PBX4_MOUSE
ID PBX4_MOUSE Reviewed; 378 AA.
AC Q99NE9; Q8CFL2; Q99NE8;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Pre-B-cell leukemia transcription factor 4;
DE AltName: Full=Homeobox protein PBX4;
GN Name=Pbx4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-296 (ISOFORMS 1 AND 2), AND TISSUE
RP SPECIFICITY.
RC STRAIN=FVB/N;
RX PubMed=11335119; DOI=10.1016/s0925-4773(01)00349-5;
RA Wagner K., Mincheva A., Korn B., Lichter P., Poepperl H.;
RT "Pbx4, a new Pbx family member on mouse chromosome 8 is expressed during
RT spermatogenesis.";
RL Mech. Dev. 103:127-131(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-378 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99NE9-1; Sequence=Displayed;
CC Name=2; Synonyms=Truncated;
CC IsoId=Q99NE9-2; Sequence=VSP_008617, VSP_008618;
CC -!- TISSUE SPECIFICITY: Almost exclusively expressed in testis.
CC {ECO:0000269|PubMed:11335119}.
CC -!- DEVELOPMENTAL STAGE: Expressed in spermatocytes in the pachytene stage
CC of the first meiotic prophase.
CC -!- SIMILARITY: Belongs to the TALE/PBX homeobox family. {ECO:0000305}.
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DR EMBL; AJ300183; CAC28213.1; -; mRNA.
DR EMBL; AJ300184; CAC28214.1; -; mRNA.
DR EMBL; AK010506; BAC25299.1; -; mRNA.
DR EMBL; BC032875; AAH32875.1; -; mRNA.
DR CCDS; CCDS22352.1; -. [Q99NE9-1]
DR RefSeq; NP_001020125.1; NM_001024954.1. [Q99NE9-1]
DR RefSeq; XP_006509865.1; XM_006509802.3.
DR RefSeq; XP_006509866.1; XM_006509803.1.
DR AlphaFoldDB; Q99NE9; -.
DR SMR; Q99NE9; -.
DR BioGRID; 219800; 9.
DR IntAct; Q99NE9; 9.
DR STRING; 10090.ENSMUSP00000080219; -.
DR PhosphoSitePlus; Q99NE9; -.
DR PaxDb; Q99NE9; -.
DR PRIDE; Q99NE9; -.
DR ProteomicsDB; 294024; -. [Q99NE9-1]
DR ProteomicsDB; 294025; -. [Q99NE9-2]
DR Antibodypedia; 28510; 90 antibodies from 24 providers.
DR DNASU; 80720; -.
DR Ensembl; ENSMUST00000081503; ENSMUSP00000080219; ENSMUSG00000031860. [Q99NE9-1]
DR Ensembl; ENSMUST00000132899; ENSMUSP00000118287; ENSMUSG00000031860. [Q99NE9-2]
DR GeneID; 80720; -.
DR KEGG; mmu:80720; -.
DR UCSC; uc009lxx.1; mouse. [Q99NE9-1]
DR CTD; 80714; -.
DR MGI; MGI:1931321; Pbx4.
DR VEuPathDB; HostDB:ENSMUSG00000031860; -.
DR eggNOG; KOG0774; Eukaryota.
DR GeneTree; ENSGT00940000162643; -.
DR HOGENOM; CLU_041153_0_1_1; -.
DR InParanoid; Q99NE9; -.
DR OMA; IYMGKTA; -.
DR OrthoDB; 804740at2759; -.
DR PhylomeDB; Q99NE9; -.
DR TreeFam; TF314340; -.
DR BioGRID-ORCS; 80720; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Pbx4; mouse.
DR PRO; PR:Q99NE9; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q99NE9; protein.
DR Bgee; ENSMUSG00000031860; Expressed in spermatocyte and 89 other tissues.
DR ExpressionAtlas; Q99NE9; baseline and differential.
DR Genevisible; Q99NE9; MM.
DR GO; GO:0005634; C:nucleus; IC:BHF-UCL.
DR GO; GO:0001741; C:XY body; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:BHF-UCL.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IBA:GO_Central.
DR GO; GO:0048568; P:embryonic organ development; IBA:GO_Central.
DR GO; GO:0001654; P:eye development; IBA:GO_Central.
DR GO; GO:0048666; P:neuron development; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IC:BHF-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR005542; PBX.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03792; PBC; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS51978; PBC; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..378
FT /note="Pre-B-cell leukemia transcription factor 4"
FT /id="PRO_0000049242"
FT DOMAIN 22..214
FT /note="PBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT DNA_BIND 215..277
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 29..107
FT /note="PBC-A"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT REGION 100..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..214
FT /note="PBC-B"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT REGION 291..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 355..378
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 73..74
FT /note="AV -> GL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11335119,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_008617"
FT VAR_SEQ 75..378
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11335119,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_008618"
SQ SEQUENCE 378 AA; 41961 MW; E3A8DEA28E428AA0 CRC64;
MAAPLRPVPP QPAPRRLPTT APLGHDTSDV LQQIMAITDQ SLDEAQARKH ALNCHRMKSA
LFSVLCEIKG KTAVSIQFQE EDPPDAQLLR LDNMLLAEGV SRPEKRGRGA AAGSTATPGG
CPNDNSIEHS DYRAKLSQIR QIYHSELEKY EQACREFTTH VTNLLREQSR VRPVSCREME
HMVNTIQSKF SAIQRQLKQS TCEAVMTLRS RFLDARRKRR NFSKQATDVL NEYFYSHLSN
PYPSEETKEE LARKGGITVS QVSNWFGNKR IRYKKNTGKF QEEATMYTGK ASTVTKARRP
RGQSSCQSTP SPGPCGPLPL TNGSDVVLTL RTLAFLQPPT GGVCLQPLVH SNWQRAAPQP
ASSPAGESGS FNWDAASN
