PCBP3_HUMAN
ID PCBP3_HUMAN Reviewed; 371 AA.
AC P57721; A8MPS2; A8MQ26; B7WNN9; B7WPC1; Q8N9K6; Q96EP6;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Poly(rC)-binding protein 3;
DE AltName: Full=Alpha-CP3;
DE AltName: Full=PCBP3-overlapping transcript {ECO:0000312|HGNC:HGNC:8651};
DE AltName: Full=PCBP3-overlapping transcript 1 {ECO:0000312|HGNC:HGNC:8651};
GN Name=PCBP3 {ECO:0000312|HGNC:HGNC:8651};
GN Synonyms=PCBP3-OT1 {ECO:0000312|HGNC:HGNC:8651},
GN PCBP3OT {ECO:0000312|HGNC:HGNC:8651};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=10936052; DOI=10.1006/geno.2000.6244;
RA Makeyev A.V., Liebhaber S.A.;
RT "Identification of two novel mammalian genes establishes a subfamily of KH-
RT domain RNA-binding proteins.";
RL Genomics 67:301-316(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10830953; DOI=10.1038/35012518;
RA Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S.,
RA Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M.,
RA Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U.,
RA Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A.,
RA Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J.,
RA Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K.,
RA Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G.,
RA Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J.,
RA Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S.,
RA Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K.,
RA Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.;
RT "The DNA sequence of human chromosome 21.";
RL Nature 405:311-319(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=14612387; DOI=10.1128/mcb.23.23.8405-8415.2003;
RA Chkheidze A.N., Liebhaber S.A.;
RT "A novel set of nuclear localization signals determine distributions of the
RT alphaCP RNA-binding proteins.";
RL Mol. Cell. Biol. 23:8405-8415(2003).
CC -!- FUNCTION: Single-stranded nucleic acid binding protein that binds
CC preferentially to oligo dC. {ECO:0000250}.
CC -!- INTERACTION:
CC P57721-2; Q9BZC1-2: CELF4; NbExp=3; IntAct=EBI-11983983, EBI-12818201;
CC P57721-2; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-11983983, EBI-742054;
CC P57721-2; P43243: MATR3; NbExp=3; IntAct=EBI-11983983, EBI-352602;
CC P57721-2; Q9UHX1-2: PUF60; NbExp=3; IntAct=EBI-11983983, EBI-11529177;
CC P57721-2; A0AV96: RBM47; NbExp=3; IntAct=EBI-11983983, EBI-2823850;
CC P57721-2; P78317: RNF4; NbExp=3; IntAct=EBI-11983983, EBI-2340927;
CC P57721-2; P09012: SNRPA; NbExp=5; IntAct=EBI-11983983, EBI-607085;
CC P57721-2; P84103: SRSF3; NbExp=3; IntAct=EBI-11983983, EBI-372557;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14612387}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=P57721-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P57721-2; Sequence=VSP_009947, VSP_009948;
CC Name=3;
CC IsoId=P57721-3; Sequence=VSP_009947;
CC Name=4;
CC IsoId=P57721-4; Sequence=VSP_009948;
CC Name=5;
CC IsoId=P57721-5; Sequence=VSP_010014;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG09240.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH12061.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC04327.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF176329; AAG09240.1; ALT_INIT; mRNA.
DR EMBL; AK094301; BAC04327.2; ALT_INIT; mRNA.
DR EMBL; AJ011931; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJ239328; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL133492; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL133493; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL592528; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC012061; AAH12061.1; ALT_INIT; mRNA.
DR CCDS; CCDS42974.2; -. [P57721-1]
DR CCDS; CCDS46652.1; -. [P57721-2]
DR CCDS; CCDS86993.1; -. [P57721-5]
DR CCDS; CCDS86994.1; -. [P57721-4]
DR RefSeq; NP_001123613.1; NM_001130141.1. [P57721-2]
DR RefSeq; NP_001335167.1; NM_001348238.1. [P57721-4]
DR RefSeq; NP_001335171.1; NM_001348242.1.
DR RefSeq; NP_001335173.1; NM_001348244.1. [P57721-5]
DR RefSeq; NP_065389.2; NM_020528.2. [P57721-1]
DR AlphaFoldDB; P57721; -.
DR SMR; P57721; -.
DR BioGRID; 119851; 134.
DR IntAct; P57721; 37.
DR MINT; P57721; -.
DR STRING; 9606.ENSP00000383168; -.
DR iPTMnet; P57721; -.
DR MetOSite; P57721; -.
DR PhosphoSitePlus; P57721; -.
DR SwissPalm; P57721; -.
DR BioMuta; PCBP3; -.
DR DMDM; 296439262; -.
DR EPD; P57721; -.
DR jPOST; P57721; -.
DR MassIVE; P57721; -.
DR MaxQB; P57721; -.
