ID   PBURS_APIME             Reviewed;         145 AA.
AC   A2VB90; Q32TG6; Q566B0;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Partner of bursicon;
DE   AltName: Full=Bursicon subunit beta;
DE   AltName: Full=Single-chain bursicon;
DE   Flags: Precursor;
GN   Name=pburs; Synonyms=sc-burs;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15703293; DOI=10.1073/pnas.0409916102;
RA   Luo C.-W., Dewey E.M., Sudo S., Ewer J., Hsu S.Y., Honegger H.-W.,
RA   Hsueh A.J.W.;
RT   "Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine
RT   knot protein that activates G protein-coupled receptor LGR2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:2820-2825(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Larva;
RX   PubMed=17275819; DOI=10.1016/j.ygcen.2006.12.004;
RA   Van Loy T., Van Hiel M.B., Vandersmissen H.P., Poels J., Mendive F.M.,
RA   Vassart G., Vanden Broeck J.J.M.;
RT   "Evolutionary conservation of bursicon in the animal kingdom.";
RL   Gen. Comp. Endocrinol. 153:59-63(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DH4;
RG   Honey bee genome project;
RA   Zhang L., Deng J., Wu Y.-Q., Kovar C., Aqrawi P., Bandaranaike D.,
RA   Blankenburg K., Chen D., Denson S., Dinh H., Firestine M., Gross S.,
RA   Han Y., Hernandez B., Holder M., Jackson L., Javaid M., Jing C., Jones J.,
RA   Joshi V., Kamau G., Korchina V., Lee S., Lorensuhewa L., Mata R.,
RA   Mathew T., Mims S., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Osuji N.,
RA   Pham C., Puazo M., Qu C., Quiroz J., Raj R., Rio Deiros D., Santibanez J.,
RA   Scheel M., Scherer S., Vee V., Wang M., Xin Y., Richards S., Reid J.G.,
RA   Newsham I., Worley K.C., Muzny D.M., Gibbs R.;
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=15811337; DOI=10.1016/j.febslet.2005.03.006;
RA   Mendive F.M., Van Loy T., Claeysen S., Poels J., Williamson M., Hauser F.,
RA   Grimmelikhuijzen C.J.P., Vassart G., Vanden Broeck J.J.M.;
RT   "Drosophila molting neurohormone bursicon is a heterodimer and the natural
RT   agonist of the orphan receptor DLGR2.";
RL   FEBS Lett. 579:2171-2176(2005).
CC   -!- FUNCTION: Final heterodimeric neurohormone released at the end of the
CC       molting cycle, involved in the sclerotization (tanning) of the insect
CC       cuticle, melanization and wing spreading.
CC       {ECO:0000269|PubMed:15811337}.
CC   -!- SUBUNIT: Heterodimer of burs and pburs. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAH89263.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY823258; AAX18443.1; -; mRNA.
DR   EMBL; AM420632; CAM06632.1; -; mRNA.
DR   EMBL; AADG06006208; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BN000692; CAH89263.2; ALT_SEQ; mRNA.
DR   RefSeq; NP_001035352.2; NM_001040262.2.
DR   AlphaFoldDB; A2VB90; -.
DR   STRING; 7460.GB45445-PA; -.
DR   PaxDb; A2VB90; -.
DR   EnsemblMetazoa; NM_001040262; NP_001035352; GeneID_413045.
DR   GeneID; 413045; -.
DR   KEGG; ame:413045; -.
DR   CTD; 34845; -.
DR   eggNOG; ENOG502S111; Eukaryota.
DR   HOGENOM; CLU_145016_0_0_1; -.
DR   InParanoid; A2VB90; -.
DR   OMA; SKECYCC; -.
DR   OrthoDB; 1461272at2759; -.
DR   PhylomeDB; A2VB90; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0031395; C:bursicon neuropeptide hormone complex; IEA:InterPro.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; IEA:InterPro.
DR   GO; GO:0005184; F:neuropeptide hormone activity; IEA:InterPro.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR034441; Bursicon_suB.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   PANTHER; PTHR41151; PTHR41151; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..145
FT                   /note="Partner of bursicon"
FT                   /id="PRO_0000223892"
FT   DOMAIN          36..131
FT                   /note="CTCK"
FT   DISULFID        36..94
FT                   /evidence="ECO:0000250"
FT   DISULFID        60..109
FT                   /evidence="ECO:0000250"
FT   DISULFID        69..135
FT                   /evidence="ECO:0000250"
FT   DISULFID        73..137
FT                   /evidence="ECO:0000250"
FT   DISULFID        91..140
FT                   /evidence="ECO:0000250"
FT   DISULFID        93
FT                   /note="Interchain"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   145 AA;  16608 MW;  1A2D65A2F8A05272 CRC64;
     MKENFSIMFI HSIFLILIIF IYSNETIAQV TDDENCETLQ SEVHITKDEY DEIGRLKRTC
     SGDISVTKCE GFCNSQVQPS VASTTGFSKE CYCCRESYLK ERHITLHHCY DADGIKLMNE
     ENGVMEIKIR EPVECKCIKC GDISQ