ID   PBLST_EUGGR             Reviewed;         859 AA.
AC   P84743; Q2P9M4;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Photoactivated adenylate cyclase subunit beta-like protein ST-;
GN   Name=pacB {ECO:0000312|EMBL:CAJ57400.1};
OS   Euglena gracilis.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAJ57400.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=ST- {ECO:0000269|PubMed:16121285};
RX   PubMed=16121285; DOI=10.1039/b502002f;
RA   Ntefidou M., Haeder D.-P.;
RT   "Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena
RT   gracilis mutant strains.";
RL   Photochem. Photobiol. Sci. 4:732-739(2005).
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC       {ECO:0000250|UniProtKB:Q8S9F1}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC       {ECO:0000269|PubMed:16121285}.
CC   -!- MISCELLANEOUS: The ST- strain is deficient in phototaxis. It is not
CC       known if this is due to defective adenylate cyclase activity or
CC       defective BLUF domains in this protein. In wild-type E.gracilis,
CC       photoactivated adenylate cyclase is found in the paraxonemal bodies
CC       (PABs). PABs are absent from this strain.
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DR   EMBL; AM181341; CAJ57400.1; -; mRNA.
DR   AlphaFoldDB; P84743; -.
DR   SMR; P84743; -.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0009882; F:blue light photoreceptor activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd07302; CHD; 2.
DR   Gene3D; 3.30.70.1230; -; 2.
DR   InterPro; IPR001054; A/G_cyclase.
DR   InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR   InterPro; IPR007024; BLUF_domain.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   Pfam; PF04940; BLUF; 2.
DR   Pfam; PF00211; Guanylate_cyc; 1.
DR   SMART; SM01034; BLUF; 2.
DR   SUPFAM; SSF54975; SSF54975; 2.
DR   SUPFAM; SSF55073; SSF55073; 2.
DR   PROSITE; PS50925; BLUF; 2.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Flagellum; Repeat.
FT   CHAIN           1..859
FT                   /note="Photoactivated adenylate cyclase subunit beta-like
FT                   protein ST-"
FT                   /id="PRO_0000195725"
FT   DOMAIN          56..149
FT                   /note="BLUF 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          471..563
FT                   /note="BLUF 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   REGION          420..444
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          814..859
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        830..844
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        845..859
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   859 AA;  94790 MW;  BA0B9E98BA3C62A2 CRC64;
     MYILVWKKGQ QIKTFHTLDE AAQFKAASNI DEAQMFSVTV ASAISASGGS TEATNLRRLM
     YLSKSTNPEE CNPQFLAEMA RVAFIRNREI RVSGFLMYSS PFFIQVIVET DEDLDFLFAK
     ISADPRHEMC IVLANGPGTG RMYGDWHMKD SHLDSITTHT AMKTILYQIA RSFSSMWIYL
     QKSAANMLML GKDPAAQPPE RRSVVVTFIY LVEFCSIFSH PNLTEKTADV LSTFVYVCVK
     NVEGSGGNIA KFIPAICMTY WPIIRTKEAL TAIQQISEDL AQLRSQQAQG SAVSLRYSQA
     GVHYGRGMQC NAGSRKSDFT LLGDCINTTS RIATLAKKLK TPLLFGFEVR CLLGDEKREE
     IEGAGIHQVK GRDKPVVVYQ FPGPDLDVAM VRQKIEQFTP GRFRCQMPVV EYEALPISQR
     PPIFDDTPKS NPRPRTPGYG GRQRSDSLVD RLIRIANLSG FSESATGDTT LTTLTYISQA
     TRPMSRLDLS AIMPTATRRN AQQSNIGTVL HENGLFVQTL EGPKDAVVNL YLRIRQDPRH
     TDVTTVHMAP LQERVYTSEW TLTLATEEML ATFPPVQDVL SQLAKSYTSL ETYVPSTVVR
     YLTAGNNPRN LMPVSCGVVM LATDISSFTS LTEISSFTEV WMICNTFIDA CTSAICQEGG
     EVIKLIGDCV TPYFPGNNAD LAVAAAQELF IFCRQLREAF VDVLDVRGCV SCAVGMDYGQ
     VVMAQCGLLR LSDYVAAGAV SAGVVEVEAI TREVGYQFVV TVPDADRVQP QFRDYGIEPT
     PQAIGGLPCY GIVREVFDLP VESIKRGIKA MHAARSGEQP LTEPEQAKPD FRVSPGRDRH
     GVSGRRSNSS QGKGSIQVG