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PCHF_PSEAB
ID   PCHF_PSEAB              Reviewed;        1809 AA.
AC   A0A0H2ZGJ4;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Pyochelin synthase PchF {ECO:0000305};
DE            EC=6.2.1.69 {ECO:0000269|PubMed:10555976};
DE   AltName: Full=L-cysteine--[L-cysteinyl-carrier protein] ligase {ECO:0000305};
DE   AltName: Full=Nonribosomal peptide synthase PchF {ECO:0000305};
GN   Name=pchF {ECO:0000303|PubMed:10555976};
GN   OrderedLocusNames=PA14_09280 {ECO:0000312|EMBL:ABJ13498.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES,
RP   PATHWAY, DOMAIN, AND MUTAGENESIS OF 1606-CYS-SER-1607.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=10555976; DOI=10.1021/bi991787c;
RA   Quadri L.E., Keating T.A., Patel H.M., Walsh C.T.;
RT   "Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore
RT   pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase
RT   activity from PchD, PchE, and PchF.";
RL   Biochemistry 38:14941-14954(1999).
CC   -!- FUNCTION: Involved in the biosynthesis of the siderophore pyochelin
CC       (PubMed:10555976). Adenylates L-cysteine and loads it onto its peptidyl
CC       carrier domain via a thioester linkage to the phosphopanthetheine
CC       moiety (PubMed:10555976). Then forms a peptide bond between the
CC       salicyl-thiazolinyl intermediate bound to the second carrier domain of
CC       PchE and the cysteine bound to its own peptidyl carrier domain to form
CC       the salicyl-thiazolinyl-cysteinyl-S-PCP2 intermediate. It subsequently
CC       cyclizes the C-terminal cysteine to form the second thiazoline
CC       heterocycle in the salicyl-thiazolinyl-thiazolinyl-S-PCP2 intermediate
CC       (PubMed:10555976). When this intermediate is released by the action of
CC       a thioesterase, it produces the tricyclic acid hydroxyphenyl-thiazolyl-
CC       thiazolinyl-carboxylic acid (HPTT-COOH), an advanced intermediate
CC       containing the aryl-4,2-bis-heterocyclic skeleton of the bithiazoline
CC       class of siderophores (PubMed:10555976). {ECO:0000269|PubMed:10555976}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + holo-[peptidyl-carrier protein] + L-cysteine = AMP +
CC         diphosphate + L-cysteinyl-[peptidyl-carrier protein];
CC         Xref=Rhea:RHEA:61680, Rhea:RHEA-COMP:11480, Rhea:RHEA-COMP:15906,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:64479, ChEBI:CHEBI:144926, ChEBI:CHEBI:456215;
CC         EC=6.2.1.69; Evidence={ECO:0000269|PubMed:10555976};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61681;
CC         Evidence={ECO:0000269|PubMed:10555976};
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000269|PubMed:10555976};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=537 uM for L-cysteine {ECO:0000269|PubMed:10555976};
CC         Note=kcat is 415 min(-1) with L-cysteine as substrate.
CC         {ECO:0000269|PubMed:10555976};
CC   -!- PATHWAY: Siderophore biosynthesis. {ECO:0000269|PubMed:10555976}.
CC   -!- DOMAIN: Modular protein that contains a condensation/cyclization domain
CC       involved in the cyclization of the cysteine, an adenylation domain
CC       which activates the cysteine residue into an aminoacyl-AMP ester, a
CC       peptidyl carrier protein (PCP2) domain which bears a
CC       phosphopantetheinyl arm to attach the activated cysteine and a
CC       thioesterase domain (TE) that may release the newly synthesized peptide
CC       from the enzyme. {ECO:0000305|PubMed:10555976}.
CC   -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
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DR   EMBL; CP000438; ABJ13498.1; -; Genomic_DNA.
DR   RefSeq; WP_011666540.1; NC_008463.1.
DR   EnsemblBacteria; ABJ13498; ABJ13498; PA14_09280.
DR   KEGG; pau:PA14_09280; -.
DR   HOGENOM; CLU_000022_2_15_6; -.
DR   OMA; GVACHGY; -.
DR   BioCyc; PAER208963:G1G74-774-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR   GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR   GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR   Gene3D; 1.10.10.1830; -; 1.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   Gene3D; 3.30.300.30; -; 2.
DR   Gene3D; 3.30.559.10; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR010071; AA_adenyl_domain.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020459; AMP-binding.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   InterPro; IPR013217; Methyltransf_12.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR041464; TubC_N.
DR   InterPro; IPR044894; TubC_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF00668; Condensation; 1.
DR   Pfam; PF08242; Methyltransf_12; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   Pfam; PF18563; TubC_N; 1.
DR   PRINTS; PR00154; AMPBINDING.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR01733; AA-adenyl-dom; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
DR   PROSITE; PS50075; CARRIER; 1.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE   1: Evidence at protein level;
KW   Ligase; Phosphopantetheine; Phosphoprotein.
