PCC1_YEAST
ID PCC1_YEAST Reviewed; 88 AA.
AC Q3E833; D6VXF6;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=EKC/KEOPS complex subunit PCC1;
DE AltName: Full=Polarized growth chromatin-associated controller 1;
GN Name=PCC1; OrderedLocusNames=YKR095W-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=12844361; DOI=10.1186/gb-2003-4-7-r45;
RA Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A.,
RA Gates K., Gaffney T.D., Philippsen P.;
RT "Reinvestigation of the Saccharomyces cerevisiae genome annotation by
RT comparison to the genome of a related fungus: Ashbya gossypii.";
RL Genome Biol. 4:R45.1-R45.13(2003).
RN [4]
RP GENOME REANNOTATION.
RX PubMed=12775844; DOI=10.1126/science.1084337;
RA Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J.,
RA Waterston R., Cohen B.A., Johnston M.;
RT "Finding functional features in Saccharomyces genomes by phylogenetic
RT footprinting.";
RL Science 301:71-76(2003).
RN [5]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE
RP EKC/KEOPS COMPLEX.
RX PubMed=16874308; DOI=10.1038/sj.emboj.7601235;
RA Kisseleva-Romanova E., Lopreiato R., Baudin-Baillieu A., Rousselle J.-C.,
RA Ilan L., Hofmann K., Namane A., Mann C., Libri D.;
RT "Yeast homolog of a cancer-testis antigen defines a new transcription
RT complex.";
RL EMBO J. 25:3576-3585(2006).
RN [6]
RP FUNCTION IN T(6)A37 FORMATION.
RX PubMed=21183954; DOI=10.1038/emboj.2010.343;
RA Srinivasan M., Mehta P., Yu Y., Prugar E., Koonin E.V., Karzai A.W.,
RA Sternglanz R.;
RT "The highly conserved KEOPS/EKC complex is essential for a universal tRNA
RT modification, t6A.";
RL EMBO J. 30:873-881(2011).
RN [7]
RP FUNCTION IN T(6)A37 FORMATION.
RX PubMed=21459853; DOI=10.1093/nar/gkr178;
RA Daugeron M.C., Lenstra T.L., Frizzarin M., El Yacoubi B., Liu X.,
RA Baudin-Baillieu A., Lijnzaad P., Decourty L., Saveanu C., Jacquier A.,
RA Holstege F.C., de Crecy-Lagard V., van Tilbeurgh H., Libri D.;
RT "Gcn4 misregulation reveals a direct role for the evolutionary conserved
RT EKC/KEOPS in the t6A modification of tRNAs.";
RL Nucleic Acids Res. 39:6148-6160(2011).
RN [8]
RP FUNCTION IN T(6)A TRNA MODIFICATION.
RX PubMed=23258706; DOI=10.1093/nar/gks1287;
RA Perrochia L., Crozat E., Hecker A., Zhang W., Bareille J., Collinet B.,
RA van Tilbeurgh H., Forterre P., Basta T.;
RT "In vitro biosynthesis of a universal t6A tRNA modification in Archaea and
RT Eukarya.";
RL Nucleic Acids Res. 41:1953-1964(2013).
RN [9]
RP FUNCTION IN THE EKC/KEOPS COMPLEX.
RX PubMed=23620299; DOI=10.1093/nar/gkt322;
RA Wan L.C., Mao D.Y., Neculai D., Strecker J., Chiovitti D., Kurinov I.,
RA Poda G., Thevakumaran N., Yuan F., Szilard R.K., Lissina E., Nislow C.,
RA Caudy A.A., Durocher D., Sicheri F.;
RT "Reconstitution and characterization of eukaryotic N6-
RT threonylcarbamoylation of tRNA using a minimal enzyme system.";
RL Nucleic Acids Res. 41:6332-6346(2013).
