ID   PCEB_DESHY              Reviewed;         105 AA.
AC   Q8L171;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Probable tetrachloroethene reductive dehalogenase membrane anchor protein {ECO:0000305};
GN   Name=pceB {ECO:0000303|PubMed:12057934};
GN   OrderedLocusNames=DSY2838 {ECO:0000312|EMBL:BAE84627.1};
OS   Desulfitobacterium hafniense (strain Y51).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=138119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=Y51;
RX   PubMed=12057934; DOI=10.1128/jb.184.13.3419-3428.2002;
RA   Suyama A., Yamashita M., Yoshino S., Furukawa K.;
RT   "Molecular characterization of the PceA reductive dehalogenase of
RT   desulfitobacterium sp. strain Y51.";
RL   J. Bacteriol. 184:3419-3425(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND OPERON STRUCTURE.
RC   STRAIN=Y51;
RX   PubMed=16133337; DOI=10.1007/s00253-005-0112-9;
RA   Futagami T., Tsuboi Y., Suyama A., Goto M., Furukawa K.;
RT   "Emergence of two types of nondechlorinating variants in the
RT   tetrachloroethene-halorespiring Desulfitobacterium sp. strain Y51.";
RL   Appl. Microbiol. Biotechnol. 70:720-728(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y51;
RX   PubMed=16513756; DOI=10.1128/jb.188.6.2262-2274.2006;
RA   Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA   Inatomi K., Furukawa K., Inui M., Yukawa H.;
RT   "Complete genome sequence of the dehalorespiring bacterium
RT   Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides
RT   ethenogenes 195.";
RL   J. Bacteriol. 188:2262-2274(2006).
RN   [4]
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Y51;
RX   PubMed=16957221; DOI=10.1128/aem.00979-06;
RA   Futagami T., Yamaguchi T., Nakayama S., Goto M., Furukawa K.;
RT   "Effects of chloromethanes on growth of and deletion of the pce gene
RT   cluster in dehalorespiring Desulfitobacterium hafniense strain Y51.";
RL   Appl. Environ. Microbiol. 72:5998-6003(2006).
CC   -!- FUNCTION: May act as a membrane anchor for the tetrachloroethene
CC       reductive dehalogenase PceA. {ECO:0000305|PubMed:12057934}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Located in the pceABCT gene cluster that is flanked by
CC       insertion sequences including transposase genes (PubMed:16133337).
CC       PceA, pceB and pceC are cotranscribed (PubMed:16957221).
CC       {ECO:0000269|PubMed:16133337, ECO:0000269|PubMed:16957221}.
CC   -!- DISRUPTION PHENOTYPE: Chloroform inhibits the growth of the wild-type
CC       strain, but not the growth of the PCE-nondechlorinating small deletion
CC       (SD) and large deletion (LD) variants, where the former fails to
CC       transcribe the pceABC genes caused by a deletion of the promoter and
CC       the latter lost the entire pceABCT gene cluster.
CC       {ECO:0000269|PubMed:16957221}.
CC   -!- SIMILARITY: Belongs to the PceB family. {ECO:0000305}.
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DR   EMBL; AB070709; BAC00916.1; -; Genomic_DNA.
DR   EMBL; AY706985; AAW80324.1; -; Genomic_DNA.
DR   EMBL; AP008230; BAE84627.1; -; Genomic_DNA.
DR   RefSeq; WP_011460640.1; NC_007907.1.
DR   STRING; 138119.DSY2838; -.
DR   EnsemblBacteria; BAE84627; BAE84627; DSY2838.
DR   KEGG; dsy:DSY2838; -.
DR   HOGENOM; CLU_2232152_0_0_9; -.
DR   OMA; QYGIWRY; -.
DR   OrthoDB; 1798022at2; -.
DR   Proteomes; UP000001946; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..105
FT                   /note="Probable tetrachloroethene reductive dehalogenase
FT                   membrane anchor protein"
FT                   /id="PRO_0000453977"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   105 AA;  11843 MW;  1B2D5C6FDC9FF6DE CRC64;
     MNIYDVLIWM ALGMTALLIQ YGIWRYLKGK GKDTIPLQIC GFLANFFFIF ALAWGYSSFS
     EREYQAIGMG FIFFGGTALI PAIITYRLAN HPAKKIRESS DSISA