PCBP4_HUMAN
ID PCBP4_HUMAN Reviewed; 403 AA.
AC P57723; Q96AH7;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Poly(rC)-binding protein 4;
DE AltName: Full=Alpha-CP4;
GN Name=PCBP4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=10936052; DOI=10.1006/geno.2000.6244;
RA Makeyev A.V., Liebhaber S.A.;
RT "Identification of two novel mammalian genes establishes a subfamily of KH-
RT domain RNA-binding proteins.";
RL Genomics 67:301-316(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=14612387; DOI=10.1128/mcb.23.23.8405-8415.2003;
RA Chkheidze A.N., Liebhaber S.A.;
RT "A novel set of nuclear localization signals determine distributions of the
RT alphaCP RNA-binding proteins.";
RL Mol. Cell. Biol. 23:8405-8415(2003).
CC -!- FUNCTION: Single-stranded nucleic acid binding protein that binds
CC preferentially to oligo dC. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14612387}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P57723-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P57723-2; Sequence=VSP_010016;
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DR EMBL; AF176330; AAG09241.1; -; mRNA.
DR EMBL; AK023993; BAB14761.1; -; mRNA.
DR EMBL; BC003008; AAH03008.1; -; mRNA.
DR EMBL; BC004153; AAH04153.1; -; mRNA.
DR EMBL; BC017098; AAH17098.1; -; mRNA.
DR CCDS; CCDS2839.1; -. [P57723-1]
DR CCDS; CCDS2840.2; -. [P57723-2]
DR RefSeq; NP_001167571.1; NM_001174100.1. [P57723-1]
DR RefSeq; NP_065151.2; NM_020418.3. [P57723-2]
DR RefSeq; NP_127501.1; NM_033008.2. [P57723-1]
DR RefSeq; NP_127503.1; NM_033010.2. [P57723-1]
DR RefSeq; XP_005265388.1; XM_005265331.2. [P57723-1]
DR RefSeq; XP_006713331.1; XM_006713268.2.
DR RefSeq; XP_006713332.1; XM_006713269.1. [P57723-1]
DR RefSeq; XP_006713333.1; XM_006713270.1. [P57723-2]
DR AlphaFoldDB; P57723; -.
DR SMR; P57723; -.
DR BioGRID; 121351; 43.
DR IntAct; P57723; 10.
DR STRING; 9606.ENSP00000417196; -.
DR iPTMnet; P57723; -.
DR PhosphoSitePlus; P57723; -.
DR BioMuta; PCBP4; -.
DR DMDM; 12230429; -.
DR EPD; P57723; -.
DR jPOST; P57723; -.
DR MassIVE; P57723; -.
DR MaxQB; P57723; -.
DR PaxDb; P57723; -.
DR PeptideAtlas; P57723; -.
DR PRIDE; P57723; -.
DR ProteomicsDB; 57014; -. [P57723-1]
DR ProteomicsDB; 57015; -. [P57723-2]
DR Antibodypedia; 14226; 189 antibodies from 30 providers.
DR DNASU; 57060; -.
DR Ensembl; ENST00000322099.11; ENSP00000322341.7; ENSG00000090097.22. [P57723-1]
DR Ensembl; ENST00000355852.6; ENSP00000348111.2; ENSG00000090097.22. [P57723-1]
DR Ensembl; ENST00000428823.6; ENSP00000395030.2; ENSG00000090097.22. [P57723-2]
DR Ensembl; ENST00000461554.6; ENSP00000417196.1; ENSG00000090097.22. [P57723-1]
DR Ensembl; ENST00000484633.5; ENSP00000417100.1; ENSG00000090097.22. [P57723-2]
DR GeneID; 57060; -.
DR KEGG; hsa:57060; -.
DR MANE-Select; ENST00000461554.6; ENSP00000417196.1; NM_001174100.2; NP_001167571.1.
