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PB2_INBLE
ID   PB2_INBLE               Reviewed;         770 AA.
AC   Q9QLL6;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Polymerase basic protein 2 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000255|HAMAP-Rule:MF_04062};
GN   Name=PB2 {ECO:0000255|HAMAP-Rule:MF_04062};
OS   Influenza B virus (strain B/Lee/1940).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Betainfluenzavirus.
OX   NCBI_TaxID=518987;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10725418; DOI=10.1099/0022-1317-81-4-929;
RA   Hiromoto Y., Saito T., Lindstrom S.E., Li Y., Nerome R., Sugita S.,
RA   Shinjoh M., Nerome K.;
RT   "Phylogenetic analysis of the three polymerase genes (PB1, PB2 and PA) of
RT   influenza B virus.";
RL   J. Gen. Virol. 81:929-937(2000).
RN   [2]
RP   FUNCTION, AND INTERACTION WITH HOST ANP32A AND ANP32B.
RC   STRAIN=B/Yamagata/PJ/2018;
RX   PubMed=33045004; DOI=10.1371/journal.ppat.1008989;
RA   Zhang Z., Zhang H., Xu L., Guo X., Wang W., Ji Y., Lin C., Wang Y.,
RA   Wang X.;
RT   "Selective usage of ANP32 proteins by influenza B virus polymerase:
RT   Implications in determination of host range.";
RL   PLoS Pathog. 16:e1008989-e1008989(2020).
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Recognizes and binds a wide
CC       range of cap structures of target pre-RNAs which are subsequently
CC       cleaved after 10-13 nucleotides by the viral protein PA. Plays a role
CC       in the initiation of the viral genome replication and modulates the
CC       activity of the ribonucleoprotein (RNP) complex. {ECO:0000255|HAMAP-
CC       Rule:MF_04062, ECO:0000269|PubMed:33045004}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). Interacts with host
CC       ANP32A (via C-terminus) and ANP32B; these interactions promote viral
CC       RNA synthesis (PubMed:33045004). {ECO:0000255|HAMAP-Rule:MF_04062,
CC       ECO:0000269|PubMed:33045004}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04062}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
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DR   EMBL; AF101982; AAF06851.1; -; Genomic_RNA.
DR   RefSeq; NP_056658.1; NC_002205.1.
DR   PDB; 5EF9; X-ray; 1.70 A; A=320-485.
DR   PDB; 5EFA; X-ray; 1.90 A; A=320-485.
DR   PDB; 5EFC; X-ray; 1.90 A; A=320-485.
DR   PDB; 6XQA; X-ray; 2.16 A; C/F=550-558.
DR   PDBsum; 5EF9; -.
DR   PDBsum; 5EFA; -.
DR   PDBsum; 5EFC; -.
DR   PDBsum; 6XQA; -.
DR   SMR; Q9QLL6; -.
DR   PRIDE; Q9QLL6; -.
DR   GeneID; 956546; -.
DR   KEGG; vg:956546; -.
DR   Proteomes; UP000008158; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.30.90; -; 1.
DR   HAMAP; MF_04062; INV_PB2; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   InterPro; IPR037258; PDB2_C.
DR   Pfam; PF00604; Flu_PB2; 1.
DR   SUPFAM; SSF160453; SSF160453; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cap snatching;
KW   Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host nucleus;
KW   Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW   mRNA capping; mRNA processing; Reference proteome; Viral transcription;
KW   Virion.
FT   CHAIN           1..770
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000391500"
FT   MOTIF           740..743
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04062"
FT   STRAND          325..327
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          330..337
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          340..347
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          353..361
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          363..369
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          372..379
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          382..389
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   HELIX           393..406
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   HELIX           410..414
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          427..429
FT                   /evidence="ECO:0007829|PDB:5EFC"
FT   HELIX           432..441
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   HELIX           444..451
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          453..455
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          462..465
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          471..476
FT                   /evidence="ECO:0007829|PDB:5EF9"
FT   STRAND          479..482
FT                   /evidence="ECO:0007829|PDB:5EF9"
SQ   SEQUENCE   770 AA;  88025 MW;  F399A58CCD924CD7 CRC64;
     MTLAKIELLK QLLRDNEAKT VLRQTTVDQY NIIRKFNTSR IEKNPSLRMK WAMCSNFPLA
     LTKGDMANRI PLEYKGIQLK TNAEDIGTKG QMCSIAAVTW WNTYGPIGDT EGFEKVYESF
     FLRKMRLDNA TWGRITFGPV ERVRKRVLLN PLTKEMPPDE ASNVIMEILF PKEAGIPRES
     TWIHRELIKE KREKLKGTMI TPIVLAYMLE RELVARRRFL PVAGATSAEF IEMLHCLQGE
     NWRQIYHPGG NKLTESRSQS MIVACRKIIR RSIVASNPLE LAVEIANKTV IDTEPLKSCL
     AALDGGDVAC DIIRAALGLK IRQRQRFGRL ELKRISGRGF KNDEEILIGN GTIQKIGIWD
     GEEEFHVRCG ECRGILKKSQ MRMEKLLINS AKKEDMKDLI ILCMVFSQDT RMFQGVRGEI
     NFLNRAGQLL SPMYQLQRYF LNRSNDLFDQ WGYEESPKAS ELHGINELMN ASDYTLKGVV
     VTKNVIDDFS STETEKVSIT KNLSLIKRTG EVIMGANDVS ELESQAQLMI TYDTPKMWEM
     GTTKELVQNT YQWVLKNLVT LKAQFLLGKE DMFQWDAFEA FESIIPQKMA GQYSGFARAV
     LKQMRDQEVM KTDQFIKLLP FCFSPPKLRS NGEPYQFLRL MLKGGGENFI EVRKGSPLFS
     YNPQTEILTI CGRMMSLKGK IEDEERNRSM GNAVLAGFLV SGKYDPDLGD FKTIEELERL
     KPGEKANILL YQGKPVKVVK RKRYSALSND ISQGIKRQRM TVESMGWALS
 
 
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