ID   PBCR_EMENI              Reviewed;         746 AA.
AC   A0A1U8QL22; C8VN92; Q5BCY1;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Transcription factor pbcR {ECO:0000303|PubMed:22506079};
DE   AltName: Full=Pimaradiene biosynthesis cluster protein R {ECO:0000303|PubMed:22506079};
GN   Name=pbcR {ECO:0000303|PubMed:22506079}; ORFNames=AN1599, ANIA_01599;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [3]
RP   FUNCTION.
RX   PubMed=22506079; DOI=10.1371/journal.pone.0035450;
RA   Bromann K., Toivari M., Viljanen K., Vuoristo A., Ruohonen L.,
RA   Nakari-Setaelae T.;
RT   "Identification and characterization of a novel diterpene gene cluster in
RT   Aspergillus nidulans.";
RL   PLoS ONE 7:e35450-e35450(2012).
RN   [4]
RP   FUNCTION.
RX   PubMed=27098256; DOI=10.1007/s00253-016-7517-5;
RA   Bromann K., Toivari M., Viljanen K., Ruohonen L., Nakari-Setaelae T.;
RT   "Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-
RT   terpinene production.";
RL   Appl. Microbiol. Biotechnol. 100:6345-6359(2016).
CC   -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC       the biosynthesis of the diterpene ent-pimara-8(14),15-diene (PD)
CC       (PubMed:22506079, PubMed:27098256). Acts as a positive regulator for
CC       the cluster gene (PubMed:22506079). Down-regulates the expression of
CC       the penicillin gene cluster, two putative polyketide clusters, and one
CC       putative nonribosomal peptide cluster (PubMed:22506079).
CC       {ECO:0000269|PubMed:22506079, ECO:0000269|PubMed:27098256}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
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DR   EMBL; AACD01000025; EAA64306.1; -; Genomic_DNA.
DR   EMBL; BN001307; CBF85190.1; -; Genomic_DNA.
DR   RefSeq; XP_659203.1; XM_654111.1.
DR   AlphaFoldDB; A0A1U8QL22; -.
DR   EnsemblFungi; CBF85190; CBF85190; ANIA_01599.
DR   EnsemblFungi; EAA64306; EAA64306; AN1599.2.
DR   GeneID; 2875702; -.
DR   KEGG; ani:AN1599.2; -.
DR   eggNOG; ENOG502QRYY; Eukaryota.
DR   HOGENOM; CLU_006524_0_2_1; -.
DR   OMA; CHYICSN; -.
DR   OrthoDB; 644551at2759; -.
DR   Proteomes; UP000000560; Chromosome VII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..746
FT                   /note="Transcription factor pbcR"
FT                   /id="PRO_0000450846"
FT   DNA_BIND        47..76
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          109..131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   746 AA;  82833 MW;  5632D1EB878C61F5 CRC64;
     MYPWSSTGTS PFSHPDNEGA ESGDMSMGEE QQQPHQRRQK FNNLRACQSC RASKVRCDQP
     NPGMPCLRCQ KSGKPCVDAA SQPGKRQRQP INSILEMESR IETILSSAEL QDSAGDGETA
     HSTALRSPSQ LSHHIQPFQH LPMGFAIPFN GGNSGTEDLN SSIRSWLNDN ITDLDARTTE
     TIFSHYLTNM VPTFPVVVFA TGTTAADVRR NNPILFLAIL DVASSGFCAL ETQRKLRKLI
     VQAYVHCMLR TEQYTLGLLQ ALIVSATWYR TIEPVEPGEQ MDIYQISHTA ANMALIMRLG
     ESLNAKSWGG PMFPRREMKK GPGSAFQADS LEARRVWLGC HYICSNTSMS LRAPNIMRWT
     RLMDECLEVL ENSPAALLSD RLLCQHIRLQ HITEEFAMHL SAEEASAPAK SRAIQIQVTH
     RAFKRQLSEW RRTVGDGWDA HCTATSDDVP EDNAQRLTPP PPIVAIEPHA ITEFMDTIDN
     IFRVFTSLDM STIRALPAMY LIRIIYTFII LVKLYFAAAK LPAQDAVLQV DGLQVSRRFN
     RVIQMTAGWG PLWPATKLTT VFTKMRSWFE SGGDNNCQRL QQAAAWLTGW ELKPPSQGRD
     AHAMNMAEVV SDDGSIVASS SRGPASWVPS LASTDVDTLA FSHEPPLGTE FSIAPPPFRS
     MSCATKSCSP QAGAAEFMHD EEVPLEGQRL GDLPNIDQMD DVGMDWSQYT NMGFDLYNLD
     APFLPNPPSG FDPDAAMKDN CADRNT