ID   PCAB_BRADU              Reviewed;         451 AA.
AC   O31385;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=3-carboxy-cis,cis-muconate cycloisomerase;
DE            EC=5.5.1.2;
DE   AltName: Full=3-carboxymuconate lactonizing enzyme;
DE            Short=CMLE;
GN   Name=pcaB; OrderedLocusNames=blr5667;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=9582432; DOI=10.1016/s0167-4781(98)00048-7;
RA   Lorite M.J., Sanjuan J., Velasco L., Olivares J., Bedmar E.J.;
RT   "Characterization of Bradyrhizobium japonicum pcaBDC genes involved in 4-
RT   hydroxybenzoate degradation.";
RL   Biochim. Biophys. Acta 1397:257-261(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Catalyzes an anti cycloisomerization.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(carboxymethyl)-5-oxo-2,5-dihydro-2-furoate = 3-carboxy-
CC         cis,cis-muconate + H(+); Xref=Rhea:RHEA:23656, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57496, ChEBI:CHEBI:57979; EC=5.5.1.2;
CC   -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family.
CC       {ECO:0000305}.
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DR   EMBL; Y10223; CAA71270.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC50932.1; -; Genomic_DNA.
DR   RefSeq; NP_772307.1; NC_004463.1.
DR   RefSeq; WP_011088413.1; NC_004463.1.
DR   AlphaFoldDB; O31385; -.
DR   SMR; O31385; -.
DR   STRING; 224911.27353943; -.
DR   EnsemblBacteria; BAC50932; BAC50932; BAC50932.
DR   GeneID; 64025437; -.
DR   KEGG; bja:blr5667; -.
DR   PATRIC; fig|224911.5.peg.5776; -.
DR   eggNOG; COG0015; Bacteria.
DR   HOGENOM; CLU_030949_3_3_5; -.
DR   InParanoid; O31385; -.
DR   OMA; HTQRDEW; -.
DR   PhylomeDB; O31385; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0047472; F:3-carboxy-cis,cis-muconate cycloisomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016840; F:carbon-nitrogen lyase activity; IEA:UniProt.
DR   GO; GO:0019619; P:3,4-dihydroxybenzoate catabolic process; IEA:InterPro.
DR   InterPro; IPR019468; AdenyloSucc_lyase_C.
DR   InterPro; IPR020557; Fumarate_lyase_CS.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   InterPro; IPR012789; Protocat_PcaB.
DR   Pfam; PF10397; ADSL_C; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SMART; SM00998; ADSL_C; 1.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR02426; protocat_pcaB; 1.
DR   PROSITE; PS00163; FUMARATE_LYASES; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Isomerase; Reference proteome.
FT   CHAIN           1..451
FT                   /note="3-carboxy-cis,cis-muconate cycloisomerase"
FT                   /id="PRO_0000161348"
SQ   SEQUENCE   451 AA;  47238 MW;  DB6220DA9C5E1981 CRC64;
     MSTALSPLLA PMLSSAAMRA ACDDRSTLQN MLDFEAALAR AEAATGVIPA AAVGPIETAC
     KAATFDIAAL AEAATRSGNL AIPLVKALTA NVAKADAEAA RYVHWGATSQ DVIDTATMLT
     LRAAIDALDA DLSRAIKGFA ALARNHRNTA MVARTWLQHA LPMPFGLKAA EYAASLARAR
     CRLRRLSRDG LALQFGGAAG TLAALGDKGL VVAERLAQEL NLPLPEAPWH THRDRIAEAA
     SALAILAGSC GKIARDVSLM MQTDVAEAFE PAGEGRGGSS TMPHKRNPVA AASALGCATM
     APQLAATIFA AQVQDHERSA GPWHAEWPTL PQLMLVTSGA LAAIVDIAEG LDVDAARMRS
     NLDATHGLIM AEAVTFALAE RIGKSDAHHL IEAASKRAVA EKKHLREVLA ADSQVTAHLS
     PEKIAALFEP MAYQGASQAM IDRLLDSLER D