ID   PB2_INCAA               Reviewed;         774 AA.
AC   Q6I7C4; Q82668;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Polymerase basic protein 2 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000255|HAMAP-Rule:MF_04062};
GN   Name=PB2 {ECO:0000255|HAMAP-Rule:MF_04062};
OS   Influenza C virus (strain C/Ann Arbor/1/1950).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX   NCBI_TaxID=11553;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8607283; DOI=10.1016/s0168-1702(95)00077-1;
RA   Lapatschek M.S., Marschall M., Meier-Ewert H.;
RT   "The persistent variant of influenza C virus carries one characteristic
RT   point mutation in RNA segment 1.";
RL   Virus Res. 39:47-54(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15218173; DOI=10.1099/vir.0.79937-0;
RA   Muraki Y., Washioka H., Sugawara K., Matsuzaki Y., Takashita E., Hongo S.;
RT   "Identification of an amino acid residue on influenza C virus M1 protein
RT   responsible for formation of the cord-like structures of the virus.";
RL   J. Gen. Virol. 85:1885-1893(2004).
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Recognizes and binds a wide
CC       range of cap structures of target pre-RNAs which are subsequently
CC       cleaved after 10-13 nucleotides by the viral protein PA. Plays a role
CC       in the initiation of the viral genome replication and modulates the
CC       activity of the ribonucleoprotein (RNP) complex. {ECO:0000255|HAMAP-
CC       Rule:MF_04062}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). {ECO:0000255|HAMAP-
CC       Rule:MF_04062}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04062}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
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DR   EMBL; U20228; AAB01664.1; -; Genomic_RNA.
DR   EMBL; AB126191; BAD24937.1; -; Genomic_RNA.
DR   RefSeq; YP_089652.1; NC_006307.1.
DR   SMR; Q6I7C4; -.
DR   PRIDE; Q6I7C4; -.
DR   DNASU; 3077363; -.
DR   GeneID; 3077363; -.
DR   KEGG; vg:3077363; -.
DR   Proteomes; UP000008286; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   HAMAP; MF_04062; INV_PB2; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   Pfam; PF00604; Flu_PB2; 1.
PE   3: Inferred from homology;
KW   Cap snatching; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host nucleus;
KW   Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW   mRNA capping; mRNA processing; Reference proteome; Viral transcription;
KW   Virion.
FT   CHAIN           1..774
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000269454"
FT   CONFLICT        266
FT                   /note="M -> I (in Ref. 1; AAB01664)"
FT   CONFLICT        330
FT                   /note="P -> Q (in Ref. 1; AAB01664)"
SQ   SEQUENCE   774 AA;  87843 MW;  B8898C2847473DD4 CRC64;
     MSFLLTIAKE YKRLCQDAKA AQMMTVGTVS NYTTFKKWTT SRKEKNPSLR MRWAMSSKFP
     IIANKRMLEE AQIPKEHNNV ALWEDTEDVS KRDHVLASAS CINYWNFCGP CVNNSEVIKE
     VYKSRFGRLE RRKEIMWKEL RFTLVDRQRR RVDTQPVEQR LRTGEIKDLQ MWTLFEDEAP
     LASKFILDNY GLVKEMRSKF ANKPLNKEVV AHMLEKQFNP ESRFLPVFGA IRPERMELIH
     ALGGETWIQE ANTAGISNVD QRKNDMRAVC RKVCLAANAS IMNAKSKLVE YIKSTSMRIG
     ETERKLEELI LETDDVSPEV TLCKSALGGP LGKTLSFGPM LLKKISGSGV KVKDTVYIQG
     VRAVQFEYWS EQEEFYGEYK SATALFSRKE RSLEWITIGG GINEDRKRLL AMCMIFCRDG
     DYFKDAPATI TMADLSTKLG REIPYQYVMM NWIQKSEDNL EALLYSRGIV ETNPGKMGSS
     MGIDGSKRAI KSLRAVTIQS GKIDMPESKE KIHLELSDNL EAFDSSGRIV ATILDLPSDK
     KVTFQDVSFQ HPDLAVLRDE KTAITKGYEA LIKRLGTGDN DIPSLIAKKD YLSLYNLPEV
     KLMAPLIRPN RKGVYSRVAR KLVSTQVTTG HYSLHELIKV LPFTYFAPKQ GMFEGRLFFS
     NDSFVEPGVN NNVFSWSKAD SSKIYCHGIA IRVPLVVGDE HMDTSLALLE GFSVCENDPR
     APMVTRQDLI DVGFGQKVRL FVGQGSVRTF KRTASQRAAS SDVNKNVKKI KMSN