ID   PCAB_PSEAE              Reviewed;         459 AA.
AC   Q9I6Q8;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=3-carboxy-cis,cis-muconate cycloisomerase;
DE            EC=5.5.1.2;
DE   AltName: Full=3-carboxymuconate lactonizing enzyme;
DE            Short=CMLE;
GN   Name=pcaB; OrderedLocusNames=PA0230;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes an anti cycloisomerization. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(carboxymethyl)-5-oxo-2,5-dihydro-2-furoate = 3-carboxy-
CC         cis,cis-muconate + H(+); Xref=Rhea:RHEA:23656, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57496, ChEBI:CHEBI:57979; EC=5.5.1.2;
CC   -!- PATHWAY: Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-
CC       4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate: step 1/2.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG03619.1; -; Genomic_DNA.
DR   PIR; B83616; B83616.
DR   RefSeq; NP_248921.1; NC_002516.2.
DR   RefSeq; WP_003112675.1; NZ_QZGE01000024.1.
DR   AlphaFoldDB; Q9I6Q8; -.
DR   SMR; Q9I6Q8; -.
DR   STRING; 287.DR97_3187; -.
DR   PaxDb; Q9I6Q8; -.
DR   PRIDE; Q9I6Q8; -.
DR   EnsemblBacteria; AAG03619; AAG03619; PA0230.
DR   GeneID; 881957; -.
DR   KEGG; pae:PA0230; -.
DR   PATRIC; fig|208964.12.peg.240; -.
DR   PseudoCAP; PA0230; -.
DR   HOGENOM; CLU_030949_3_3_6; -.
DR   InParanoid; Q9I6Q8; -.
DR   OMA; HRATPMI; -.
DR   PhylomeDB; Q9I6Q8; -.
DR   BioCyc; PAER208964:G1FZ6-232-MON; -.
DR   UniPathway; UPA00157; UER00265.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047472; F:3-carboxy-cis,cis-muconate cycloisomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016840; F:carbon-nitrogen lyase activity; IEA:UniProt.
DR   GO; GO:0019619; P:3,4-dihydroxybenzoate catabolic process; IEA:InterPro.
DR   GO; GO:0042952; P:beta-ketoadipate pathway; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR019468; AdenyloSucc_lyase_C.
DR   InterPro; IPR020557; Fumarate_lyase_CS.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   InterPro; IPR012789; Protocat_PcaB.
DR   Pfam; PF10397; ADSL_C; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SMART; SM00998; ADSL_C; 1.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR02426; protocat_pcaB; 1.
DR   PROSITE; PS00163; FUMARATE_LYASES; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Cytoplasm; Isomerase; Reference proteome.
FT   CHAIN           1..459
FT                   /note="3-carboxy-cis,cis-muconate cycloisomerase"
FT                   /id="PRO_0000287772"
SQ   SEQUENCE   459 AA;  49121 MW;  C43F6B3651F2D1D0 CRC64;
     MSDVPANQLF DAYFMAAPMR AVFSDRGRLQ GMLDFEAALA RAEARTGVVP ATAVAPIEAA
     CRAELYDPLA LAEAVATAGN SAIPLVKALG RQVAAGDAEA ERYVHLGATS QDAMDSGLVL
     QLRRALALLE QDLQRLAEVL ADQAERHADT PLAGRTWLQH ATPVTLGMKL AGLLGALTRH
     RQRLRELRPR LLVLQFGGAS GTLAALGEQA LPVAAALAEE LGLALPEQPW HTQRDRLVEF
     ASVLGLVAGS LGKFGRDVSL LMQTEAGEVF EPAGAGRGGS STMPHKRNPV SSAVLIAAAT
     RAPGLVSTLF AAMPQEHERS LGLWHAEWET LPELCCLVAG ALQQAIGLLE GLEVDAQRMR
     RNLGLTHGLV LAEAVSIALA RRIGREAAHH LVEQCCRRAV EQRRELRAVL GEEARVSAEL
     SGDELDRLLD PAHYLGQARA WVERALAEHH ALGFEPHPA