ID   PB2_I02A4               Reviewed;         757 AA.
AC   Q6DNL9;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   23-FEB-2022, entry version 66.
DE   RecName: Full=Polymerase basic protein 2 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   Flags: Fragment;
GN   Name=PB2 {ECO:0000255|HAMAP-Rule:MF_04062};
OS   Influenza A virus (strain A/Silky Chicken/Hong Kong/YU100/2002 H5N1
OS   genotype X3).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=284214;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15241415; DOI=10.1038/nature02746;
RA   Li K.S., Guan Y., Wang J., Smith G.J.D., Xu K.M., Duan L., Rahardjo A.P.,
RA   Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T.S.,
RA   Chaisingh A., Auewarakul P., Long H.T., Hanh N.T.H., Webby R.J.,
RA   Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G.,
RA   Peiris J.S.M.;
RT   "Genesis of a highly pathogenic and potentially pandemic H5N1 influenza
RT   virus in eastern Asia.";
RL   Nature 430:209-213(2004).
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Recognizes and binds the 7-
CC       methylguanosine-containing cap of the target pre-RNA which is
CC       subsequently cleaved after 10-13 nucleotides by the viral protein PA.
CC       Plays a role in the initiation of the viral genome replication and
CC       modulates the activity of the ribonucleoprotein (RNP) complex.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). {ECO:0000255|HAMAP-
CC       Rule:MF_04062}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04062}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
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DR   EMBL; AY651733; AAT73564.1; -; Genomic_RNA.
DR   SMR; Q6DNL9; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.30.90; -; 1.
DR   HAMAP; MF_04062; INV_PB2; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   InterPro; IPR037258; PDB2_C.
DR   Pfam; PF00604; Flu_PB2; 1.
DR   SUPFAM; SSF160453; SSF160453; 1.
PE   3: Inferred from homology;
KW   Cap snatching; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host nucleus;
KW   Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW   mRNA capping; mRNA processing; Viral transcription; Virion.
FT   CHAIN           <1..>757
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000311151"
FT   MOTIF           735..738
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04062"
FT   SITE            626
FT                   /note="Avian adaptation"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04062"
FT   NON_TER         1
FT   NON_TER         757
SQ   SEQUENCE   757 AA;  85587 MW;  5E29A03A836B0C86 CRC64;
     ERIKELRDLM SQSRTREILT KTTVDHMAII KKYTSGRQEK NPALRMKWMM AMKYPITADK
     RIIEMIPERN EQGQTLWSKT NDAGSDRVMV SPLAVTWWNR NGPTTSAVHY PKVYKTYFEK
     VERLKHGTFG PVHFRNQVKI RRRVDINPGH ADLSAKEAQD VIMEVVFPNE VGARILTSES
     QLTITKEKKE ELQDCKIAPL MVAYMLEREL VRKTRFLPVA GGTSSVYIEV LHLTQGTCWE
     QMYTPGGEVR NDDVDQSLII AARNIVRRAT VSADPLASLL EMCHSTQIGG IRMVDILRQN
     PTEEQAVDIC KAAMGLRISS SFSFGGFTFK RTSGSSVKKE EEVLTGNLQT LKIRVHEGYE
     EFTMVGRRAT AILRKATRRL IQLIVSGRDE QSIAEAIIVA MVFSQEDCMI KAVRGDLNFV
     NRANQRLNPM HQLLRHFQKD AKVLFQNWGI EPIDNVMGMI GILPDMTPST EMSLRGVRVS
     KMGVDEYSST ERVVVSIDRF LRVRDQRGNV LLSPEEVSET QGTEKLTITY SSSMMWEING
     PESVLVNTYQ WIIRNWETVK IQWSQDPTML YNKMEFEPFQ SLVPKAARGQ YSGFVRTLFQ
     QMRDVLGTFD TVQIIKLLPF AAAPPEQSRM QFSSLTVNVR GSGMRILVRG NSPVFNYNKA
     TKRLTVLGKD AGALTEDPDE GTAGVESAVL RGFLILGKED KRYGPALSIN ELSNLAKGEK
     ANVLIGQGDV VLVMKRKRDS SILTDSQTAT KRIRMAI