PCBP4_MOUSE
ID PCBP4_MOUSE Reviewed; 403 AA.
AC P57724; Q91VR3;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 1.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=Poly(rC)-binding protein 4;
DE AltName: Full=Alpha-CP4;
GN Name=Pcbp4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=10936052; DOI=10.1006/geno.2000.6244;
RA Makeyev A.V., Liebhaber S.A.;
RT "Identification of two novel mammalian genes establishes a subfamily of KH-
RT domain RNA-binding proteins.";
RL Genomics 67:301-316(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Single-stranded nucleic acid binding protein that binds
CC preferentially to oligo dC. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed, with highest levels in testis and
CC lowest in heart. {ECO:0000269|PubMed:10936052}.
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DR EMBL; AF176328; AAG09239.1; -; mRNA.
DR EMBL; BC010694; AAH10694.1; -; mRNA.
DR CCDS; CCDS23479.1; -.
DR RefSeq; NP_067542.3; NM_021567.5.
DR RefSeq; XP_006511842.1; XM_006511779.3.
DR RefSeq; XP_006511843.1; XM_006511780.1.
DR AlphaFoldDB; P57724; -.
DR SMR; P57724; -.
DR BioGRID; 208527; 2.
DR STRING; 10090.ENSMUSP00000024260; -.
DR iPTMnet; P57724; -.
DR PhosphoSitePlus; P57724; -.
DR MaxQB; P57724; -.
DR PaxDb; P57724; -.
DR PeptideAtlas; P57724; -.
DR PRIDE; P57724; -.
DR ProteomicsDB; 287799; -.
DR DNASU; 59092; -.
DR GeneID; 59092; -.
DR KEGG; mmu:59092; -.
DR UCSC; uc009rjt.3; mouse.
DR CTD; 57060; -.
DR MGI; MGI:1890471; Pcbp4.
DR eggNOG; KOG2190; Eukaryota.
DR InParanoid; P57724; -.
DR OrthoDB; 954970at2759; -.
DR PhylomeDB; P57724; -.
DR TreeFam; TF318292; -.
DR BioGRID-ORCS; 59092; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Pcbp4; mouse.
DR PRO; PR:P57724; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P57724; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; ISO:MGI.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:1902163; P:negative regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; IMP:MGI.
DR GO; GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; IMP:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0043488; P:regulation of mRNA stability; ISO:MGI.
DR GO; GO:0051252; P:regulation of RNA metabolic process; IBA:GO_Central.
DR Gene3D; 3.30.1370.10; -; 3.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR033088; PCBP4.
DR PANTHER; PTHR10288:SF166; PTHR10288:SF166; 1.
DR Pfam; PF00013; KH_1; 3.
DR SMART; SM00322; KH; 3.
DR SUPFAM; SSF54791; SSF54791; 3.
DR PROSITE; PS50084; KH_TYPE_1; 3.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome; Repeat; Ribonucleoprotein;
KW RNA-binding.
FT CHAIN 1..403
FT /note="Poly(rC)-binding protein 4"
FT /id="PRO_0000050095"
FT DOMAIN 17..67
FT /note="KH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 101..154
FT /note="KH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 241..293
FT /note="KH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT CONFLICT 235
FT /note="M -> L (in Ref. 2; AAH10694)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 41399 MW; D4DBFF490CEFE90A CRC64;
MSSSDAGLEE GPELSITLTL RMLMHGKEVG SIIGKKGETV KRIREQSSAR ITISEGSCPE
RITTITGSTA AVFHAVSMIA FKLDEDLCAA PANGGSVSRP PVTLRLVIPA SQCGSLIGKA
GTKIKEIRET TGAQVQVAGD LLPNSTERAV TVSGVPDAII LCVRQICAVI LESPPKGATI
PYHPSLSLGT VLLSANQGFS VQGQYGAVTP AEVTKLQQLS GHAVPFASPS VVPGMDPSTQ
TSSQEFLVPN DLIGCVIGRQ GSKISEIRQM SGAHIKIGNQ AEGAGERHVT ITGSPVSIAL
AQYLITACLE TAKSTSGGTP GSAPADLPTP FSPPLTALPT APPGLLGTPY AISLSNFIGL
KPVPFLALPP ASPGPPPGLA AYTAKMAAAN GSKKAERQKF SPY
