PBP5_PSEAE
ID PBP5_PSEAE Reviewed; 310 AA.
AC P72161;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=D-alanyl-D-alanine endopeptidase;
DE Short=DD-endopeptidase;
DE EC=3.4.21.-;
DE AltName: Full=Penicillin-binding protein 5;
DE Short=PBP-5;
DE Flags: Precursor;
GN Name=pbpG; OrderedLocusNames=PA0869;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RA Song J., Jensen R.A.;
RL Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Cell wall formation.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase S11 family. {ECO:0000305}.
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DR EMBL; U62582; AAC46341.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04258.1; -; Genomic_DNA.
DR PIR; B83538; B83538.
DR RefSeq; NP_249560.1; NC_002516.2.
DR RefSeq; WP_003114239.1; NZ_QZGE01000007.1.
DR AlphaFoldDB; P72161; -.
DR SMR; P72161; -.
DR STRING; 287.DR97_1074; -.
DR MEROPS; S11.002; -.
DR PaxDb; P72161; -.
DR PRIDE; P72161; -.
DR DNASU; 877694; -.
DR EnsemblBacteria; AAG04258; AAG04258; PA0869.
DR GeneID; 877694; -.
DR KEGG; pae:PA0869; -.
DR PATRIC; fig|208964.12.peg.903; -.
DR PseudoCAP; PA0869; -.
DR HOGENOM; CLU_027070_0_3_6; -.
DR InParanoid; P72161; -.
DR OMA; WDIKLTK; -.
DR PhylomeDB; P72161; -.
DR BioCyc; PAER208964:G1FZ6-884-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IDA:CACAO.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 3.40.710.10; -; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR018044; Peptidase_S11.
DR InterPro; IPR001967; Peptidase_S11_N.
DR Pfam; PF00768; Peptidase_S11; 1.
DR PRINTS; PR00725; DADACBPTASE1.
DR SUPFAM; SSF56601; SSF56601; 1.
PE 3: Inferred from homology;
KW Cell shape; Cell wall biogenesis/degradation; Hydrolase;
KW Peptidoglycan synthesis; Periplasm; Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..310
FT /note="D-alanyl-D-alanine endopeptidase"
FT /id="PRO_0000027239"
FT ACT_SITE 66
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 69
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 123
FT /evidence="ECO:0000250"
FT BINDING 230
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 296
FT /note="D -> DD (in Ref. 1; AAC46341)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 34046 MW; B1F62A3A2F9EF641 CRC64;
MRNRLLSLVT LFLSLSVATA VSASPPPKAG LPLQQELASG SALVTDQNTG KVLYSRNPDL
VVPIASITKL MTAMVVLDSK LPLDEVLPIA ISETSEMRGV FSRVRVGSQI SRRDMLLLAL
MSSENRAAAS LAQNYPGGKN AFVKAMNAKA HALGMKNTRY VEPTGLSLHN VSTARDLTRL
LVASRQYPLL SQWSTTPEKT VAFRHPNYTL GFRNTNHLIN NKTWNIQLTK TGFTNEAGHC
LVMRTTINRS PVNLVVLDAF GKYTHFADAT RLRRWLETGQ VTAIPAAAKA YRLQRDRERG
LGQPVAQAVR
