ASPDH_HUMAN
ID ASPDH_HUMAN Reviewed; 283 AA.
AC A6ND91; Q6NZ37;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Aspartate dehydrogenase domain-containing protein {ECO:0000305};
GN Name=ASPDH {ECO:0000312|HGNC:HGNC:33856};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-266.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ARG-266.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND SER-168, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A6ND91-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A6ND91-2; Sequence=VSP_038742, VSP_038743;
CC -!- SIMILARITY: Belongs to the L-aspartate dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; CH471135; EAW71880.1; -; Genomic_DNA.
DR EMBL; AC008743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC066359; AAH66359.1; -; mRNA.
DR CCDS; CCDS33082.1; -. [A6ND91-2]
DR CCDS; CCDS46153.1; -. [A6ND91-1]
DR RefSeq; NP_001019827.2; NM_001024656.2. [A6ND91-2]
DR RefSeq; NP_001108070.1; NM_001114598.1. [A6ND91-1]
DR AlphaFoldDB; A6ND91; -.
DR SMR; A6ND91; -.
DR BioGRID; 299841; 15.
DR IntAct; A6ND91; 2.
DR MINT; A6ND91; -.
DR STRING; 9606.ENSP00000373860; -.
DR iPTMnet; A6ND91; -.
DR PhosphoSitePlus; A6ND91; -.
DR BioMuta; ASPDH; -.
DR MassIVE; A6ND91; -.
DR PaxDb; A6ND91; -.
DR PeptideAtlas; A6ND91; -.
DR PRIDE; A6ND91; -.
DR ProteomicsDB; 891; -. [A6ND91-1]
DR ProteomicsDB; 892; -. [A6ND91-2]
DR Antibodypedia; 46022; 77 antibodies from 12 providers.
DR DNASU; 554235; -.
DR Ensembl; ENST00000376916.7; ENSP00000366114.2; ENSG00000204653.10. [A6ND91-2]
DR Ensembl; ENST00000389208.9; ENSP00000373860.3; ENSG00000204653.10. [A6ND91-1]
DR GeneID; 554235; -.
DR KEGG; hsa:554235; -.
DR MANE-Select; ENST00000389208.9; ENSP00000373860.3; NM_001114598.2; NP_001108070.1.
DR UCSC; uc002psr.4; human. [A6ND91-1]
DR CTD; 554235; -.
DR GeneCards; ASPDH; -.
DR HGNC; HGNC:33856; ASPDH.
DR HPA; ENSG00000204653; Tissue enhanced (brain, kidney, liver).
DR neXtProt; NX_A6ND91; -.
DR OpenTargets; ENSG00000204653; -.
DR PharmGKB; PA164716234; -.
DR VEuPathDB; HostDB:ENSG00000204653; -.
DR eggNOG; ENOG502QVGC; Eukaryota.
DR GeneTree; ENSGT00390000004452; -.
DR HOGENOM; CLU_063528_0_0_1; -.
DR InParanoid; A6ND91; -.
DR OMA; HIVEVDV; -.
DR OrthoDB; 1182611at2759; -.
DR PhylomeDB; A6ND91; -.
DR TreeFam; TF315092; -.
DR PathwayCommons; A6ND91; -.
DR SignaLink; A6ND91; -.
DR BioGRID-ORCS; 554235; 19 hits in 1067 CRISPR screens.
DR ChiTaRS; ASPDH; human.
DR GenomeRNAi; 554235; -.
DR Pharos; A6ND91; Tbio.
DR PRO; PR:A6ND91; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; A6ND91; protein.
DR Bgee; ENSG00000204653; Expressed in right lobe of liver and 144 other tissues.
DR ExpressionAtlas; A6ND91; baseline and differential.
DR Genevisible; A6ND91; HS.
DR GO; GO:0033735; F:aspartate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0009435; P:NAD biosynthetic process; IEA:InterPro.
DR InterPro; IPR005106; Asp/hSer_DH_NAD-bd.
DR InterPro; IPR002811; Asp_DH.
DR InterPro; IPR011182; L-Asp_DH.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01958; DUF108; 1.
DR Pfam; PF03447; NAD_binding_3; 1.
DR PIRSF; PIRSF005227; Asp_dh_NAD_syn; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..283
FT /note="Aspartate dehydrogenase domain-containing protein"
FT /id="PRO_0000300623"
FT MOD_RES 20
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 2..65
FT /note="ADRGPWRVGVVGYGRLGQSLVSRLLAQGPELGLELVFVWNRDPGRMAGSVPP
FT SLQLQNLAALGE -> GDRVKGSKS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_038742"
FT VAR_SEQ 95..144
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_038743"
FT VARIANT 266
FT /note="Q -> R (in dbSNP:rs12977172)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT /id="VAR_062676"
SQ SEQUENCE 283 AA; 29946 MW; FF4254EF3F14D840 CRC64;
MADRGPWRVG VVGYGRLGQS LVSRLLAQGP ELGLELVFVW NRDPGRMAGS VPPSLQLQNL
AALGERRPDL VVEVAHPKII HESGAQILRH ANLLVGSPSA LSDQTTERQL LEASQHWDHA
VFVARGALWG AEDIRRLDAA GGLRSLRVTM ATHPDGFRLE GPLAAAHSPG PCTVLYEGPV
RGLCPFAPRN SNTMAAAALA APSLGFDGVI GVLVADTSLT DMHVVDVELS GPRGPTGRSF
AVHTRRENPA EPGAVTGSAT VTAFWQSLLA CCQLPSRPGI HLC
