ID   PBL1F_EUGGR             Reviewed;         859 AA.
AC   P84742; Q2P9M5;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Photoactivated adenylate cyclase subunit beta-like protein 1224-5/1F;
GN   Name=pacB {ECO:0000312|EMBL:CAJ57399.1};
OS   Euglena gracilis.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAJ57399.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=SAG 1224-5/1F;
RX   PubMed=16121285; DOI=10.1039/b502002f;
RA   Ntefidou M., Haeder D.-P.;
RT   "Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena
RT   gracilis mutant strains.";
RL   Photochem. Photobiol. Sci. 4:732-739(2005).
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC       {ECO:0000250|UniProtKB:Q8S9F1}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC       {ECO:0000269|PubMed:16121285}. Note=Paraxonemal body. And paraxonemal
CC       bodies (PABs).
CC   -!- MISCELLANEOUS: The 1224-5/1F strain is deficient in phototaxis. It is
CC       not known if this is due to defective adenylate cyclase activity or
CC       defective BLUF domains in this protein. {ECO:0000269|PubMed:16121285}.
CC   -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC       family. {ECO:0000255|PROSITE-ProRule:PRU00099}.
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DR   EMBL; AM181340; CAJ57399.1; -; mRNA.
DR   AlphaFoldDB; P84742; -.
DR   SMR; P84742; -.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0009882; F:blue light photoreceptor activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd07302; CHD; 1.
DR   Gene3D; 3.30.70.1230; -; 2.
DR   InterPro; IPR001054; A/G_cyclase.
DR   InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR   InterPro; IPR007024; BLUF_domain.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   Pfam; PF04940; BLUF; 2.
DR   Pfam; PF00211; Guanylate_cyc; 1.
DR   SMART; SM01034; BLUF; 2.
DR   SUPFAM; SSF54975; SSF54975; 2.
DR   SUPFAM; SSF55073; SSF55073; 2.
DR   PROSITE; PS50925; BLUF; 2.
DR   PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Flagellum; Repeat.
FT   CHAIN           1..859
FT                   /note="Photoactivated adenylate cyclase subunit beta-like
FT                   protein 1224-5/1F"
FT                   /id="PRO_0000195722"
FT   DOMAIN          56..149
FT                   /note="BLUF 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          473..565
FT                   /note="BLUF 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          620..748
FT                   /note="Guanylate cyclase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   REGION          426..445
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          835..859
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   859 AA;  94650 MW;  0B29D9D628A3094E CRC64;
     MYILVWKKGQ QIKTFHTLDE AAQFKAASNI DEAQVFSVTD APDISASGGS NEARNRRRLM
     YLTNSTNPEE CNPQFVGEMA RVATIQNREI GARGFLMYSC RFLLQVIEET VEDPDSLFAK
     FSPEPRHERW IELDNGPCTG RMYGDWNMKD SHLDSITTHP AMLTILFQMA RSISCLWSYL
     QKSERIMLLF GKDPGAQRPE PMRVVVTFTY LVDFGSMLSI PNLTEQAAEV LTTFVDGCVK
     NVEGSGGSIA KFTTGICMAQ WPINRTVEAL TAIQQISEDL AQLRSQQAPG SAVSLMYSQA
     GLHYGRGMLC NAGSRKSDFT LLDFINTTSR IATLANKLKT PLLFHSFDVR CLLGDEMREE
     IEGAGMHQVK GRDKPVGVYQ FPGPELDVEM VLRQKVEQFT PGRFRCQMPV VEYEALPIWQ
     RPPIVDDTPK GYPRPRTMPG YGGRQRSDSL VDRLIMIAKL AGPSVSATGD TTLTTLTYIS
     QATRKPMSRL HLSALMGTAT RRNAQQSITG TLLDVNGLFG QTLEGPKVAV VMYLSIRQDA
     RQTDVTTVHM APLQERVCPS EWTLTSATEE MLATFPPLQD VLSQLAKSCT SLETYVRSTV
     GYLTAGNNRR NLMPVSCGVV MLATDIGSFT SLTEESSLTE VWMICNTFID ACTSAVCQEG
     GEVIKRIGDC VTAYFPGNNA ESAVAAAQEL FTFCRQLREA FVDVVDVRGC VSCGVGLDYS
     QVVMAQCGSL GLTEYVVAGV SARVMEVDAM TREVGYAIGV TEPYVTDRLS PQSRDHGIVP
     TPQAIELPCY GIAGEQLELD VDSIKRGIKA LHAARSGEMP VTEPEEALRL FRVSPGRVRH
     GDTGRRSNSA QGKRSIQVR