ID   PBUO_BACSU              Reviewed;         432 AA.
AC   O34978; Q795S2;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Guanine/hypoxanthine permease PbuO;
GN   Name=pbuO; Synonyms=ytiP; OrderedLocusNames=BSU29990;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION IN PURINE UPTAKE, AND INDUCTION.
RC   STRAIN=168;
RX   PubMed=11591660; DOI=10.1128/jb.183.21.6175-6183.2001;
RA   Saxild H.H., Brunstedt K., Nielsen K.I., Jarmer H., Nygaard P.;
RT   "Definition of the Bacillus subtilis PurR operator using genetic and
RT   bioinformatic tools and expansion of the PurR regulon with glyA, guaC,
RT   pbuG, xpt-pbuX, yqhZ-folD, and pbuO.";
RL   J. Bacteriol. 183:6175-6183(2001).
CC   -!- FUNCTION: Involved in the uptake of the purine bases hypoxanthine and
CC       guanine. May work at purine concentrations higher than 100 uM.
CC       {ECO:0000269|PubMed:11591660}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Expression is regulated by the purR regulon. Down-regulated
CC       by adenine and up-regulated by guanosine.
CC       {ECO:0000269|PubMed:11591660}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. Azg-like subfamily. {ECO:0000305}.
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DR   EMBL; AF008220; AAC00391.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14977.1; -; Genomic_DNA.
DR   PIR; G69993; G69993.
DR   RefSeq; NP_390877.1; NC_000964.3.
DR   RefSeq; WP_003229234.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O34978; -.
DR   STRING; 224308.BSU29990; -.
DR   PaxDb; O34978; -.
DR   EnsemblBacteria; CAB14977; CAB14977; BSU_29990.
DR   GeneID; 938070; -.
DR   KEGG; bsu:BSU29990; -.
DR   PATRIC; fig|224308.179.peg.3257; -.
DR   eggNOG; COG2252; Bacteria.
DR   InParanoid; O34978; -.
DR   OMA; RKGSYFF; -.
DR   PhylomeDB; O34978; -.
DR   BioCyc; BSUB:BSU29990-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; IBA:GO_Central.
DR   InterPro; IPR045018; Azg-like.
DR   InterPro; IPR026033; Azg-like_bact_archaea.
DR   InterPro; IPR006043; NCS2.
DR   PANTHER; PTHR43337; PTHR43337; 1.
DR   Pfam; PF00860; Xan_ur_permease; 1.
DR   PIRSF; PIRSF005353; PbuG; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..432
FT                   /note="Guanine/hypoxanthine permease PbuO"
FT                   /id="PRO_0000375869"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        340..360
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   432 AA;  45420 MW;  727708F7371818C3 CRC64;
     MFHLKEQQTS IKQEIIAGLT TFFTMVYIVV VNPVILANAG VPFDQVFTAT IIASIVGTLW
     MALAANYPIA IAPGMGLNAY LAFHVVSASD GGITYATAFS AVFTAGVLFI ILSLTPLRKQ
     LIEAIPNNLK YGITTGIGLF IAFIGLRQAG IVAADESNLV TLGNLHSPGV ILTLVGLLIS
     VVLMVLNVSG ALFIGMAATA LIAFFTGQLH FSKGFMSLPH LPEGLMISNP FTAFGDVIHH
     GLYAVVFSFL LVTIFDTTGT MIGVAEQAGL MKNNKLPNVR KALLADSTAT TVGAVFGTSP
     TTAFIESSAG VAAGGRTGLT ALTVAVMFAA SMFFSPLVSA LSGIAAITSP ALIIVGSLMM
     GSVSNMNWKE MDEAFPAFLV ILAMPLTSSI STGIALGFIS YPIVKAARGK WREIHPLVIV
     FAILFFIQLF IL