ID   PBPB_MYCS2              Reviewed;         648 AA.
AC   A0R022;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Penicillin-binding protein PbpB;
DE   AltName: Full=PBP3;
GN   Name=pbpB; Synonyms=ftsI; OrderedLocusNames=MSMEG_4233, MSMEI_4133;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, AND INTERACTION WITH WAG31.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=19496931; DOI=10.1111/j.1365-2958.2009.06750.x;
RA   Mukherjee P., Sureka K., Datta P., Hossain T., Barik S., Das K.P.,
RA   Kundu M., Basu J.;
RT   "Novel role of Wag31 in protection of mycobacteria under oxidative
RT   stress.";
RL   Mol. Microbiol. 73:103-119(2009).
CC   -!- SUBUNIT: Interacts with Wag31. {ECO:0000269|PubMed:19496931}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transpeptidase family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK71440.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP40590.1; -; Genomic_DNA.
DR   RefSeq; WP_011729661.1; NZ_SIJM01000003.1.
DR   RefSeq; YP_888510.1; NC_008596.1.
DR   AlphaFoldDB; A0R022; -.
DR   SMR; A0R022; -.
DR   STRING; 246196.MSMEI_4133; -.
DR   PRIDE; A0R022; -.
DR   EnsemblBacteria; ABK71440; ABK71440; MSMEG_4233.
DR   EnsemblBacteria; AFP40590; AFP40590; MSMEI_4133.
DR   GeneID; 66735578; -.
DR   KEGG; msg:MSMEI_4133; -.
DR   KEGG; msm:MSMEG_4233; -.
DR   PATRIC; fig|246196.19.peg.4153; -.
DR   eggNOG; COG0768; Bacteria.
DR   OMA; HGKEEYY; -.
DR   OrthoDB; 213680at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56519; SSF56519; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..648
FT                   /note="Penicillin-binding protein PbpB"
FT                   /id="PRO_0000422680"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        355
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P0AD65"
SQ   SEQUENCE   648 AA;  69708 MW;  96CCC8D8BCF92BE5 CRC64;
     MSRRGDRPRT PAQPRKKARV DQPRSARTRR TRVSEAEAGL RSSSFVFRHR TGNLAILAVL
     VIAAVQLFML QVPRAAGLRA EAASQLKVTD ITPAIRGSII DRNNDKLAFT IEARALTFQP
     TRVRKQLDEA WRKAQEAGSS TSDDVPNPDE RLNEIAKEIA ARLNNTPDAK TVLKKLKSNE
     TFVYLARAVD PAIANAITDK FPEVGSERQD LRQYPGGSLA ANIVGGIDWD GHGLLGLEDS
     LDAVLAGTDG SVTYDRGSDG VVIPGSYRNR HDAVDGSTVQ LTIDDDIQYH VQQQVQMAKD
     ASGAKNVSAV VLDAKTGEVL AMSNDNTFDP SQDIGRQADR QMGNPSVSSP FEPGSVNKIV
     TAAAAIENGL TNPDEVLQVP GSIHMGGVTV RDAWNHGVMP YTTTGVFGKS SNVGTLMLAQ
     RVGPERFYEM LRKFGLGQRT NVGLPGESSG LLPPIDQWSG SSFSNLPIGQ GLSMTLLQMA
     AMYQTVANDG VRVPPRIIKS TIAPDGTVTE EERPEGIRVI SPETARTLRS MFRSVVQRDP
     MGVQQGTGPQ AAVEGYQIAG KTGTAQQINP ACGCYYDDVY WITFAGIAPA DDPRYVIGIM
     MDAPQRAADG SPGSSAAPLF HEIASWLLQR HNVPLSPDPG PPLTLQAT