DR UCSC; uc003dcf.3; human. [P57723-1]
DR CTD; 57060; -.
DR DisGeNET; 57060; -.
DR GeneCards; PCBP4; -.
DR HGNC; HGNC:8652; PCBP4.
DR HPA; ENSG00000090097; Tissue enhanced (retina).
DR MIM; 608503; gene.
DR neXtProt; NX_P57723; -.
DR OpenTargets; ENSG00000090097; -.
DR PharmGKB; PA32991; -.
DR VEuPathDB; HostDB:ENSG00000090097; -.
DR eggNOG; KOG2190; Eukaryota.
DR GeneTree; ENSGT00940000159711; -.
DR HOGENOM; CLU_022670_0_0_1; -.
DR InParanoid; P57723; -.
DR OMA; GKAGHRI; -.
DR OrthoDB; 954970at2759; -.
DR PhylomeDB; P57723; -.
DR TreeFam; TF318292; -.
DR PathwayCommons; P57723; -.
DR Reactome; R-HSA-6804116; TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest.
DR Reactome; R-HSA-69895; Transcriptional activation of cell cycle inhibitor p21.
DR Reactome; R-HSA-9617828; FOXO-mediated transcription of cell cycle genes.
DR SignaLink; P57723; -.
DR BioGRID-ORCS; 57060; 16 hits in 1080 CRISPR screens.
DR ChiTaRS; PCBP4; human.
DR GeneWiki; PCBP4; -.
DR GenomeRNAi; 57060; -.
DR Pharos; P57723; Tbio.
DR PRO; PR:P57723; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; P57723; protein.
DR Bgee; ENSG00000090097; Expressed in right hemisphere of cerebellum and 193 other tissues.
DR ExpressionAtlas; P57723; baseline and differential.
DR Genevisible; P57723; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:MGI.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0043488; P:regulation of mRNA stability; IDA:MGI.
DR GO; GO:0051252; P:regulation of RNA metabolic process; IBA:GO_Central.
DR Gene3D; 3.30.1370.10; -; 3.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR033088; PCBP4.
DR PANTHER; PTHR10288:SF166; PTHR10288:SF166; 1.
DR Pfam; PF00013; KH_1; 3.
DR SMART; SM00322; KH; 3.
DR SUPFAM; SSF54791; SSF54791; 3.
DR PROSITE; PS50084; KH_TYPE_1; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW Ribonucleoprotein; RNA-binding.
FT CHAIN 1..403
FT /note="Poly(rC)-binding protein 4"
FT /id="PRO_0000050094"
FT DOMAIN 17..67
FT /note="KH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 101..154
FT /note="KH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 241..293
FT /note="KH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT VAR_SEQ 130..172
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010016"
FT VARIANT 198
FT /note="G -> S (in dbSNP:rs323872)"
FT /id="VAR_049681"
SQ SEQUENCE 403 AA; 41482 MW; 3D99F762A9471265 CRC64;
MSGSDGGLEE EPELSITLTL RMLMHGKEVG SIIGKKGETV KRIREQSSAR ITISEGSCPE
RITTITGSTA AVFHAVSMIA FKLDEDLCAA PANGGNVSRP PVTLRLVIPA SQCGSLIGKA
GTKIKEIRET TGAQVQVAGD LLPNSTERAV TVSGVPDAII LCVRQICAVI LESPPKGATI
PYHPSLSLGT VLLSANQGFS VQGQYGAVTP AEVTKLQQLS SHAVPFATPS VVPGLDPGTQ
TSSQEFLVPN DLIGCVIGRQ GSKISEIRQM SGAHIKIGNQ AEGAGERHVT ITGSPVSIAL
AQYLITACLE TAKSTSGGTP SSAPADLPAP FSPPLTALPT APPGLLGTPY AISLSNFIGL
KPMPFLALPP ASPGPPPGLA AYTAKMAAAN GSKKAERQKF SPY
