ID   PB2_INCBE               Reviewed;         774 AA.
AC   P21770;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Polymerase basic protein 2 {ECO:0000255|HAMAP-Rule:MF_04062};
DE   AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000255|HAMAP-Rule:MF_04062};
GN   Name=PB2 {ECO:0000255|HAMAP-Rule:MF_04062};
OS   Influenza C virus (strain C/Berlin/1/1985).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX   NCBI_TaxID=11554;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2263472; DOI=10.1093/nar/18.23.7133;
RA   Stompor S., Santibanez Koref M.;
RT   "Sequence of the PB2 homolog of influenza C/Berlin/1/85.";
RL   Nucleic Acids Res. 18:7133-7133(1990).
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Recognizes and binds a wide
CC       range of cap structures of target pre-RNAs which are subsequently
CC       cleaved after 10-13 nucleotides by the viral protein PA. Plays a role
CC       in the initiation of the viral genome replication and modulates the
CC       activity of the ribonucleoprotein (RNP) complex. {ECO:0000255|HAMAP-
CC       Rule:MF_04062}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). {ECO:0000255|HAMAP-
CC       Rule:MF_04062}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04062}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04062}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04062}.
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DR   EMBL; X55992; CAA39464.1; -; mRNA.
DR   SMR; P21770; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   HAMAP; MF_04062; INV_PB2; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   Pfam; PF00604; Flu_PB2; 1.
PE   2: Evidence at transcript level;
KW   Cap snatching; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host nucleus;
KW   Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW   mRNA capping; mRNA processing; Viral transcription; Virion.
FT   CHAIN           1..774
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000078847"
SQ   SEQUENCE   774 AA;  87708 MW;  BA084DE5AEC7D789 CRC64;
     MSLLLTIAKE YKRLCQDAKA AQMMTVGTVS NYTTFKKWTT SRKEKNPSLR MRWAMSSKFP
     IIANKRMLEE AQIPKEHNNV ALWEDTEDVS KRDHVLASAS CINYWNFCGP CVNNSEVIKE
     VYKSRFGRLE RRKEIMWKEL RFTLVDRQRR RVDTQPVEQR LRTGEIKDLQ MWTLFEDEAP
     LASKFILDNY GLVKEMRSKF ANKPLNKEVV AHMLEKQFNP ESRFLPVFGA IRPERMELIH
     ALGGETWIQE ANTAGISNVD QRKNDMRAVC RKVCLAANAS IMNAKSKLVE YIKSTSMRIG
     ETERKLEELI PETDDVSPEV TLCKSALGGP LGKTLSFGPM LLKKISGSGV KVKDTVYIQG
     VRAVQFEYWS EQEEFYGEYK SATALFSRKE RSLEWITIGG GINEDRKRLL AMCMIFCRDG
     DYFKDAPATI TMADLTTKLG REIPYQYVMM NWIQKSEDNL EALLYSRGIV ETNPGKMGSS
     MGIDGSKRAI KSLRAVTIQS GKIDMPESKE KIHLELSDNL EAFDSSGRIV ATILDLPSDK
     KVTFQDVSFQ HPDLAVLRDE KTAITKGYEA LIKRLGTGDN DIPSLIAKKD YLSLYNLPEV
     KLMAPLIRPN RKGVYSRVAR KLVSTQVTTG HYSLHELIKV LPFTYFAPKQ GMFEGRFFFS
     NDSFVEPGVN NNVLSWSKAD SSKIYCHGIA IRVPLVVGDE HMDTSLALLE GFSVCENDPR
     APMVTGQDLI DVGFGQKVRL FVGQGSVRTF KRTASQRAAS SDVNKNVKKI KMSN