PCGF1_MOUSE
ID PCGF1_MOUSE Reviewed; 259 AA.
AC Q8R023;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Polycomb group RING finger protein 1;
DE AltName: Full=Nervous system Polycomb-1;
DE Short=NSPc1;
DE AltName: Full=RING finger protein 68;
GN Name=Pcgf1; Synonyms=Nspc1, Rnf68;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-259.
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=11287196; DOI=10.1016/s0925-4773(01)00288-x;
RA Nunes M., Blanc I., Maes J., Fellous M., Robert B., McElreavey K.;
RT "NSPc1, a novel mammalian Polycomb gene, is expressed in neural crest-
RT derived structures of the peripheral nervous system.";
RL Mech. Dev. 102:219-222(2001).
CC -!- FUNCTION: Component of the Polycomb group (PcG) multiprotein BCOR
CC complex, a complex required to maintain the transcriptionally
CC repressive state of some genes, such as BCL6 and the cyclin-dependent
CC kinase inhibitor, CDKN1A. Transcriptional repressor that may be
CC targeted to the DNA by BCL6; this transcription repressor activity may
CC be related to PKC signaling pathway. Represses CDKN1A expression by
CC binding to its promoter, and this repression is dependent on the
CC retinoic acid response element (RARE element). Promotes cell cycle
CC progression and enhances cell proliferation as well. May have a
CC positive role in tumor cell growth by down-regulating CDKN1A. Component
CC of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex
CC class required to maintain the transcriptionally repressive state of
CC many genes, including Hox genes, throughout development. PcG PRC1
CC complex acts via chromatin remodeling and modification of histones; it
CC mediates monoubiquitination of histone H2A 'Lys-119', rendering
CC chromatin heritably changed in its expressibility. Within the PRC1-like
CC complex, regulates RNF2 ubiquitin ligase activity. Regulates the
CC expression of DPPA4 and NANOG in the NT2 embryonic carcinoma cells.
CC {ECO:0000250|UniProtKB:Q9BSM1}.
CC -!- SUBUNIT: Interacts with BCORL1, forming heterodimers (By similarity).
CC The PCGF1-BCORL1 heterodimeric complex interacts with the KDM2B-SKP1
CC heterodimeric complex to form a homotetrameric polycomb repression
CC complex 1 (PRC1.1) (By similarity). Component of the repressive BCOR
CC complex containing a Polycomb group subcomplex at least composed of
CC RYBP, RING1 and RNF2/RING2 (By similarity). Specifically interacts with
CC BCOR, RING1 and RNF2/RING2 (By similarity). Component of a PRC1-like
CC complex (By similarity). Interacts with CBX6, CBX7 and CBX8 (By
CC similarity). Interacts with DPPA4, NANOG, POU5F1 and RYBP (By
CC similarity). {ECO:0000250|UniProtKB:Q9BSM1}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9BSM1}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the otic vesicle, urogenital bud and
CC dorsal root ganglia at 10.5 dpc, in the neural tube and neural crest
CC cell derivatives of the peripheral nervous system at 11.5 dpc.
CC {ECO:0000269|PubMed:11287196}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH28560.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC104324; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC028560; AAH28560.1; ALT_INIT; mRNA.
DR CCDS; CCDS20270.1; -.
DR RefSeq; NP_932109.1; NM_197992.1.
DR AlphaFoldDB; Q8R023; -.
DR SMR; Q8R023; -.
DR BioGRID; 213711; 32.
DR IntAct; Q8R023; 29.
DR STRING; 10090.ENSMUSP00000130614; -.
DR iPTMnet; Q8R023; -.
DR PhosphoSitePlus; Q8R023; -.
DR EPD; Q8R023; -.
DR MaxQB; Q8R023; -.
DR PaxDb; Q8R023; -.
DR PeptideAtlas; Q8R023; -.
DR PRIDE; Q8R023; -.
DR ProteomicsDB; 287965; -.
DR Antibodypedia; 1867; 166 antibodies from 24 providers.
DR DNASU; 69837; -.
DR Ensembl; ENSMUST00000165164; ENSMUSP00000130614; ENSMUSG00000069678.
DR GeneID; 69837; -.
DR KEGG; mmu:69837; -.
DR UCSC; uc009cmg.1; mouse.
DR CTD; 84759; -.
DR MGI; MGI:1917087; Pcgf1.
DR VEuPathDB; HostDB:ENSMUSG00000069678; -.
DR eggNOG; KOG2660; Eukaryota.
DR GeneTree; ENSGT00940000159651; -.
DR InParanoid; Q8R023; -.
DR OMA; ISECSHT; -.
DR OrthoDB; 1203951at2759; -.
DR PhylomeDB; Q8R023; -.
DR TreeFam; TF324206; -.
DR BioGRID-ORCS; 69837; 2 hits in 61 CRISPR screens.
DR ChiTaRS; Pcgf1; mouse.
DR PRO; PR:Q8R023; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8R023; protein.
DR Bgee; ENSMUSG00000069678; Expressed in secondary oocyte and 106 other tissues.
DR ExpressionAtlas; Q8R023; baseline and differential.
DR Genevisible; Q8R023; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0031519; C:PcG protein complex; IDA:UniProtKB.
DR GO; GO:0035102; C:PRC1 complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IBA:GO_Central.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR GO; GO:0035518; P:histone H2A monoubiquitination; ISO:MGI.
DR GO; GO:0036353; P:histone H2A-K119 monoubiquitination; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR032443; RAWUL.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF16207; RAWUL; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
FT CHAIN 2..259
FT /note="Polycomb group RING finger protein 1"
FT /id="PRO_0000277856"
FT ZN_FING 47..86
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 86..247
FT /note="Necessary for repressor activity"
FT /evidence="ECO:0000250"
FT REGION 150..255
FT /note="Required for the interaction with the KDM2B-SKP1
FT heterodimeric complex"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
FT REGION 167..255
FT /note="RING-finger and WD40-associated ubiquitin-like
FT domain (RAWUL); sufficient for interaction with BCOR and
FT BCORL1"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
FT MOD_RES 3
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
FT CROSSLNK 24
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
FT CROSSLNK 88
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BSM1"
SQ SEQUENCE 259 AA; 30318 MW; B631543B0DEA2954 CRC64;
MASPQGGQIA IAMRLRNQLQ SVYKMDPLRN EEEVRVKIKD LNEHIVCCLC AGYFVDATTI
TECLHTFCKS CIVKYLQTSK YCPMCNIKIH ETQPLLNLKL DRVMQDIVYK LVPGLQDSEE
KRIREFYQSR GLDRVSQPSG EEPALSNLGL PFSSFDHSKA HYYRYDEQLS LCLERLSSGK
DKNKNVLQNK YVRCSVRAEV RHLRRVLCHR LMLNPQHVQL LFDNEVLPDH MTMKQLWLSR
WFGKPSPLLL QYSVKEKRR
