 2 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   Flags: Fragment;
GN   Name=PB2 {ECO:0000255|HAMAP-Rule:MF_04062};
OS   Influenza A virus (strain A/Chicken/Hong Kong/31.2/2002 H5N1 genotype X1).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=284169;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15241415; DOI=10.1038/nature02746;
RA   Li K.S., Guan Y., Wang J., Smith G.J.D., Xu K.M., Duan L., Rahardjo A.P.,
RA   Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T.S.,
RA   Chaisingh A., Auewarakul P., Long H.T., Hanh N.T.H., Webby R.J.,
RA   Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G.,
RA   Peiris J.S.M.;
RT   "Genesis of a highly pathogenic and potentially pandemic H5N1 influenza
RT   virus in eastern Asia.";
RL   Nature 430:209-213(2004).
RN   [2]
RP   SEQUENCE REVISION.
RA   Li K.S., Guan Y., Wang J., Smith G.J.D., Xu K.M., Duan L., Rahardjo A.P.,
RA   Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T.S.,
RA   Chaisingh A., Auewarakul P., Long H.T., Hanh N.T.H., Lim W., Webby R.J.,
RA   Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G.,
RA   Peiris J.S.M.;
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Recognizes and binds the 7-
CC       methylguanosine-containing cap of the target pre-RNA which is
CC       subsequently cleaved after 10-13 nucleotides by the viral protein PA.
CC       Plays a role in the initiation of the viral genome replication and
CC       modulates the activity of the ribonucleoprotein (RNP) complex.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). {ECO:0000255|HAMAP-
CC       Rule:MF_04062}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04062}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAT73562.2; Type=Frameshift;
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DR   EMBL; AY651731; AAT73562.2; ALT_FRAME; Genomic_RNA.
DR   SMR; Q6DNM1; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.30.90; -; 1.
DR   HAMAP; MF_04062; INV_PB2; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   InterPro; IPR037258; PDB2_C.
DR   Pfam; PF00604; Flu_PB2; 1.
DR   SUPFAM; SSF160453; SSF160453; 1.
PE   3: Inferred from homology;
KW   Cap snatching; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host nucleus;
KW   Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW   mRNA capping; mRNA processing; Viral transcription; Virion.
FT   CHAIN           <1..753
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000311149"
FT   MOTIF           730..733
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04062"
FT   SITE            621
FT                   /note="Avian adaptation"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04062"
FT   NON_TER         1
SQ   SEQUENCE   753 AA;  85017 MW;  89A110D4C5ADE464 CRC64;
     LRDLMSQSRT REILTKTTVD HVAIIKKYTS GRQEKNPALR MKWMMAMKYP ITADKRIMGM
     IPERNEQGQT LWSKTNDAGS DRVMVSPLAV TWWNRNGPTT STVHYPKVYK TYFEKVERLK
     HGTFGPVHFR NQVKIRRRVD INPGHADLSA KEAQDVIMEV VFPNEVGARI LTSESQLTIT
     KEKKEELQDC KIAPLMVAYM LERELVRKTR FLPVAGGTSS VYIEVLHLTQ GTCWEQMYTP
     GGEVRNDDVD QSLIIAARNI VRRATVSADP LASLLEMCHS TQIGGIRMVD ILRQNPTEEQ
     AVDICKAAMG LRISSSFSFG GFTFKRTSGS SVKREEEVLT GNLQTLKIRV HEGYEEFTMV
     GRRATAILRK ATRRLIQLIV SGRDEQSIAE AIIVAMVFSQ EDCMIKAVRG DLNFVNRANQ
     RLNPMHQLLR HFQKDAKVLF QNWGIEPIDN VMGMIGILPD MTPSTEMSLR GVRVSKMGVD
     EYSSTERVVV SIDRFLRVRD QRGNVLLSPE EVSETQGTEK LTITYSSSMM WEINGPESVL
     VNTYQWIIRN WETVKIQWSQ DPTMLYNKME FEPFQSLVPK AARGQYSGFV RTLFQQMRDV
     LGTFDTVQII KLLPFAAAPP EQSRMQFSSL TVNVRGSGMR ILVRGNSPVF NYNKATKRLT
     VLGKDAGALT EDPDEGTAGV ESAVLRGFLI LGKEDKRYGP ALSINELSNL AKGEKANVLI
     GQGDVVLVMK RKRDSSILTD SQTATKRIRM AIN