ID   PBPX_BACSU              Reviewed;         391 AA.
AC   O31773;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Putative penicillin-binding protein PbpX;
GN   Name=pbpX; OrderedLocusNames=BSU16950;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   INDUCTION BY SIGMA FACTORS.
RC   STRAIN=168 / CU1065;
RX   PubMed=14762009; DOI=10.1128/jb.186.4.1136-1146.2004;
RA   Cao M., Helmann J.D.;
RT   "The Bacillus subtilis extracytoplasmic-function sigmaX factor regulates
RT   modification of the cell envelope and resistance to cationic antimicrobial
RT   peptides.";
RL   J. Bacteriol. 186:1136-1146(2004).
RN   [3]
RP   SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168;
RX   PubMed=15758244; DOI=10.1099/mic.0.27692-0;
RA   Scheffers D.-J.;
RT   "Dynamic localization of penicillin-binding proteins during spore
RT   development in Bacillus subtilis.";
RL   Microbiology 151:999-1012(2005).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}. Note=Localizes to the division septum during
CC       vegetative growth, and accumulates at the prespore during sporulation.
CC       {ECO:0000269|PubMed:15758244}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during vegetative growth and
CC       sporulation. {ECO:0000269|PubMed:15758244}.
CC   -!- INDUCTION: Up-regulated by sigma-X and sigma-W factors.
CC       {ECO:0000269|PubMed:14762009}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene display no visible
CC       phenotype during vegetative growth or sporulation.
CC       {ECO:0000269|PubMed:15758244}.
CC   -!- SIMILARITY: Belongs to the beta-lactamase family. {ECO:0000305}.
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DR   EMBL; AL009126; CAB13568.1; -; Genomic_DNA.
DR   PIR; C69673; C69673.
DR   RefSeq; NP_389577.1; NC_000964.3.
DR   RefSeq; WP_003245877.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O31773; -.
DR   SMR; O31773; -.
DR   STRING; 224308.BSU16950; -.
DR   MEROPS; S12.A21; -.
DR   PaxDb; O31773; -.
DR   PRIDE; O31773; -.
DR   EnsemblBacteria; CAB13568; CAB13568; BSU_16950.
DR   GeneID; 939677; -.
DR   KEGG; bsu:BSU16950; -.
DR   PATRIC; fig|224308.179.peg.1836; -.
DR   eggNOG; COG1680; Bacteria.
DR   InParanoid; O31773; -.
DR   OMA; WWKGYNT; -.
DR   PhylomeDB; O31773; -.
DR   BioCyc; BSUB:BSU16950-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR001466; Beta-lactam-related.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   Pfam; PF00144; Beta-lactamase; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Sporulation; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..391
FT                   /note="Putative penicillin-binding protein PbpX"
FT                   /id="PRO_0000384401"
FT   TRANSMEM        21..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          44..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        44..58
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        59..76
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   391 AA;  43858 MW;  08D3D7AB99826CCE CRC64;
     MTSPTRRRTA KRRRRKLNKR GKLLFGLLAV MVCITIWNAL HRNSEENEPS QETAAVSNTD
     QKKEVKKKTA KKSEEQIKTV DRNQKISNYL KEIGFSGTAM IVRNGEIVTN KGFGYADRKH
     YIQNNPLTSF YVGSSQKALI ATAILQLEEK GKLQTSDPVS TYLPHFPNGQ TITLKNLLTH
     TSGINGHIEG NGAITPDDLI KDIELQGIKR QPGVWDYKDS NYSVLAYIIA EVSGEPYEQY
     IKNHIFKPAG MTHAGFYKTY EKEPYPAVGY KMEGSKTVTP YIPDLSQLYG AGDIYMSAID
     MYKFDQALID GKLYSQKSYE KMFTPGSSST YGMGFYVAPG SYSNHGVMPG FNILNSFSKS
     GQTIVILFSN IQNNAKLGQV NNKIYQLLNQ E