PBP1_ARATH
ID PBP1_ARATH Reviewed; 127 AA.
AC Q9LSQ6; Q8LG35;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Calcium-binding protein PBP1;
DE AltName: Full=KIC-related protein 2;
DE AltName: Full=PINOID-binding protein 1;
GN Name=PBP1; Synonyms=KRP2; OrderedLocusNames=At5g54490; ORFNames=F24B18.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=14688294; DOI=10.1105/tpc.016600;
RA Reddy V.S., Day I.S., Thomas T., Reddy A.S.N.;
RT "KIC, a novel Ca2+ binding protein with one EF-hand motif, interacts with a
RT microtubule motor protein and regulates trichome morphogenesis.";
RL Plant Cell 16:185-200(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH PID, AND INDUCTION BY AUXIN.
RX PubMed=12857841; DOI=10.1104/pp.103.019943;
RA Benjamins R., Ampudia C.S., Hooykaas P.J., Offringa R.;
RT "PINOID-mediated signaling involves calcium-binding proteins.";
RL Plant Physiol. 132:1623-1630(2003).
CC -!- FUNCTION: Potential calcium sensor that binds calcium in vitro.
CC {ECO:0000269|PubMed:14688294}.
CC -!- SUBUNIT: Interacts with PID. {ECO:0000269|PubMed:12857841}.
CC -!- INTERACTION:
CC Q9LSQ6; O64682: PID; NbExp=4; IntAct=EBI-1393367, EBI-1393382;
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:12857841}.
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DR EMBL; AY363868; AAR16086.1; -; mRNA.
DR EMBL; AB026634; BAA97522.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96501.1; -; Genomic_DNA.
DR EMBL; BT024572; ABD38911.1; -; mRNA.
DR EMBL; AY084485; AAM61056.1; -; mRNA.
DR RefSeq; NP_200260.1; NM_124829.2.
DR AlphaFoldDB; Q9LSQ6; -.
DR SMR; Q9LSQ6; -.
DR BioGRID; 20781; 2.
DR IntAct; Q9LSQ6; 2.
DR STRING; 3702.AT5G54490.1; -.
DR PaxDb; Q9LSQ6; -.
DR PRIDE; Q9LSQ6; -.
DR EnsemblPlants; AT5G54490.1; AT5G54490.1; AT5G54490.
DR GeneID; 835537; -.
DR Gramene; AT5G54490.1; AT5G54490.1; AT5G54490.
DR KEGG; ath:AT5G54490; -.
DR Araport; AT5G54490; -.
DR TAIR; locus:2147294; AT5G54490.
DR eggNOG; KOG0028; Eukaryota.
DR HOGENOM; CLU_137017_1_0_1; -.
DR OMA; ELLMDKD; -.
DR OrthoDB; 1495109at2759; -.
DR PhylomeDB; Q9LSQ6; -.
DR PRO; PR:Q9LSQ6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LSQ6; baseline and differential.
DR Genevisible; Q9LSQ6; AT.
DR GO; GO:0005509; F:calcium ion binding; ISS:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR044205; KIC/PBP1/KRP1.
DR PANTHER; PTHR47319; PTHR47319; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 1: Evidence at protein level;
KW Calcium; Metal-binding; Reference proteome.
FT CHAIN 1..127
FT /note="Calcium-binding protein PBP1"
FT /id="PRO_0000411974"
FT DOMAIN 72..107
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 85
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 87
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 89
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 96
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 5
FT /note="K -> T (in Ref. 5; AAM61056)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 127 AA; 14468 MW; 21ABDD4955EB21A5 CRC64;
MASPKSSTRP NQENQEPQFQ DFFPTMAGKL GGEGLIEEIC KGFELLMDKD KGVITFESLR
RNASTVLGLG DLTDDDVRYM INEGDFDRDG ALNQMEFCVL MFRLSPELME ASRCVVTEVI
EEEFYRH
