PBCE_EMENI
ID PBCE_EMENI Reviewed; 333 AA.
AC A0A1U8QP15; C8VN89; Q5BCY4;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=Oxidoreductase AN1596 {ECO:0000303|PubMed:22506079};
DE EC=1.-.-.- {ECO:0000305|PubMed:22506079};
DE AltName: Full=Pimaradiene biosynthesis cluster protein AN1596 {ECO:0000303|PubMed:22506079};
GN ORFNames=AN1596, ANIA_01596;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=22506079; DOI=10.1371/journal.pone.0035450;
RA Bromann K., Toivari M., Viljanen K., Vuoristo A., Ruohonen L.,
RA Nakari-Setaelae T.;
RT "Identification and characterization of a novel diterpene gene cluster in
RT Aspergillus nidulans.";
RL PLoS ONE 7:e35450-e35450(2012).
RN [4]
RP FUNCTION.
RX PubMed=27098256; DOI=10.1007/s00253-016-7517-5;
RA Bromann K., Toivari M., Viljanen K., Ruohonen L., Nakari-Setaelae T.;
RT "Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-
RT terpinene production.";
RL Appl. Microbiol. Biotechnol. 100:6345-6359(2016).
CC -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC biosynthesis of the diterpene ent-pimara-8(14),15-diene (PD)
CC (PubMed:22506079, PubMed:27098256). Within the cluster, the HMG-CoA
CC reductase AN1593 functions in the mevalonate pathway, which produces
CC isoprenoid precursors (PubMed:22506079, PubMed:27098256). The
CC geranylgeranyl pyrophosphate (GGPP) synthase AN1592 is needed in the
CC formation of GGPP, the precursor for diterpenes (PubMed:22506079,
CC PubMed:27098256). Lastly, the pimaradiene synthase pbcA performs the 2
CC cyclization steps that convert GGPP to ent-pimara-8(14),15-diene
CC (PubMed:22506079, PubMed:27098256). The putative roles of the remaining
CC cluster enzymes in ent-pimara-8(14),15-diene biosynthesis is unclear
CC (Probable). The cytochrome P450 monooxygenase AN1598, the glutathione
CC S-transferase AN1595, the oxidoreductases AN1596 and AN1597 probably
CC function as decorative enzymes (Probable). It is possible that in
CC biological conditions the compound is oxidized to ent-pimara-8(14),15-
CC dien-19-oic acid, which is a bioactive diterpene compound predominant
CC in many plant extracts (Probable). {ECO:0000269|PubMed:22506079,
CC ECO:0000269|PubMed:27098256, ECO:0000305|PubMed:22506079}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305|PubMed:22506079}.
CC -!- INDUCTION: Expression is positively regulated by the cluster-specific
CC transcription factor pbcR. {ECO:0000269|PubMed:22506079}.
CC -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; AACD01000025; EAA64303.1; -; Genomic_DNA.
DR EMBL; BN001307; CBF85184.1; -; Genomic_DNA.
DR RefSeq; XP_659200.1; XM_654108.1.
DR AlphaFoldDB; A0A1U8QP15; -.
DR SMR; A0A1U8QP15; -.
DR EnsemblFungi; CBF85184; CBF85184; ANIA_01596.
DR EnsemblFungi; EAA64303; EAA64303; AN1596.2.
DR GeneID; 2874630; -.
DR KEGG; ani:AN1596.2; -.
DR VEuPathDB; FungiDB:AN1596; -.
DR eggNOG; KOG1208; Eukaryota.
DR HOGENOM; CLU_044999_0_0_1; -.
DR OMA; YAELPFY; -.
DR OrthoDB; 1164708at2759; -.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000000560; Chromosome VII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1..333
FT /note="Oxidoreductase AN1596"
FT /id="PRO_0000450843"
SQ SEQUENCE 333 AA; 36962 MW; 67C7CD0521CB68BC CRC64;
MVSLKTVQAS NAGLRALPNI TALFVGGTSG IGQSTLRQLA RYADSPTAYI IGRNEARTRP
FLSELQQLNP KGRFHFIEAD VSLVRNVDAA CQQILQQQKK LNFLFMTPGG ISLGGRNETV
EGIDYLFALR YYSRMRFIQN LLPLLEASSP SRVISVYGGG FEYSINTADL DLKHNFSLLN
AYKHSITMTS LSMEHLARTH PAVSFIHVYP GLVGTNIYTN SFPAPVSTFY NYLVWPFMKP
FSVDLGESGE RHLFHLSSAH YPAKQGIVPQ GVPLEAGEVA KGITGEPGSG AYLLNWKGDV
RPSTKILAQY REQKIPQLVW DHTESLMDQA VHR
