PBCF_EMENI
ID PBCF_EMENI Reviewed; 295 AA.
AC A0A1U8QJR1; C8VN90; Q5BCY3;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=Oxidoreductase AN1597 {ECO:0000303|PubMed:22506079};
DE EC=1.-.-.- {ECO:0000305|PubMed:22506079};
DE AltName: Full=Pimaradiene biosynthesis cluster protein AN1597 {ECO:0000303|PubMed:22506079};
GN ORFNames=AN1597, ANIA_01597;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=22506079; DOI=10.1371/journal.pone.0035450;
RA Bromann K., Toivari M., Viljanen K., Vuoristo A., Ruohonen L.,
RA Nakari-Setaelae T.;
RT "Identification and characterization of a novel diterpene gene cluster in
RT Aspergillus nidulans.";
RL PLoS ONE 7:e35450-e35450(2012).
RN [4]
RP FUNCTION.
RX PubMed=27098256; DOI=10.1007/s00253-016-7517-5;
RA Bromann K., Toivari M., Viljanen K., Ruohonen L., Nakari-Setaelae T.;
RT "Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-
RT terpinene production.";
RL Appl. Microbiol. Biotechnol. 100:6345-6359(2016).
CC -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC biosynthesis of the diterpene ent-pimara-8(14),15-diene (PD)
CC (PubMed:22506079, PubMed:27098256). Within the cluster, the HMG-CoA
CC reductase AN1593 functions in the mevalonate pathway, which produces
CC isoprenoid precursors (PubMed:22506079, PubMed:27098256). The
CC geranylgeranyl pyrophosphate (GGPP) synthase AN1592 is needed in the
CC formation of GGPP, the precursor for diterpenes (PubMed:22506079,
CC PubMed:27098256). Lastly, the pimaradiene synthase pbcA performs the 2
CC cyclization steps that convert GGPP to ent-pimara-8(14),15-diene
CC (PubMed:22506079, PubMed:27098256). The putative roles of the remaining
CC cluster enzymes in ent-pimara-8(14),15-diene biosynthesis is unclear
CC (Probable). The cytochrome P450 monooxygenase AN1598, the glutathione
CC S-transferase AN1595, the oxidoreductases AN1596 and AN1597 probably
CC function as decorative enzymes (Probable). It is possible that in
CC biological conditions the compound is oxidized to ent-pimara-8(14),15-
CC dien-19-oic acid, which is a bioactive diterpene compound predominant
CC in many plant extracts (Probable). {ECO:0000269|PubMed:22506079,
CC ECO:0000269|PubMed:27098256, ECO:0000305|PubMed:22506079}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305|PubMed:22506079}.
CC -!- INDUCTION: Expression is positively regulated by the cluster-specific
CC transcription factor pbcR. {ECO:0000269|PubMed:22506079}.
CC -!- SIMILARITY: Belongs to the asaB hydroxylase/desaturase family.
CC {ECO:0000305}.
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DR EMBL; AACD01000025; EAA64304.1; -; Genomic_DNA.
DR EMBL; BN001307; CBF85186.1; -; Genomic_DNA.
DR RefSeq; XP_659201.1; XM_654109.1.
DR AlphaFoldDB; A0A1U8QJR1; -.
DR SMR; A0A1U8QJR1; -.
DR EnsemblFungi; CBF85186; CBF85186; ANIA_01597.
DR EnsemblFungi; EAA64304; EAA64304; AN1597.2.
DR GeneID; 2875703; -.
DR KEGG; ani:AN1597.2; -.
DR eggNOG; ENOG502RYCS; Eukaryota.
DR HOGENOM; CLU_042688_2_0_1; -.
DR OMA; NYVMSHG; -.
DR OrthoDB; 867824at2759; -.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000000560; Chromosome VII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR044053; AsaB-like.
DR PANTHER; PTHR34598; PTHR34598; 1.
PE 2: Evidence at transcript level;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1..295
FT /note="Oxidoreductase AN1597"
FT /id="PRO_0000450844"
SQ SEQUENCE 295 AA; 33101 MW; 7A37117C014B87A8 CRC64;
MVDATSPPGV NAVVNYYVPN SDGSPPATND MAVMLGQKDM ISHKMRIRDL RPYKEEYSLD
RNGFQYATIH STLTDATDET QIKEVYYREI EKLVQDITGA KRVLAFHHAV RTRTGNEFGE
QIKDRYQGVE GPAYRVHIDQ TPQGALSIVQ FMFPDLADDV RNGSFQVINV WRPLTRVQRD
PLMVADAAEM PPEDLLLISR KYYNGLHSSN FVIKYDGRMA AGEGPTDGLS GDGKHSWWYI
GDQEPTEALV FSSSGFRNGK AIIGTAHDLY SAEPMINAYE RKNASSLYGH DESQI
