ID   PB2_QRFVE               Reviewed;         733 AA.
AC   D0UFC7;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   23-FEB-2022, entry version 31.
DE   RecName: Full=Polymerase basic protein 2;
DE            Short=PB2;
GN   Name=PB2; OrderedLocusNames=Segment 2;
OS   Quaranfil virus (isolate QrfV/Tick/Afghanistan/EG_T_377/1968) (QRFV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Quaranjavirus.
OX   NCBI_TaxID=1559362;
OH   NCBI_TaxID=6939; Ixodidae (hardbacked ticks).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=19726499; DOI=10.1128/jvi.00677-09;
RA   Presti R.M., Zhao G., Beatty W.L., Mihindukulasuriya K.A., da Rosa A.P.,
RA   Popov V.L., Tesh R.B., Virgin H.W., Wang D.;
RT   "Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the
RT   family Orthomyxoviridae.";
RL   J. Virol. 83:11599-11606(2009).
CC   -!- FUNCTION: subunit of the RNA-dependent RNA polymerase which is
CC       responsible for replication and transcription of virus RNA segments.
CC       The transcription of viral mRNAs occurs by a unique mechanism called
CC       cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after
CC       10-13 nucleotides by PA. In turn, these short capped RNAs are used as
CC       primers by PB1 for transcription of viral mRNAs. During virus
CC       replication, PB1 initiates RNA synthesis and copy vRNA into
CC       complementary RNA (cRNA) which in turn serves as a template for the
CC       production of more vRNAs. {ECO:0000250|UniProtKB:P03428}.
CC   -!- SUBUNIT: RNA polymerase is composed of three subunits: PA, PB1 and PB2.
CC       {ECO:0000250|UniProtKB:P03431}.
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DR   EMBL; GQ499302; ACY56278.1; -; Genomic_RNA.
DR   Proteomes; UP000029941; Genome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR001591; INV_PB2.
DR   Pfam; PF00604; Flu_PB2; 1.
PE   3: Inferred from homology;
KW   Cap snatching; mRNA capping; mRNA processing; Reference proteome.
FT   CHAIN           1..733
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000443069"
SQ   SEQUENCE   733 AA;  84784 MW;  51AF691FB9C22F14 CRC64;
     MAATPESLQA RKTRLLSVVR KIKSVALDPL ADQVFGLLRS NPVCNKRVLT KYARVVKDPD
     PIATTQLLMG QKYPILAKEK YLHHFTPEER AANFAEEEDC RRPGWKRCTR KALNLWLDKE
     IEPNEETKQV IKVLYENSFD LVRDYLSHSW DRAIIRYGIV PKERQVVATR KVLVDVPKEY
     RQKAVVEIIQ PGFNLNQPEV KIYVEKIMEK IGHKMVLGMS IVDQARVLLN SLDPKMRMLP
     VAVTLHDNLA QHSISYYGNN WMVHHLPGRV YDTPGDTNDL REACSLIMRE VLKVKPEKRR
     DHLLHLRKGP QGLLEVLQET KENYPVKVIK SLLGLPCSKS HDYFGTQMII EAAVEESRLV
     TSGGGVSWRE YTGIERIHFR HDDVRGWYTH DGPLIKEIVF SRTERKTFTK LLVNIANYIH
     YDWAARPAKT AKEMRRLTME EVMLSPWSFL GATRGIWQEF WARLDSSYFP TSEEIRNYLQ
     MGDGEIKIRC VLETEVPGDT SVYYRVTDEG ALVEVGGSSR IPKIESLPKR SSKTLDGTWN
     SNDMTFIPIL HPTVCLPRTI EFHYSRPDLL SDIQCFNFSN YNQHCPYLLP ANERASFCNT
     ARMMLYGAST QVRRWPKVYL AYLYCFAGFH ETPTVSKLHR TRFLTYDEID LYAQRGVFKH
     NPENDTWLIF NKEWVGLNDP FQFPGLLVSS ALLGYRLENV THAGKRKRED DLMDLKDGMK
     ELESKMDGSS VYV