PCAK_PSEAE
ID PCAK_PSEAE Reviewed; 448 AA.
AC Q9I6Q3;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=4-hydroxybenzoate transporter PcaK;
GN Name=pcaK; OrderedLocusNames=PA0235;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Transports 4-hydroxybenzoate (4-HBA) and protocatechuate
CC across the membrane. Driven by the proton motive force. Functions also
CC as a chemoreceptor, which is required for chemotaxis to aromatic acids
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Aromatic
CC acid:H(+) symporter (AAHS) (TC 2.A.1.15) family. {ECO:0000305}.
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DR EMBL; AE004091; AAG03624.1; -; Genomic_DNA.
DR PIR; G83616; G83616.
DR RefSeq; NP_248926.1; NC_002516.2.
DR RefSeq; WP_003101875.1; NZ_QZGE01000024.1.
DR AlphaFoldDB; Q9I6Q3; -.
DR SMR; Q9I6Q3; -.
DR STRING; 287.DR97_3192; -.
DR PaxDb; Q9I6Q3; -.
DR EnsemblBacteria; AAG03624; AAG03624; PA0235.
DR GeneID; 881907; -.
DR KEGG; pae:PA0235; -.
DR PATRIC; fig|208964.12.peg.245; -.
DR PseudoCAP; PA0235; -.
DR HOGENOM; CLU_001265_46_4_6; -.
DR InParanoid; Q9I6Q3; -.
DR OMA; AGMCVNG; -.
DR PhylomeDB; Q9I6Q3; -.
DR BioCyc; PAER208964:G1FZ6-237-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR004746; MFS_AAHS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00895; 2A0115; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Chemotaxis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..448
FT /note="4-hydroxybenzoate transporter PcaK"
FT /id="PRO_0000287817"
FT TOPO_DOM 1..30
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..67
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..119
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..152
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..184
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..301
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..329
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 420..421
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 448 AA; 47588 MW; 7B34DD278828702B CRC64;
MNSPSLPAVE RLDVQAFINA QPLSPYQWRI VLLCFLIVFL DGLDTAAMGF IAPALTQDWG
IDRASLGPVM SAALIGMVFG ALGSGPLADR YGRKLVLVAA VFLFGLFSLA SAYSTNVEQL
LALRFLTGLG LGAAMPNATT LLSEYTPERL KSLLVTSMFC GFNLGMACGG FVSAKLIPLF
GWHSLLLLGG LLPLVLAVVL LFRLPESARY LVVRNRGSER VRQVLAPIAP AQVALARSFH
VPEQQTVQAR NVFAVIFSGT YSAGTLLLWL TYFMGLVIVY LLTSWLPTLM RDSGASLEQA
AFIGALFQFG GVLSAVAVGW AMDRFNPHKV IGLFYLLAGV FAWCVGQSLG QVTLLATLVL
LAGMCINGAQ SAMPSLAARF YPTQGRATGV SWMLGIGRFG AILGAWIGAT LLGLGWNFEQ
VLTALVLPAA LATAAVLLKG LVSHADAG
