PCH2_YEAST
ID PCH2_YEAST Reviewed; 564 AA.
AC P38126; D6VQI1;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Pachytene checkpoint protein 2;
GN Name=PCH2; OrderedLocusNames=YBR186W; ORFNames=YBR1308;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7871891; DOI=10.1002/yea.320101116;
RA Demolis N., Jacquet M., Mallet L.;
RT "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes
RT encoding ribosomal proteins and six putative new genes, one of which
RT encodes a putative transcriptional factor.";
RL Yeast 10:1511-1525(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=10319812; DOI=10.1016/s0092-8674(00)80741-2;
RA San-Segundo P.A., Roeder G.S.;
RT "Pch2 links chromatin silencing to meiotic checkpoint control.";
RL Cell 97:313-324(1999).
RN [5]
RP REVISION OF GENE MODEL.
RX PubMed=10734188; DOI=10.1093/nar/28.8.1700;
RA Davis C.A., Grate L., Spingola M., Ares M. Jr.;
RT "Test of intron predictions reveals novel splice sites, alternatively
RT spliced mRNAs and new introns in meiotically regulated genes of yeast.";
RL Nucleic Acids Res. 28:1700-1706(2000).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11502169; DOI=10.1021/bi010277r;
RA Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S.,
RA Schmitter J.-M.;
RT "Yeast mitochondrial dehydrogenases are associated in a supramolecular
RT complex.";
RL Biochemistry 40:9758-9769(2001).
CC -!- FUNCTION: Required for the pachytene checkpoint, the meiotic checkpoint
CC that prevents chromosome segregation when defects in recombination and
CC synaptonemal complex formation occurred. Represses meiotic
CC recombination in the rDNA, probably by excluding the meiosis-specific
CC protein HOP1 from the nucleolar region. {ECO:0000269|PubMed:10319812}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:10319812}.
CC Chromosome {ECO:0000269|PubMed:10319812}. Note=The majority of the
CC protein localizes to the nucleolar region containing the ribosomal RNA
CC genes (rDNA), a tandem array of 100-150 repeats on chromosome XII.
CC Localization is SIR2-dependent.
CC -!- INDUCTION: Induced at the onset of meiosis I and reaches a maximum
CC level during pachytene (at protein level).
CC {ECO:0000269|PubMed:10319812}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. PCH2 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB60280.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA85147.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U02073; AAB60280.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z36055; CAA85147.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006936; DAA07301.1; -; Genomic_DNA.
DR PIR; S46058; S46058.
DR RefSeq; NP_009745.2; NM_001178534.1.
DR AlphaFoldDB; P38126; -.
DR SMR; P38126; -.
DR BioGRID; 32884; 70.
DR DIP; DIP-4661N; -.
DR IntAct; P38126; 4.
DR MINT; P38126; -.
DR STRING; 4932.YBR186W; -.
DR iPTMnet; P38126; -.
DR PaxDb; P38126; -.
DR PRIDE; P38126; -.
DR EnsemblFungi; YBR186W_mRNA; YBR186W; YBR186W.
DR GeneID; 852484; -.
DR KEGG; sce:YBR186W; -.
DR SGD; S000000390; PCH2.
DR VEuPathDB; FungiDB:YBR186W; -.
DR eggNOG; KOG0744; Eukaryota.
DR GeneTree; ENSGT00940000170021; -.
DR HOGENOM; CLU_028208_3_0_1; -.
DR InParanoid; P38126; -.
DR OMA; VCIEEMI; -.
DR BioCyc; YEAST:G3O-29129-MON; -.
DR PRO; PR:P38126; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38126; protein.
DR GO; GO:0005694; C:chromosome; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IDA:SGD.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:SGD.
DR GO; GO:0051598; P:meiotic recombination checkpoint signaling; IMP:SGD.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044539; Pch2-like.
DR PANTHER; PTHR45991; PTHR45991; 1.
DR Pfam; PF00004; AAA; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 1: Evidence at protein level;
KW ATP-binding; Chromosome; Meiosis; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..564
FT /note="Pachytene checkpoint protein 2"
FT /id="PRO_0000084789"
FT BINDING 314..321
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 564 AA; 63460 MW; 9B40C41368672877 CRC64;
MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL LKAVVKRKLE
SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR SLAKVLLHQF SSINGKVNTV
NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL NFHEKINLGQ HIDSILDSEE TNESDTYHMG
SVDEFIIYPF CCLEEQDELK NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP
LSKQTLNLVN ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI
TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG IIIELSCARI
FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV EAIASSRTNL SSRNESTDGI
RVVNTLLTQL DRLKKYHNFL ALATSNLLDS LDDAFVDRAD GVFYVGNPTA EGILHILKVC
IEEMITSGII LFHARSTGVK FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT
FPVDDDEFVL ALAMSARKLS AARK
