PC14_PENCR
ID PC14_PENCR Reviewed; 577 AA.
AC A0A0E3D8M4;
DT 10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT 24-JUN-2015, sequence version 1.
DT 25-MAY-2022, entry version 15.
DE RecName: Full=Monooxygenase PC-14 {ECO:0000303|PubMed:26213965};
DE EC=1.-.-.- {ECO:0000305|PubMed:26213965};
DE AltName: Full=Penitrem biosynthesis cluster 1 protein PC-14 {ECO:0000303|PubMed:26213965};
GN Name=PC-14 {ECO:0000303|PubMed:26213965};
OS Penicillium crustosum (Blue mold fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=36656;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, FUNCTION, AND PATHWAY.
RC STRAIN=PN2402;
RX PubMed=26213965; DOI=10.3390/toxins7082701;
RA Nicholson M.J., Eaton C.J., Starkel C., Tapper B.A., Cox M.P., Scott B.;
RT "Molecular cloning and functional analysis of gene clusters for the
RT biosynthesis of indole-diterpenes in Penicillium crustosum and P.
RT janthinellum.";
RL Toxins 7:2701-2722(2015).
CC -!- FUNCTION: Monooxygenase; part of the gene cluster that mediates the
CC biosynthesis of the indole diterpenes penitrems (PubMed:26213965). The
CC geranylgeranyl diphosphate (GGPP) synthase penG catalyzes the first
CC step in penitrem biosynthesis via conversion of farnesyl pyrophosphate
CC and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP)
CC (Probable). Condensation of indole-3-glycerol phosphate with GGPP by
CC the prenyl transferase penC then forms 3-geranylgeranylindole (3-GGI)
CC (Probable). Epoxidation by the FAD-dependent monooxygenase penM leads
CC to a epoxidized-GGI that is substrate of the terpene cyclase penB for
CC cyclization to yield paspaline (Probable). Paspaline is subsequently
CC converted to 13-desoxypaxilline by the cytochrome P450 monooxygenase
CC penP, the latter being then converted to paxilline by the cytochrome
CC P450 monooxygenase penQ (PubMed:26213965). Paxilline is converted to
CC beta-paxitriol via C-10 ketoreduction by the short-chain dehydrogenase
CC PC-15 which can be monoprenylated at the C-20 by the indole diterpene
CC prenyltransferase penD (Probable). A two-step elimination (acetylation
CC and elimination) process performed by the O-acetyltransferase PC-16 and
CC the P.simplicissimum ptmI-ortholog not yet identified in P.crustosum,
CC leads to the production of the prenylated form of penijanthine
CC (Probable). The FAD-linked oxidoreductase ptmO then converts the
CC prenylated form of penijanthine into PC-M5 which is in turn transformed
CC into PC-M4 by the aromatic dimethylallyltransferase PC-22 (Probable). A
CC series of oxidation steps involving 4 cytochrome P450 monooxygenases
CC (PC-21, PC-05, PC-23, PC-20) and a FAD-dependent monooxygenase (PC-14)
CC are required for the transformation of PC-M4 to penitrems A and E.
CC Synthesis of these final products is proposed to proceed via penitrems
CC D and C (PC-21, PC-05, PC-14) and penitrems B and F (PC-21, PC-05, PC-
CC 14, PC-23) (Probable). {ECO:0000269|PubMed:26213965,
CC ECO:0000305|PubMed:26213965}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:26213965}.
CC -!- SIMILARITY: Belongs to the FMO family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KC963408; AGZ20195.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0E3D8M4; -.
DR SMR; A0A0E3D8M4; -.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0004499; F:N,N-dimethylaniline monooxygenase activity; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000960; Flavin_mOase.
DR InterPro; IPR020946; Flavin_mOase-like.
DR Pfam; PF00743; FMO-like; 2.
DR PIRSF; PIRSF000332; FMO; 1.
DR PRINTS; PR00370; FMOXYGENASE.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 3: Inferred from homology;
KW FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase.
FT CHAIN 1..577
FT /note="Monooxygenase PC-14"
FT /id="PRO_0000446590"
SQ SEQUENCE 577 AA; 64467 MW; 2E76196FA851F2D0 CRC64;
MKVAVIGGGP SGLVTLKYLL AAHHFQPVDP IEVQLFESED RVGGTFSYRT YDRAELVSSA
QLTTFSDYRW HDKSVDYLSA VEYVEYLEGY CDRFGLRPHI RLSTQVEKVE RTGKGKHRIT
VSHKGQTSTW DCDAVAVCSG LHVKPNIPSI PGLDRVPVVF HSSEYKHVRQ LGQNTNVMVL
GTGETGMDIA YFSVTADSTK STTVCHRNGF VIGPKRLPET KLFGRVTSKT PGKALPVDLS
RPYLFVNSYV HRKVRGALQT TLSRWAVKAG SWLVTGTTRG FDQWVGSLPK DKYDESHYFY
CKSTKAMPYI SAPYRSHSWV HRLRSSIVQA VLPDTGSRKI DLAPWPEYID EDGVVHFEKN
SRPDSKVLLQ ERRFKPDVLV LATGYTQSFP FLGSEYCTPD QADQRGIWRT GDASVGYIGF
VRPSFGAIPP LAEMQVQVWV LNLINRLPGP LVADDSYRLF SNPSGRIEYG VDHDMFAHRL
ALDIGSAPSF FQALAHGWQV IVFWAMGGTL NTKFRLVGPW AWSGAPRIIH DELLDTVTGR
RSTIDLLVAL CIRIFQATSV VSSRPSKGDS EVTENRG