PB2_INCJH
ID PB2_INCJH Reviewed; 774 AA.
AC Q9IMP3;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Polymerase basic protein 2 {ECO:0000255|HAMAP-Rule:MF_04062};
DE AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000255|HAMAP-Rule:MF_04062};
GN Name=PB2 {ECO:0000255|HAMAP-Rule:MF_04062};
OS Influenza C virus (strain C/Johannesburg/1/1966).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX NCBI_TaxID=100673;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA Crescenzo-Chaigne B.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2-774.
RX PubMed=10600605; DOI=10.1006/viro.1999.0059;
RA Crescenzo-Chaigne B., Naffakh N., van der Werf S.;
RT "Comparative analysis of the ability of the polymerase complexes of
RT influenza viruses type A, B and C to assemble into functional RNPs that
RT allow expression and replication of heterotypic model RNA templates in
RT vivo.";
RL Virology 265:342-353(1999).
RN [3]
RP FUNCTION, AND INTERACTION WITH HOST ANP32A.
RX PubMed=33208942; DOI=10.1038/s41586-020-2927-z;
RA Carrique L., Fan H., Walker A.P., Keown J.R., Sharps J., Staller E.,
RA Barclay W.S., Fodor E., Grimes J.M.;
RT "Host ANP32A mediates the assembly of the influenza virus replicase.";
RL Nature 587:638-643(2020).
CC -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC stealing mechanism, in which cellular capped pre-mRNAs are used to
CC generate primers for viral transcription. Recognizes and binds a wide
CC range of cap structures of target pre-RNAs which are subsequently
CC cleaved after 10-13 nucleotides by the viral protein PA. Plays a role
CC in the initiation of the viral genome replication and modulates the
CC activity of the ribonucleoprotein (RNP) complex. {ECO:0000255|HAMAP-
CC Rule:MF_04062, ECO:0000269|PubMed:33208942}.
CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC Interacts with nucleoprotein NP (via N-terminus). Interacts with host
CC ANP32A (via C-terminus); this interaction promotes viral RNA synthesis.
CC {ECO:0000255|HAMAP-Rule:MF_04062, ECO:0000269|PubMed:33208942}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04062}. Host
CC nucleus {ECO:0000255|HAMAP-Rule:MF_04062}.
CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family.
CC {ECO:0000255|HAMAP-Rule:MF_04062}.
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DR EMBL; AF170576; AAF89739.2; -; Genomic_RNA.
DR PDB; 5D98; X-ray; 3.90 A; C/F=1-774.
DR PDB; 5D9A; X-ray; 4.30 A; C/F/I/L=1-774.
DR PDB; 6F5P; X-ray; 4.14 A; E/F=1-774.
DR PDB; 6XZD; EM; 3.40 A; CP1/FP1=1-774.
DR PDB; 6XZG; EM; 3.80 A; CP1/FP1=1-774.
DR PDB; 6XZP; EM; 3.30 A; CP1/FP1=1-774.
DR PDB; 6XZQ; EM; 3.60 A; C/F=1-774.
DR PDB; 6XZR; EM; 3.30 A; CP1/FP1=1-774.
DR PDB; 6Y0C; EM; 3.20 A; C=1-774.
DR PDBsum; 5D98; -.
DR PDBsum; 5D9A; -.
DR PDBsum; 6F5P; -.
DR PDBsum; 6XZD; -.
DR PDBsum; 6XZG; -.
DR PDBsum; 6XZP; -.
DR PDBsum; 6XZQ; -.
DR PDBsum; 6XZR; -.
DR PDBsum; 6Y0C; -.
DR SMR; Q9IMP3; -.
DR DIP; DIP-61788N; -.
DR IntAct; Q9IMP3; 1.
DR PRIDE; Q9IMP3; -.
DR Proteomes; UP000138885; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule.
DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR HAMAP; MF_04062; INV_PB2; 1.
DR InterPro; IPR001591; INV_PB2.
DR Pfam; PF00604; Flu_PB2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cap snatching;
KW Eukaryotic host gene expression shutoff by virus;
KW Eukaryotic host transcription shutoff by virus;
KW Host gene expression shutoff by virus; Host nucleus;
KW Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW mRNA capping; mRNA processing; Reference proteome; Viral transcription;
KW Virion.
FT CHAIN 1..774
FT /note="Polymerase basic protein 2"
FT /id="PRO_0000269902"
SQ SEQUENCE 774 AA; 87822 MW; 359A585371A0D09B CRC64;
MSLLLTIAKE YKRLCQDAKA AQMMTVGTVS NYTTFKKWTT SRKEKNPSLR MRWAMSSKFP
IIANKRMLEE AQIPKEHNNV ALWEDTEDVS KRDHVLASAS CINYWNFCGP CVNNSEVIKE
VYKSRFGRLE RRKEIMWKEL RFTLVDRQRR RVDTQPVEQR LRTGEIKDLQ MWTLFEDEAP
LASKFILDNY GLVKEMRSKF ANKPLNKEVV AHMLEKQFNP ESRFLPVFGA IRPERMELIH
ALGGETWIQE ANTAGISNVD QRKNDIRAVC RKVCLAANAS IMNAKSKLVE YIKSTSMRIG
ETERKLEELI LETDDVSPEV TLCKSALGGQ LGKTLSFGPM LLKKISGSGV KVKDTVYIQG
VRAVQFEYWS EQEEFYGEYK SATALFSRKE RSLEWITIGG GINEDRKRLL AMCMIFCRDG
DYFKDAPATI TMADLSTKLG REIPYQYVMM NWIQKSEDNL EALLYSRGIV ETNPGKMGSS
MGIDGSKRAI KSLRAVTIQS GKIDMPESKE KIHLELSDNL EAFDSSGRIV ATILDLPSDK
KVTFQDVSFQ HPDLAVLRDE KTAITKGYEA LIKRLGTGDN DIPSLIAKKD YLSLYNLPEV
KLMAPLIRPN RKGVYSRVAR KLVSTQVTTG HYSLHELIKV LPFTYFAPKQ GMFEGRLFFS
NDSFVEPGVN NNVFSWSKAD SSKIYCHGIA IRVPLVVGDE HMDTSLALLE GFSVCENDPR
APMVTRQDLI DVGFGQKVRL FVGQGSVRTF KRTASQRAAS SDVNKNVKKI KMSN
