PBX1_XENLA
ID PBX1_XENLA Reviewed; 347 AA.
AC Q8QGC4;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Pre-B-cell leukemia transcription factor 1 {ECO:0000250|UniProtKB:P40424};
DE Short=Xpbx1 {ECO:0000303|PubMed:11960001};
DE AltName: Full=Homeobox protein pbx1 {ECO:0000250|UniProtKB:P40424};
DE AltName: Full=Pre-B-cell leukemia transcription factor 1b {ECO:0000312|EMBL:AAM18914.1};
DE Short=Xpbx1b {ECO:0000303|PubMed:11960001};
GN Name=pbx1; Synonyms=pbx1b {ECO:0000312|EMBL:AAM18914.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAM18914.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MEIS1, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryonic head {ECO:0000269|PubMed:11960001};
RX PubMed=11960001; DOI=10.1073/pnas.082654899;
RA Maeda R., Ishimura A., Mood K., Park E.K., Buchberg A.M., Daar I.O.;
RT "Xpbx1b and Xmeis1b play a collaborative role in hindbrain and neural crest
RT gene expression in Xenopus embryos.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:5448-5453(2002).
RN [2] {ECO:0000312|EMBL:AAI06423.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula {ECO:0000312|EMBL:AAI06423.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=16631621; DOI=10.1016/j.bbrc.2006.03.235;
RA Kelly L.E., Carrel T.L., Herman G.E., El-Hodiri H.M.;
RT "Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter
RT through a highly conserved region.";
RL Biochem. Biophys. Res. Commun. 344:1031-1037(2006).
CC -!- FUNCTION: Acts as a transcriptional activator in complex with isoform 2
CC of meis1, to induce posterior neural and neural crest gene expression,
CC and thereby specify hindbrain and neural crest cell fate. Binds to a
CC highly conserved region in the promoter of the neural crest gene zic3.
CC Required for the nuclear transport or retention of isoform 2 of meis1.
CC {ECO:0000269|PubMed:11960001, ECO:0000269|PubMed:16631621}.
CC -!- SUBUNIT: Forms a heterodimer with isoform 2 of meis1; the interaction
CC is necessary for neural fate induction. {ECO:0000269|PubMed:11960001}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P40424}.
CC -!- TISSUE SPECIFICITY: Shows broad, weak expression from blastula through
CC gastrula stages. At stage 14/15, expressed in a broad arc that gives
CC rise to the forebrain and eyes. More intensely expressed in the lateral
CC neural folds (presumptive neural crest) and as horizontal stripes in
CC the posterior neural plate that give rise to the hindbrain. As
CC development proceeds, expression progresses posteriorly along the
CC neural folds and at stage 21, expression is pronounced in the
CC prospective hindbrain and in migratory neural crest cells. At later
CC stages (stage 26), expression becomes intense within the dorsal portion
CC of the forebrain, and in the optic cup, caudal branchial arch,
CC peripheral to the pronephric anlage, and in the dorsal anterior half of
CC the spinal cord. Expression remains robust in the hindbrain but
CC gradually becomes more restricted. At stage 28, expressed in the dorsal
CC lateral portion of the neural tube and in the somatic layer of the
CC lateral plate mesoderm that surrounds the pronephric anlage.
CC {ECO:0000269|PubMed:11960001}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expressed at a low level in unfertilized eggs with increased expression
CC during late gastrula through neurula and tailbud stages.
CC {ECO:0000269|PubMed:11960001}.
CC -!- MISCELLANEOUS: The displayed sequence corresponds to the ortholog of
CC the mammalian isoform B sequences. There is currently no X.laevis
CC ortholog sequence for isoform A. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TALE/PBX homeobox family. {ECO:0000255}.
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DR EMBL; AF480430; AAM18914.1; -; mRNA.
DR EMBL; BC106422; AAI06423.1; -; mRNA.
DR RefSeq; NP_001083906.1; NM_001090437.1.
DR AlphaFoldDB; Q8QGC4; -.
DR SMR; Q8QGC4; -.
DR DNASU; 399184; -.
DR GeneID; 399184; -.
DR KEGG; xla:399184; -.
DR CTD; 399184; -.
DR Xenbase; XB-GENE-1217596; pbx1.S.
DR OMA; FCIGHAN; -.
DR Proteomes; UP000186698; Chromosome 4S.
DR Bgee; 399184; Expressed in internal ear and 19 other tissues.
DR GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IGI:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:1904888; P:cranial skeletal system development; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0014036; P:neural crest cell fate specification; IGI:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR005542; PBX.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03792; PBC; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS51978; PBC; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..347
FT /note="Pre-B-cell leukemia transcription factor 1"
FT /id="PRO_0000365098"
FT DOMAIN 38..232
FT /note="PBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT DNA_BIND 233..295
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 45..124
FT /note="PBC-A"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT REGION 127..232
FT /note="PBC-B"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT REGION 318..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 347 AA; 38562 MW; 6931ABD2B86EFF6C CRC64;
MDDQPRLMHS HPGVGMAGHP SLSQHMQDGT GANEGDVGRK QDIGDILQQI MTITDQSLDE
AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP SDPQLMRLDN MLLAEGVAGP
EKGGGSAAAA AAAAASGGAG ADNSTEHSDY RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM
NLLREQSRTR PISPKEIERM VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF
NKQATEILNE YFYSHLSNPY PSEEAKEELA KKCAITVSQV SNWFGNKRIR YKKNIGKFQE
EANIYAAKTA VNATNVSVHG SQANSPSTPS SAGGYPSPCY QSDRRIQ