CC -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC formation of a threonylcarbamoyl group on adenosine at position 37
CC (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC is probably involved in the transfer of the threonylcarbamoyl moiety of
CC threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. PCC1 functions
CC as a dimerization module for the complex. The EKC/KEOPS complex also
CC promotes both telomere uncapping and telomere elongation. The complex
CC is required for efficient recruitment of transcriptional coactivators.
CC {ECO:0000269|PubMed:16874308, ECO:0000269|PubMed:21183954,
CC ECO:0000269|PubMed:21459853, ECO:0000269|PubMed:23258706,
CC ECO:0000269|PubMed:23620299}.
CC -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least BUD32,
CC CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.
CC {ECO:0000269|PubMed:16874308}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Chromosome, telomere
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CTAG/PCC1 family. {ECO:0000305}.
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DR EMBL; Z28320; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z28321; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006944; DAA09246.1; -; Genomic_DNA.
DR RefSeq; NP_878113.4; NM_001184522.3.
DR PDB; 4WX8; X-ray; 2.99 A; A/B/C=1-88.
DR PDB; 4WXA; X-ray; 2.44 A; A/B/C=1-88.
DR PDBsum; 4WX8; -.
DR PDBsum; 4WXA; -.
DR AlphaFoldDB; Q3E833; -.
DR SMR; Q3E833; -.
DR BioGRID; 37084; 18.
DR ComplexPortal; CPX-995; KEOPS complex.
DR IntAct; Q3E833; 4.
DR MINT; Q3E833; -.
DR STRING; 4932.YKR095W-A; -.
DR iPTMnet; Q3E833; -.
DR MaxQB; Q3E833; -.
DR PaxDb; Q3E833; -.
DR PRIDE; Q3E833; -.
DR EnsemblFungi; YKR095W-A_mRNA; YKR095W-A; YKR095W-A.
DR GeneID; 1500489; -.
DR KEGG; sce:YKR095W-A; -.
DR SGD; S000028512; PCC1.
DR VEuPathDB; FungiDB:YKR095W-A; -.
DR eggNOG; ENOG502S7CS; Eukaryota.
DR HOGENOM; CLU_113770_5_0_1; -.
DR InParanoid; Q3E833; -.
DR OMA; KTIIECM; -.
DR BioCyc; YEAST:G3O-32097-MON; -.
DR PRO; PR:Q3E833; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; Q3E833; protein.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0000408; C:EKC/KEOPS complex; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0031490; F:chromatin DNA binding; IDA:SGD.
DR GO; GO:1990145; P:maintenance of translational fidelity; IC:ComplexPortal.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0000723; P:telomere maintenance; IC:ComplexPortal.
DR GO; GO:0000722; P:telomere maintenance via recombination; IGI:SGD.
DR GO; GO:0070525; P:tRNA threonylcarbamoyladenosine metabolic process; IMP:SGD.
DR GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IC:ComplexPortal.
DR InterPro; IPR015419; CTAG/Pcc1.
DR PANTHER; PTHR31283; PTHR31283; 1.
DR Pfam; PF09341; Pcc1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Chromosome; Nucleus; Reference proteome; Telomere;
KW Transcription; Transcription regulation; tRNA processing.
FT CHAIN 1..88
FT /note="EKC/KEOPS complex subunit PCC1"
FT /id="PRO_0000245368"
FT STRAND 11..18
FT /evidence="ECO:0007829|PDB:4WXA"
FT HELIX 22..31
FT /evidence="ECO:0007829|PDB:4WXA"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:4WXA"
FT STRAND 43..50
FT /evidence="ECO:0007829|PDB:4WXA"
FT STRAND 53..62
FT /evidence="ECO:0007829|PDB:4WXA"
FT HELIX 63..88
FT /evidence="ECO:0007829|PDB:4WXA"
SQ SEQUENCE 88 AA; 9993 MW; B514D69352F93F7B CRC64;
MTSKREKSLD HTLELKIPFE TERQATIATK VLSPDPILKP QDFQVDYSSE KNVMLVQFRS
IDDRVLRVGV SSIIDSIKTI VEAMDVLS
