ID   PCH2_PIG                Reviewed;         431 AA.
AC   D3K5L7;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Pachytene checkpoint protein 2 homolog;
DE   AltName: Full=Thyroid hormone receptor interactor 13;
DE   AltName: Full=Thyroid receptor-interacting protein 13;
DE            Short=TR-interacting protein 13;
DE            Short=TRIP-13;
GN   Name=TRIP13; Synonyms=PCH2;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Yg L.;
RL   Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a key role in chromosome recombination and chromosome
CC       structure development during meiosis. Required at early steps in
CC       meiotic recombination that leads to non-crossovers pathways. Also
CC       needed for efficient completion of homologous synapsis by influencing
CC       crossover distribution along the chromosomes affecting both crossovers
CC       and non-crossovers pathways. Also required for development of higher-
CC       order chromosome structures and is needed for synaptonemal-complex
CC       formation. In males, required for efficient synapsis of the sex
CC       chromosomes and for sex body formation. Promotes early steps of the DNA
CC       double-strand breaks (DSBs) repair process upstream of the assembly of
CC       RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from
CC       synapsed chromosomes. Plays a role in mitotic spindle assembly
CC       checkpoint (SAC) activation (By similarity).
CC       {ECO:0000250|UniProtKB:Q15645, ECO:0000250|UniProtKB:Q3UA06}.
CC   -!- SUBUNIT: Specifically interacts with the ligand binding domain of the
CC       thyroid receptor (TR). This interaction does not require the presence
CC       of thyroid hormone for its interaction (By similarity). Interacts with
CC       proteasome subunit PSMA8; to participate in meiosis progression during
CC       spermatogenesis (By similarity). {ECO:0000250,
CC       ECO:0000250|UniProtKB:Q3UA06}.
CC   -!- SIMILARITY: Belongs to the AAA ATPase family. PCH2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; GU373695; ADC38895.1; -; mRNA.
DR   RefSeq; NP_001182300.1; NM_001195371.1.
DR   AlphaFoldDB; D3K5L7; -.
DR   SMR; D3K5L7; -.
DR   PRIDE; D3K5L7; -.
DR   GeneID; 100337674; -.
DR   KEGG; ssc:100337674; -.
DR   CTD; 9319; -.
DR   InParanoid; D3K5L7; -.
DR   OrthoDB; 1036414at2759; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005694; C:chromosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0006302; P:double-strand break repair; ISS:UniProtKB.
DR   GO; GO:0051598; P:meiotic recombination checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0007130; P:synaptonemal complex assembly; ISS:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR003960; ATPase_AAA_CS.
DR   InterPro; IPR001270; ClpA/B.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR044539; Pch2-like.
DR   PANTHER; PTHR45991; PTHR45991; 1.
DR   Pfam; PF00004; AAA; 1.
DR   PRINTS; PR00300; CLPPROTEASEA.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00674; AAA; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; ATP-binding; Differentiation; Meiosis; Nucleotide-binding;
KW   Oogenesis; Reference proteome; Spermatogenesis.
FT   CHAIN           1..431
FT                   /note="Pachytene checkpoint protein 2 homolog"
FT                   /id="PRO_0000410922"
FT   BINDING         179..186
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q15645"
SQ   SEQUENCE   431 AA;  48462 MW;  70C2CFEFF9895955 CRC64;
     MDEAVGDLKQ ALPCVAEAPT VHVEVHQRSG STAKKEDVKL NVRKLLNRHN IVFGDYTWTE
     FDEPFLTRNV QSVSIVDTEL KVKDPQPIDL STCTVALHVF QLNEGGPSSE NLEEETENII
     AANHWLLPAA EFHGLWDSLV YDAEVKSHLL DYVMTTLLFS DKNVDSNLIT WNRVVLLHGP
     PGTGKTSLCK ALAQKLTIRL SSRYRYGQLI EINSHSLFSK WFSESGKLVT RMFQMIQDLI
     DDKDALVFVL IDEVESLTAA RNACRAGTEP SDAIRVVNAV LTQIDQIKRH SNVVILTTSN
     ITERIDVAFV DRADIRQYVG PPSAAAIFKI YLSCLEELMK CQIIYPRQQL LTLRELEMIG
     FIENNVSKLS LLLSEISRKS EGLSGRVLRK LPFLAHALYI QAPTVTIEGF LQALSLAVDR
     QFEDRKKLSC V