ID   ASPA_PSEAE              Reviewed;         474 AA.
AC   Q9HTD7;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Aspartate ammonia-lyase;
DE            Short=Aspartase;
DE            EC=4.3.1.1;
GN   Name=aspA; OrderedLocusNames=PA5429;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-aspartate = fumarate + NH4(+); Xref=Rhea:RHEA:16601,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29806, ChEBI:CHEBI:29991; EC=4.3.1.1;
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family.
CC       Aspartase subfamily. {ECO:0000305}.
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DR   EMBL; AE004091; AAG08814.1; -; Genomic_DNA.
DR   PIR; C82968; C82968.
DR   RefSeq; NP_254116.1; NC_002516.2.
DR   RefSeq; WP_003096822.1; NZ_QZGE01000012.1.
DR   AlphaFoldDB; Q9HTD7; -.
DR   SMR; Q9HTD7; -.
DR   STRING; 287.DR97_2806; -.
DR   PaxDb; Q9HTD7; -.
DR   PRIDE; Q9HTD7; -.
DR   EnsemblBacteria; AAG08814; AAG08814; PA5429.
DR   GeneID; 878603; -.
DR   KEGG; pae:PA5429; -.
DR   PATRIC; fig|208964.12.peg.5690; -.
DR   PseudoCAP; PA5429; -.
DR   HOGENOM; CLU_021594_4_1_6; -.
DR   InParanoid; Q9HTD7; -.
DR   OMA; EICENYV; -.
DR   PhylomeDB; Q9HTD7; -.
DR   BioCyc; PAER208964:G1FZ6-5556-MON; -.
DR   BRENDA; 4.3.1.1; 5087.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008797; F:aspartate ammonia-lyase activity; IBA:GO_Central.
DR   GO; GO:0006531; P:aspartate metabolic process; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.10.275.10; -; 1.
DR   InterPro; IPR004708; ApsA.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR018951; Fumarase_C_C.
DR   InterPro; IPR020557; Fumarate_lyase_CS.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   Pfam; PF10415; FumaraseC_C; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR00839; aspA; 1.
DR   PROSITE; PS00163; FUMARATE_LYASES; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome.
FT   CHAIN           1..474
FT                   /note="Aspartate ammonia-lyase"
FT                   /id="PRO_0000287771"
FT   BINDING         105
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         144..146
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   474 AA;  51070 MW;  DE868A8CA8478429 CRC64;
     MSPVASSRIE KDLLGTLEVP ADAYYGIQTL RAVNNFRLSG VPLSHYPKLV VALAMVKQAA
     ADANRQLGHL PEDKHAAISE ACARLIRGDF HEQFVVDMIQ GGAGTSTNMN ANEVIANIAL
     EAMGHTKGEY KYLHPNNDVN MAQSTNDAYP TAIRLGLLLG HDTLLASLDS LIQAFAAKGV
     EFAGVLKMGR TQLQDAVPMT LGQEFHAFAT TLGEDLDRLR RLAPELLTEV NLGGTAIGTG
     INADPGYQKL AVERLAAISG QPLKPAADLI EATSDMGAFV LFSGMLKRTA VKLSKICNDL
     RLLSSGPRTG INEINLPPRQ PGSSIMPGKV NPVIPEAVNQ VAFEVIGNDL ALTLAAEGGQ
     LQLNVMEPLI AYKIFDSIRL LQRAMDMLRE HCITGITANV ERCHELVEHS IGLVTALNPY
     IGYENSTRIA KTALESGRGV LELVREEKLL DEATLADILL PENMIAPRLI PLRA