PBAN_APIME
ID PBAN_APIME Reviewed; 195 AA.
AC A8CL69;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 25-MAY-2022, entry version 44.
DE RecName: Full=PBAN-type neuropeptides;
DE AltName: Full=Pheromone/pyrokinin biosynthesis-activating neuropeptide;
DE Contains:
DE RecName: Full=TSQDITSGMWFGPRL-amide;
DE AltName: Full=Pyrokinin-1;
DE Contains:
DE RecName: Full=QITQFTPRL-amide;
DE AltName: Full=Pyrokinin-2;
DE Contains:
DE RecName: Full=IYLPLFASRL-amide;
DE Contains:
DE RecName: Full=VPWTPSPRL-amide;
DE Flags: Precursor;
GN Name=PBAN {ECO:0000303|PubMed:17068263};
OS Apis mellifera (Honeybee).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=7460;
RN [1] {ECO:0000312|EMBL:ABB05501.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Sonne A.T., Grimmelikhuijzen C.J.P.;
RT "Molecular cloning of the pyrokinin preprohormone from Apis mellifera.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 66-80; 120-128; 154-163 AND 167-175, SUBCELLULAR
RP LOCATION, AMIDATION AT LEU-80; LEU-128; LEU-163 AND LEU-175, PYROGLUTAMATE
RP FORMATION AT GLN-120, AND MASS SPECTROMETRY.
RC TISSUE=Brain {ECO:0000269|PubMed:17068263};
RX PubMed=17068263; DOI=10.1126/science.1124128;
RA Hummon A.B., Richmond T.A., Verleyen P., Baggerman G., Huybrechts J.,
RA Ewing M.A., Vierstraete E., Rodriguez-Zas S.L., Schoofs L., Robinson G.E.,
RA Sweedler J.V.;
RT "From the genome to the proteome: uncovering peptides in the Apis brain.";
RL Science 314:647-649(2006).
CC -!- FUNCTION: A hormone that controls sex pheromone production in females
CC and pheromone responsiveness in male. Also mediates visceral muscle
CC contractile activity (myotropic activity) (By similarity).
CC {ECO:0000250|UniProtKB:P09971, ECO:0000269|PubMed:17068263}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17068263}.
CC -!- MASS SPECTROMETRY: [TSQDITSGMWFGPRL-amide]: Mass=1693.82; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [QITQFTPRL-amide]: Mass=1101.63; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [QITQFTPRL-amide]: Mass=1084.60; Method=MALDI;
CC Note=Lacking the pyrrolidone carboxylic acid modification.;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [IYLPLFASRL-amide]: Mass=1190.72; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [VPWTPSPRL-amide]: Mass=1050.60; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- SIMILARITY: Belongs to the pyrokinin family. {ECO:0000255}.
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DR EMBL; DQ199622; ABB05501.1; -; mRNA.
DR RefSeq; NP_001104182.1; NM_001110712.2.
DR AlphaFoldDB; A8CL69; -.
DR STRING; 7460.GB46057-PA; -.
DR PaxDb; A8CL69; -.
DR EnsemblMetazoa; NM_001110712; NP_001104182; GeneID_100126690.
DR GeneID; 100126690; -.
DR KEGG; ame:100126690; -.
DR CTD; 692749; -.
DR eggNOG; ENOG502SEUG; Eukaryota.
DR HOGENOM; CLU_120628_0_0_1; -.
DR InParanoid; A8CL69; -.
DR OMA; ITSGMWF; -.
DR OrthoDB; 1434163at2759; -.
DR PhylomeDB; A8CL69; -.
DR Proteomes; UP000005203; Unplaced.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0016084; F:myostimulatory hormone activity; ISS:UniProtKB.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISS:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; ISS:UniProtKB.
DR InterPro; IPR001484; Pyrokinin_CS.
DR PROSITE; PS00539; PYROKININ; 2.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Hormone; Neuropeptide; Pyrrolidone carboxylic acid; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000269|PubMed:17068263"
FT PROPEP 34..63
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339265"
FT PEPTIDE 66..80
FT /note="TSQDITSGMWFGPRL-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339266"
FT PROPEP 86..117
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339267"
FT PEPTIDE 120..128
FT /note="QITQFTPRL-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339268"
FT PROPEP 131..153
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339269"
FT PEPTIDE 154..163
FT /note="IYLPLFASRL-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339270"
FT PEPTIDE 167..175
FT /note="VPWTPSPRL-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339271"
FT PROPEP 178..195
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000339272"
FT MOD_RES 80
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 120
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 128
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 163
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 175
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
SQ SEQUENCE 195 AA; 22455 MW; 0FF4096BF7431BE2 CRC64;
MIGFAVFSSF NRFTTIFVCV LLCVVYLLSY ASGEYDGRDS SSGSNNDRAP SNEFGSCTDG
KCIKRTSQDI TSGMWFGPRL GRRRRADRKP EINSDIEAFA NAFEEPHWAI VTIPETEKRQ
ITQFTPRLGR ESGEDYFSYG FPKDQEELYT EEQIYLPLFA SRLGRRVPWT PSPRLGRQLH
NIVDKPRQNF NDPRF
