PCH2_ORYSJ
ID PCH2_ORYSJ Reviewed; 475 AA.
AC Q7XK25; A0A0P0WBH8; Q0JCD2; U3N475;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 4.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Pachytene checkpoint protein 2 homolog {ECO:0000305};
DE AltName: Full=Protein CENTRAL REGION COMPONENT 1 {ECO:0000303|PubMed:23943860};
GN Name=CRC1 {ECO:0000303|PubMed:23943860};
GN OrderedLocusNames=Os04g0479000, LOC_Os04g40290;
GN ORFNames=OsJ_15194, OSJNBa0044K18.20;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PAIR1 AND ZEP1,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23943860; DOI=10.1105/tpc.113.113175;
RA Miao C., Tang D., Zhang H., Wang M., Li Y., Tang S., Yu H., Gu M.,
RA Cheng Z.;
RT "Central region component1, a novel synaptonemal complex component, is
RT essential for meiotic recombination initiation in rice.";
RL Plant Cell 25:2998-3009(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Plays a key role in chromosome recombination during meiosis.
CC Required for the initiation of meiotic recombination and the
CC recruitment of PAIR2 onto meiotic chromosomes. Essential for meiotic
CC DNA double-strand break (DSB) formation.
CC -!- SUBUNIT: Interacts with PAIR1 and ZEP1. {ECO:0000269|PubMed:23943860}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23943860}. Chromosome
CC {ECO:0000269|PubMed:23943860}. Note=Appears as punctuate foci on
CC chromosomes at leptotene. Colocalizes with ZEP1 to the central region
CC of the synaptonemal complex. {ECO:0000269|PubMed:23943860}.
CC -!- TISSUE SPECIFICITY: Highly expressed in young panicles.
CC {ECO:0000269|PubMed:23943860}.
CC -!- DISRUPTION PHENOTYPE: Male and female sterility due to defects in
CC meiosis. {ECO:0000269|PubMed:23943860}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. PCH2 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF15005.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAE05878.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAZ31098.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; KF245924; AGW22199.1; -; mRNA.
DR EMBL; AL606595; CAE05878.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF15005.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014960; BAS89715.1; -; Genomic_DNA.
DR EMBL; CM000141; EAZ31098.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015637286.1; XM_015781800.1.
DR AlphaFoldDB; Q7XK25; -.
DR SMR; Q7XK25; -.
DR STRING; 4530.OS04T0479000-01; -.
DR PaxDb; Q7XK25; -.
DR PRIDE; Q7XK25; -.
DR EnsemblPlants; Os04t0479000-02; Os04t0479000-02; Os04g0479000.
DR GeneID; 4336167; -.
DR Gramene; Os04t0479000-02; Os04t0479000-02; Os04g0479000.
DR KEGG; osa:4336167; -.
DR eggNOG; KOG0744; Eukaryota.
DR InParanoid; Q7XK25; -.
DR OrthoDB; 1036414at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR ExpressionAtlas; Q7XK25; baseline and differential.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:UniProtKB.
DR GO; GO:0051598; P:meiotic recombination checkpoint signaling; IBA:GO_Central.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR001270; ClpA/B.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044539; Pch2-like.
DR PANTHER; PTHR45991; PTHR45991; 1.
DR Pfam; PF00004; AAA; 1.
DR PRINTS; PR00300; CLPPROTEASEA.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chromosome; Meiosis; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..475
FT /note="Pachytene checkpoint protein 2 homolog"
FT /id="PRO_0000410928"
FT BINDING 217..224
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 475 AA; 51508 MW; F70313F660DCCDEA CRC64;
MSAPMEVSFS APPPPDAASA AAAAPSLVPA VSAAAVAATT VSCSPQPPTG SPSADDRILV
SVEVLLHATS TARAEDVCAA VERMLEARSL SYVDGPVPIP NDDPFLLANV KRIQICDTDE
WTENHKVLLF WQVRPVVHVF QLSEDGPGEE PGEDDTLSSF NEWALPAKEF DGLWESLLYE
VGLKQRLLRY AASALLFTEK GVDPCLVSWN RIVLLHGPPG TGKTSLCKAL AQKLSIRFKS
RYSMCQLIEV NAHSLFSKWF SESGKLVAKL FQKIQEMVEE ESNLVFVLID EVESLAAARQ
AAISGSEPSD SIRVVNALLT QMDKLKSWPN VIILTTSNIT TAIDIAFVDR ADIKAYVGPP
TLQARYEILR SCLQELLRVG ILTHTQGGNS LCLLSYFSLM ENQHCPEVAD PHGSVHLSGL
LHKAAEICEG LSGRTLRKLP FLAHASVANP SCCDASAFLH ALIQTAQREL SESRG