FT   CHAIN           1..1809
FT                   /note="Pyochelin synthase PchF"
FT                   /id="PRO_0000454827"
FT   DOMAIN          1407..1488
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   REGION          69..490
FT                   /note="Condensation/cyclization"
FT                   /evidence="ECO:0000305"
FT   REGION          520..915
FT                   /note="Adenylation"
FT                   /evidence="ECO:0000305"
FT   REGION          1584..1797
FT                   /note="Thioesterase"
FT                   /evidence="ECO:0000305"
FT   MOD_RES         1442
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   MUTAGEN         1606..1607
FT                   /note="CS->AA: Fails to release HPTT-COOH."
FT                   /evidence="ECO:0000269|PubMed:10555976"
SQ   SEQUENCE   1809 AA;  197109 MW;  9FB6F39B1726D6E4 CRC64;
     MSLGELLETC RSRRIELWSE AGRLRYRAPQ GALDAGLAER LRAEREALLE HLEGGPGWRA
     EPDLAHQRFP LTPVQAAYVL GRQAAFDYGG NACQLYAEYD WPADTDPARL EAAWNAMVER
     HPMLRAVIED NAWQRVLPEV PWQRLTVHAC AGLDEAAFQA HLERVRERLD HACAALDQWP
     VLRPELSIGR DDCVLHCSVD FTLVDYASLQ LLLGEWRRRY LDPQWTAEPL EATFRDYVGV
     EQRRRQSPAW QRDRDWWLAR LDALPGRPDL PLRAQPDTRS TRFRHFHARL DEAAWQALGA
     RAGEHGLSAA GVALAAFAET IGRWSQAPAF CLNLTVLNRP PLHPQLAQVL GDFTALSLLA
     VDSRHGDSFV ERARRIGEQM FDDLDHPTFS GVDLLRELAR RRGRGADLMP VVFTSGIGSV
     QRLLGDGEAP RAPRYMISQT PQVWLDCQVT DQFGGLEIGW DVRLGLFPEG QAEAMFDDFV
     GLLRRLAQSP RAWTDGDATE PVEAPPQALP GSARSIAAGF AERALLTPDA TVIHDAAGSY
     SYRQVAQHAS ALRRVLEAHG AGRGRRVAVM LPKSAAQLVA VIGILQAGAA YVPVDIRQPP
     LRRQAILASP EVVAWVCLES DVPNAGCACV AIDRLAADSA WPPPPAAEVA ADDLAYVIYT
     SGSTGTPKGV MLSHAAVSNT LLDINQRYGV DANDRVLGLA ELSFDLSVYD FFGATAAGAQ
     VVLPDPARGS DPSHWAELLE RHAITLWNSV PAQGQMLIDY LESEPQRHLP GPRCVLWSGD
     WIPVSLPTRW WRRWPDSALF SLGGATEAAI WSIEQPIRPQ HTELASIPYG RALRGQSVEV
     LDARGRRCPP GVRGEIHIGG VGLALGYAGD PQRTAERFVR HPDGRRLYRT GDLGRYLADG
     SIEFLGREDD QVKIRGHRIE LAELDAALCA HPQVNLAATV VLGETHERSL ASFVTLHAPA
     EAGEDPRTAL DTVRQRAAQA LRRDWGSEEG IAAAVAALDR ACLASLAAWL AGSGLFASAT
     PLDFATLCQR LGIAEARQRL LRHWLRQLEE GGYLRAEGEG WLGCAERPAQ SPEDAWTAFA
     GCAPAALWPA ELVAYLRDSA QSLGEQLAGR ISPAALMFPQ GSARIAEAMY SQGLHAQALH
     EAMAEAIAAI VERQPQRRWR LLELGAGTAA ASRAVIARLA PLVQRGTEVD YLFTDVSSYF
     LAAARERFAD QPWVRFGRFD MNGDLLDQGV APHSVDILLS SGALNNALDT PALLAGLREL
     LSADAWLVIQ ELTREHNEIS VSQSLMMENP RDLRDERRQL FVHTGQWLEW LAAQGGDLAC
     GVVPPGSALD LLGYDVLLAR CKTDRARLEP AELLAFVEAR VPRYMLPAQL RVLERLPVTG
     NGKIDRKALT GFARQPQADL RHGVAQAPAD ELESALLALW REVLDNPSLG VEQDFFGAGG
     DSLLIAQLIA RLRERLESAR RHPFDRLLRW ALSQPTPRGL AERLRSAPEE GRGPALAAAR
     GVAPAQTGMS RAPLAEGAVA LDPLVRLVPG EGVPRVLVHE GLGTLLPYRP LLRALGEGRP
     LLGLAVHDSD AYLAIPAEYL NACLGRRYAE ALHGAGLREV DLLGYCSGGL VALETAKSLL
     QRGVRVRQLD IVSSYRIPYR VDDERLLLFS FAATLGLDTA ALGFPAPERL GQAVQAALAQ
     TPERLGAEAL AGLPGLADLV ALRGRVLQAA SGSADAASVE RDTLYRLFCH SVRASQAEAL
     EPYVGALRLF VPDAGNPLVP RYAEALETQW RAAALGACGI HEVPGGHFDC LGEALAQSLS
     KPMPEEASQ
 
 
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