PCH2_RAT
ID PCH2_RAT Reviewed; 432 AA.
AC Q5XHZ9;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Pachytene checkpoint protein 2 homolog;
DE AltName: Full=Thyroid hormone receptor interactor 13;
DE AltName: Full=Thyroid receptor-interacting protein 13;
DE Short=TR-interacting protein 13;
DE Short=TRIP-13;
GN Name=Trip13; Synonyms=Pch2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Plays a key role in chromosome recombination and chromosome
CC structure development during meiosis. Required at early steps in
CC meiotic recombination that leads to non-crossovers pathways. Also
CC needed for efficient completion of homologous synapsis by influencing
CC crossover distribution along the chromosomes affecting both crossovers
CC and non-crossovers pathways. Also required for development of higher-
CC order chromosome structures and is needed for synaptonemal-complex
CC formation. In males, required for efficient synapsis of the sex
CC chromosomes and for sex body formation. Promotes early steps of the DNA
CC double-strand breaks (DSBs) repair process upstream of the assembly of
CC RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from
CC synapsed chromosomes. Plays a role in mitotic spindle assembly
CC checkpoint (SAC) activation (By similarity).
CC {ECO:0000250|UniProtKB:Q15645, ECO:0000250|UniProtKB:Q3UA06}.
CC -!- SUBUNIT: Specifically interacts with the ligand binding domain of the
CC thyroid receptor (TR). This interaction does not require the presence
CC of thyroid hormone for its interaction (By similarity). Interacts with
CC proteasome subunit PSMA8; to participate in meiosis progression during
CC spermatogenesis (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:Q3UA06}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. PCH2 subfamily.
CC {ECO:0000305}.
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DR EMBL; BC083900; AAH83900.1; -; mRNA.
DR RefSeq; NP_001011930.1; NM_001011930.1.
DR RefSeq; XP_008756903.1; XM_008758681.1.
DR AlphaFoldDB; Q5XHZ9; -.
DR SMR; Q5XHZ9; -.
DR STRING; 10116.ENSRNOP00000021532; -.
DR PhosphoSitePlus; Q5XHZ9; -.
DR jPOST; Q5XHZ9; -.
DR PaxDb; Q5XHZ9; -.
DR PRIDE; Q5XHZ9; -.
DR Ensembl; ENSRNOT00000021532; ENSRNOP00000021532; ENSRNOG00000015810.
DR GeneID; 292206; -.
DR KEGG; rno:292206; -.
DR UCSC; RGD:1308516; rat.
DR CTD; 9319; -.
DR RGD; 1308516; Trip13.
DR eggNOG; KOG0744; Eukaryota.
DR GeneTree; ENSGT00390000017432; -.
DR InParanoid; Q5XHZ9; -.
DR OMA; VQIHVEV; -.
DR OrthoDB; 1036414at2759; -.
DR PhylomeDB; Q5XHZ9; -.
DR TreeFam; TF313507; -.
DR PRO; PR:Q5XHZ9; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000015810; Expressed in thymus and 18 other tissues.
DR GO; GO:0005694; C:chromosome; IBA:GO_Central.
DR GO; GO:0001673; C:male germ cell nucleus; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0006302; P:double-strand break repair; ISS:UniProtKB.
DR GO; GO:0007144; P:female meiosis I; ISO:RGD.
DR GO; GO:0007141; P:male meiosis I; ISO:RGD.
DR GO; GO:0051598; P:meiotic recombination checkpoint signaling; IBA:GO_Central.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0001556; P:oocyte maturation; ISO:RGD.
DR GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; ISS:UniProtKB.
DR GO; GO:0007286; P:spermatid development; ISO:RGD.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR GO; GO:0007130; P:synaptonemal complex assembly; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR001270; ClpA/B.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044539; Pch2-like.
DR PANTHER; PTHR45991; PTHR45991; 1.
DR Pfam; PF00004; AAA; 1.
DR PRINTS; PR00300; CLPPROTEASEA.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00674; AAA; 1.
PE 2: Evidence at transcript level;
KW Acetylation; ATP-binding; Differentiation; Meiosis; Nucleotide-binding;
KW Oogenesis; Reference proteome; Spermatogenesis.
FT CHAIN 1..432
FT /note="Pachytene checkpoint protein 2 homolog"
FT /id="PRO_0000084784"
FT BINDING 179..186
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q15645"
SQ SEQUENCE 432 AA; 48434 MW; D15D10A47EA81BA8 CRC64;
MDDAVGDLKQ ALPCVAESPA VHVEVLQRSG STAKKEDIKQ SVYRLLKRHN IVFGDYVWTE
FDEPFLTRNV QSVSIVDTEL KAKDPQPIDL SACTIALHIF QLNEEGPSSE NLDEETENII
AASHWVLPAA EFHGLWDSLV YDVEVKSHLL DYVMTTLLFS DKNVDSNLIT WNRVVLLHGP
PGTGKTSLCK ALAQKLTIRL SSRYRYGQLI EINSHSLFSK WFSESGKLVT KMFQKIQDLI
DDKEALVFVL IDEVESLTAA RNACRAGAEP SDAIRVVNAV LTQIDQIKRH SNVVILTTSN
ITEKIDVAFV DRADIKQYIG PPSAAAIFKI YLSCLEELMK CQIIYPRQQL LTLRELEMIG
FIENNVSKLS LLLSEISRKS EGLSGRVLRK LPFLAHALYI QAPSVTIEGF LQALSLAVDK
QFEEKKKLSA HV