DR PaxDb; P57721; -.
DR PeptideAtlas; P57721; -.
DR PRIDE; P57721; -.
DR ProteomicsDB; 57009; -. [P57721-1]
DR ProteomicsDB; 57010; -. [P57721-2]
DR ProteomicsDB; 57011; -. [P57721-3]
DR ProteomicsDB; 57012; -. [P57721-4]
DR ProteomicsDB; 57013; -. [P57721-5]
DR Antibodypedia; 24497; 86 antibodies from 20 providers.
DR DNASU; 54039; -.
DR Ensembl; ENST00000400308.5; ENSP00000383163.1; ENSG00000183570.17. [P57721-2]
DR Ensembl; ENST00000400309.5; ENSP00000383164.1; ENSG00000183570.17. [P57721-4]
DR Ensembl; ENST00000400310.5; ENSP00000383165.1; ENSG00000183570.17. [P57721-5]
DR Ensembl; ENST00000400314.5; ENSP00000383168.1; ENSG00000183570.17. [P57721-1]
DR Ensembl; ENST00000681687.1; ENSP00000505796.1; ENSG00000183570.17. [P57721-1]
DR GeneID; 54039; -.
DR KEGG; hsa:54039; -.
DR MANE-Select; ENST00000681687.1; ENSP00000505796.1; NM_001384156.1; NP_001371085.1.
DR UCSC; uc002zhp.2; human. [P57721-1]
DR CTD; 54039; -.
DR DisGeNET; 54039; -.
DR GeneCards; PCBP3; -.
DR HGNC; HGNC:8651; PCBP3.
DR HPA; ENSG00000183570; Tissue enhanced (retina).
DR MIM; 608502; gene.
DR neXtProt; NX_P57721; -.
DR OpenTargets; ENSG00000183570; -.
DR PharmGKB; PA32990; -.
DR VEuPathDB; HostDB:ENSG00000183570; -.
DR eggNOG; KOG2190; Eukaryota.
DR GeneTree; ENSGT00940000162185; -.
DR HOGENOM; CLU_022670_0_1_1; -.
DR InParanoid; P57721; -.
DR PhylomeDB; P57721; -.
DR TreeFam; TF318292; -.
DR PathwayCommons; P57721; -.
DR SignaLink; P57721; -.
DR BioGRID-ORCS; 54039; 12 hits in 1074 CRISPR screens.
DR ChiTaRS; PCBP3; human.
DR GeneWiki; PCBP3; -.
DR GenomeRNAi; 54039; -.
DR Pharos; P57721; Tbio.
DR PRO; PR:P57721; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; P57721; protein.
DR Bgee; ENSG00000183570; Expressed in right hemisphere of cerebellum and 99 other tissues.
DR ExpressionAtlas; P57721; baseline and differential.
DR Genevisible; P57721; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:1990829; F:C-rich single-stranded DNA binding; IEA:Ensembl.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:Ensembl.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0016071; P:mRNA metabolic process; NAS:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0051252; P:regulation of RNA metabolic process; IBA:GO_Central.
DR Gene3D; 3.30.1370.10; -; 3.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR Pfam; PF00013; KH_1; 3.
DR SMART; SM00322; KH; 3.
DR SUPFAM; SSF54791; SSF54791; 3.
DR PROSITE; PS50084; KH_TYPE_1; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW Ribonucleoprotein; RNA-binding.
FT CHAIN 1..371
FT /note="Poly(rC)-binding protein 3"
FT /id="PRO_0000050092"
FT DOMAIN 45..95
FT /note="KH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 129..182
FT /note="KH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 293..357
FT /note="KH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT VAR_SEQ 201..225
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009947"
FT VAR_SEQ 240
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009948"
FT VAR_SEQ 267..286
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_010014"
SQ SEQUENCE 371 AA; 39465 MW; 72BADA99CFCBDB1F CRC64;
MGEGDAFWAP SVLPHSTLST LSHHPQPQFG RRMESKVSEG GLNVTLTIRL LMHGKEVGSI
IGKKGETVKK MREESGARIN ISEGNCPERI VTITGPTDAI FKAFAMIAYK FEEDIINSMS
NSPATSKPPV TLRLVVPASQ CGSLIGKGGS KIKEIRESTG AQVQVAGDML PNSTERAVTI
SGTPDAIIQC VKQICVVMLE SPPKGATIPY RPKPASTPVI FAGGQAYTIQ GQYAIPHPDQ
LTKLHQLAMQ QTPFPPLGQT NPAFPGEKLP LHSSEEAQNL MGQSSGLDAS PPASTHELTI
PNDLIGCIIG RQGTKINEIR QMSGAQIKIA NATEGSSERQ ITITGTPANI SLAQYLINAR
LTSEVTGMGT L
