PCFS1_ARATH
ID PCFS1_ARATH Reviewed; 416 AA.
AC Q9C710; Q8VZG9;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Polyadenylation and cleavage factor homolog 1 {ECO:0000305};
GN Name=PCFS1 {ECO:0000303|PubMed:18479511};
GN OrderedLocusNames=At1g66500 {ECO:0000312|Araport:AT1G66500};
GN ORFNames=F28G11.6 {ECO:0000312|EMBL:AAG51167.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=15236668; DOI=10.1186/1471-2164-5-39;
RA Englbrecht C.C., Schoof H., Boehm S.;
RT "Conservation, diversification and expansion of C2H2 zinc finger proteins
RT in the Arabidopsis thaliana genome.";
RL BMC Genomics 5:39-39(2004).
RN [6]
RP INTERACTION WITH CSTF77; CLPS3; CLPS5; PCFS4; PCFS5; CPSF30 AND FIPS3, GENE
RP FAMILY, AND NOMENCLATURE.
RX PubMed=18479511; DOI=10.1186/1471-2164-9-220;
RA Hunt A.G., Xu R., Addepalli B., Rao S., Forbes K.P., Meeks L.R., Xing D.,
RA Mo M., Zhao H., Bandyopadhyay A., Dampanaboina L., Marion A.,
RA Von Lanken C., Li Q.Q.;
RT "Arabidopsis mRNA polyadenylation machinery: comprehensive analysis of
RT protein-protein interactions and gene expression profiling.";
RL BMC Genomics 9:220-220(2008).
CC -!- SUBUNIT: Forms a complex with cleavage and polyadenylation specificity
CC factor (CPSF) subunits CLPS3, CLPS5, CPSF30, PCFS4, PCFS5, CSTF77 and
CC FIPS3. {ECO:0000269|PubMed:18479511}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; AC074025; AAG51167.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34516.1; -; Genomic_DNA.
DR EMBL; AY064970; AAL57625.1; -; mRNA.
DR EMBL; BT020523; AAW39024.1; -; mRNA.
DR PIR; F96690; F96690.
DR RefSeq; NP_176823.1; NM_105321.4.
DR AlphaFoldDB; Q9C710; -.
DR BioGRID; 28189; 6.
DR IntAct; Q9C710; 6.
DR STRING; 3702.AT1G66500.1; -.
DR PaxDb; Q9C710; -.
DR PRIDE; Q9C710; -.
DR ProteomicsDB; 236663; -.
DR EnsemblPlants; AT1G66500.1; AT1G66500.1; AT1G66500.
DR GeneID; 842968; -.
DR Gramene; AT1G66500.1; AT1G66500.1; AT1G66500.
DR KEGG; ath:AT1G66500; -.
DR Araport; AT1G66500; -.
DR TAIR; locus:2029002; AT1G66500.
DR eggNOG; KOG2071; Eukaryota.
DR HOGENOM; CLU_046922_0_0_1; -.
DR InParanoid; Q9C710; -.
DR OMA; ERECDLG; -.
DR OrthoDB; 321518at2759; -.
DR PhylomeDB; Q9C710; -.
DR PRO; PR:Q9C710; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C710; baseline and differential.
DR Genevisible; Q9C710; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005849; C:mRNA cleavage factor complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0000993; F:RNA polymerase II complex binding; IBA:GO_Central.
DR GO; GO:0006379; P:mRNA cleavage; IEA:InterPro.
DR GO; GO:0006378; P:mRNA polyadenylation; IBA:GO_Central.
DR GO; GO:0006369; P:termination of RNA polymerase II transcription; IBA:GO_Central.
DR InterPro; IPR045154; PCF11-like.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR15921; PTHR15921; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..416
FT /note="Polyadenylation and cleavage factor homolog 1"
FT /id="PRO_0000431349"
FT ZN_FING 254..276
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 199..220
FT /evidence="ECO:0000255"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 138
FT /note="L -> F (in Ref. 3; AAL57625)"
SQ SEQUENCE 416 AA; 46597 MW; 4E91177DED3C1240 CRC64;
MASNGSFSAQ RNANARTTMK RRSDNRGYGG GIGGYQEETN RYAPPQKRFR SQAQQQFRSG
HNPLYHHHGS NNNNVSRVSS QSYNNCGVDV IASNSSFALR NNDSNTNNYQ KPFVAGYGNP
NPQIVPLPLP YRKLDDNLSL DSLPDWVPNS RTLTPNYPVR SSNFVPNTPV FTNVQNPMNH
SNMVSVVSQS MHQPIVLSKE LTDLLSLLNN EKEKKTLEAS NSDSLPVGLS FDNPSSLNVR
HESVIKSLYS DMPRQCSSCG LRFKCQEEHS KHMDWHVRKN RSVKTTTRLG QQPKKSRGWL
ASASLWLCAA TGGETVEVAS FGGEMQKKKG KDEEPKQLMV PADEDQKNCA LCVEPFEEFF
SHEDDDWMYK DAVYLTKNGR IVHVKCMPEP RPAKDLREPS RVMSVTVPSV AKAIAC
