ID   PCDP1_CHLRE             Reviewed;         645 AA.
AC   A8J6X7;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Cilia- and flagella-associated protein 221 homolog {ECO:0000305};
DE   AltName: Full=Flagella-associated protein 221 {ECO:0000303|PubMed:20421426};
DE   AltName: Full=Primary ciliary dyskinesia protein 1 {ECO:0000303|PubMed:20421426};
GN   Name=CFAP221 {ECO:0000305};
GN   Synonyms=FAP221 {ECO:0000303|PubMed:20421426},
GN   PCDP1 {ECO:0000303|PubMed:20421426}; ORFNames=CHLREDRAFT_176588;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=17932292; DOI=10.1126/science.1143609;
RA   Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA   Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA   Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA   Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA   Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA   Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA   Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA   Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA   Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA   Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA   Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA   Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA   Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA   Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA   Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA   Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA   Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA   Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA   Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA   Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA   Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT   "The Chlamydomonas genome reveals the evolution of key animal and plant
RT   functions.";
RL   Science 318:245-250(2007).
RN   [2]
RP   CALMODULIN-BINDING, SUBUNIT, REGION, AND SUBCELLULAR LOCATION.
RX   PubMed=20421426; DOI=10.1083/jcb.200912009;
RA   DiPetrillo C.G., Smith E.F.;
RT   "Pcdp1 is a central apparatus protein that binds Ca2+-calmodulin and
RT   regulates ciliary motility.";
RL   J. Cell Biol. 189:601-612(2010).
CC   -!- FUNCTION: May play a role in cilium morphogenesis.
CC       {ECO:0000250|UniProtKB:A9Q751}.
CC   -!- SUBUNIT: Interacts with calmodulin; calcium-dependent. Part of the
CC       PDCP1 complex composed of CFAP46, CFAP54, CFAP74 and CFAP221; the PDCP1
CC       complex binds calmodulin. {ECO:0000269|PubMed:20421426}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000269|PubMed:20421426}. Cytoplasm
CC       {ECO:0000250|UniProtKB:A9Q751}.
CC   -!- SIMILARITY: Belongs to the PCDP1 family. {ECO:0000305}.
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DR   EMBL; DS496140; EDP00231.1; -; Genomic_DNA.
DR   RefSeq; XP_001697291.1; XM_001697239.1.
DR   AlphaFoldDB; A8J6X7; -.
DR   STRING; 3055.EDP00231; -.
DR   PaxDb; A8J6X7; -.
DR   PRIDE; A8J6X7; -.
DR   GeneID; 5722860; -.
DR   eggNOG; ENOG502QT0T; Eukaryota.
DR   HOGENOM; CLU_424783_0_0_1; -.
DR   InParanoid; A8J6X7; -.
DR   OrthoDB; 1411975at2759; -.
DR   GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR   GO; GO:0005516; F:calmodulin binding; IDA:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0003341; P:cilium movement; IEA:InterPro.
DR   Gene3D; 2.60.40.10; -; 2.
DR   InterPro; IPR029676; Gm101.
DR   InterPro; IPR013783; Ig-like_fold.
DR   PANTHER; PTHR46500; PTHR46500; 2.
PE   1: Evidence at protein level;
KW   Calmodulin-binding; Cell projection; Cilium; Cilium biogenesis/degradation;
KW   Cytoplasm; Cytoskeleton.
FT   CHAIN           1..645
FT                   /note="Cilia- and flagella-associated protein 221 homolog"
FT                   /id="PRO_0000395001"
FT   REGION          381..408
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          428..435
FT                   /note="Interaction with calmodulin"
FT                   /evidence="ECO:0000269|PubMed:20421426"
SQ   SEQUENCE   645 AA;  71053 MW;  3C668D66C212509E CRC64;
     MALASNPRRR LHEVRVQKYV VIAVTWRGAQ PMSQRARPSR VVRAAAWGMF ARCHPGAAAG
     WHRCTDLVTR SSARPAYTVA SQSFSQGLVE DAPLQNPNTL TYNRVYSKVG NTRILQAEPA
     VLNFGGYELG KVYSQVLRIR NVRASGTRFH IIPPSTPFFK ATCPAKKGLL APGMTEEVAV
     EFCPTQYRYY YDCVRVHCEE ENLLIPLHAY PVANEALFPT RVDFGRVALG QEVVRSHTLE
     CKVPVDFEYE IVEVKPNPAF RVEPARGVVP GRGRVTVDMW FCPLALTTEE AVIETEVAGV
     YVPDTMLLSA AEVGLRTKDV RGAIEARKAA AAEQQAALEK GALFRLQLAL AEEAARKVAL
     GTAPHSGQQL LTAEQPELEA GGAVHQPSAP VGSSSSGGGG GSDPAFKPEH KRTRVLDKFM
     RAVWRVVTHQ RLQRRLERIK EVLAHLGYDK QRLAEEAANP VLLVSESDRP GTAPTKYLRP
     EMVRVRPLPL YRDVLFQVHH ATDLSHYTDF DELAPFTSKV RERLVPSGLV PPLSDYPTMP
     DACKNMPYIT LEIGNRYGDD RVYGAPDPSY SPYGSLDVDY AVQPRQYDVY DSARHEAVAS
     GGVRSLRGGP GLSDSWLVRQ ICPAVPTDEQ LAKCGGTGIV NTIPN