ID   PBUE_BACAM              Reviewed;         386 AA.
AC   Q0GQS6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Purine efflux pump PbuE;
GN   Name=pbuE;
OS   Bacillus amyloliquefaciens (Bacillus velezensis).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS AN EFFLUX PUMP, AND
RP   INDUCTION.
RC   STRAIN=DSM 1061 / JCM 20197 / IAM 1523 / K;
RX   PubMed=17935948; DOI=10.1016/j.resmic.2007.08.003;
RA   Zakataeva N.P., Gronskiy S.V., Sheremet A.S., Kutukova E.A., Novikova A.E.,
RA   Livshits V.A.;
RT   "A new function for the Bacillus PbuE purine base efflux pump: efflux of
RT   purine nucleosides.";
RL   Res. Microbiol. 158:659-665(2007).
CC   -!- FUNCTION: Involved in the efflux of purine ribonucleosides, such as
CC       guanosine, adenosine and inosine, as well as purine bases guanine,
CC       adenine and hypoxanthine, and purine base analogs 2,6-diaminopurine and
CC       6-mercaptopurine. Therefore plays a role in maintaining the cellular
CC       purine base pools and protecting cells against toxic purine base
CC       analogs. Modulates expression of the purR and G-box regulons (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:17935948}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Up-regulated by adenine, via the adenine-dependent
CC       riboswitch. {ECO:0000269|PubMed:17935948}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family.
CC       PbuE (TC 2.A.1.2.25) subfamily. {ECO:0000305}.
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DR   EMBL; DQ662404; ABG49512.1; -; Genomic_DNA.
DR   RefSeq; WP_013351164.1; NZ_VRTX01000015.1.
DR   AlphaFoldDB; Q0GQS6; -.
DR   SMR; Q0GQS6; -.
DR   STRING; 692420.BAMF_0515; -.
DR   eggNOG; COG2814; Bacteria.
DR   OMA; SAHFTAY; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..386
FT                   /note="Purine efflux pump PbuE"
FT                   /id="PRO_0000367244"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        235..255
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        262..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        328..348
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   386 AA;  41014 MW;  462F65B8A293B0F7 CRC64;
     MNFKVFLLAA STIAVGLVEL IVGGILPQIA SDLDISIVSA GQLISVFALG YAVSGPLLLA
     VTAKAERKRL YLIALFVFFL SNLVAYFSPN FAVLMVSRVL ASMSTGLIVV LSLTIAPKIV
     APEYRARAIG IIFMGFSSAI ALGVPVGIII SNAFGWRVLF LGIGVLSLVS MLIISVFFEK
     IPAEKMIPFR EQIKTIGNAK IASAHLVTLF TLAGHYTLYA YFAPFLETTL HLSSVWVSVC
     YFLFGLSAVC GGPFGGWLYD RLGSFKSIML VTVSFALILF ILPLSTVSLI VFLPAMVIWG
     LLSWSLAPAQ QSYLIKIAPE SSDIQQSFNT SALQIGIALG SAIGGGVIGQ TGSVTATAWC
     GGLIVIIAVS LAVFSLTRPA LKRKSA