ID   PCHTP_TRISP             Reviewed;         424 AA.
AC   D4N4Z9; E5SHY6; P86477;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Poly-cysteine and histidine-tailed protein;
DE            Short=Ts-PCHTP;
DE   Flags: Precursor;
GN   ORFNames=Tsp_04041;
OS   Trichinella spiralis (Trichina worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia;
OC   Trichinellida; Trichinellidae; Trichinella.
OX   NCBI_TaxID=6334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 118-133, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Larva;
RX   PubMed=20967224; DOI=10.1371/journal.pone.0013343;
RA   Radoslavov G., Jordanova R., Teofanova D., Georgieva K., Hristov P.,
RA   Salomone-Stagni M., Liebau E., Bankov I.;
RT   "A novel secretory poly-cysteine and histidine-tailed metalloprotein (Ts-
RT   PCHTP) from Trichinella spiralis (Nematoda).";
RL   PLoS ONE 5:E13343-E13343(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ISS 195;
RX   PubMed=21336279; DOI=10.1038/ng.769;
RA   Mitreva M., Jasmer D.P., Zarlenga D.S., Wang Z., Abubucker S., Martin J.,
RA   Taylor C.M., Yin Y., Fulton L., Minx P., Yang S.P., Warren W.C.,
RA   Fulton R.S., Bhonagiri V., Zhang X., Hallsworth-Pepin K., Clifton S.W.,
RA   McCarter J.P., Appleton J., Mardis E.R., Wilson R.K.;
RT   "The draft genome of the parasitic nematode Trichinella spiralis.";
RL   Nat. Genet. 43:228-235(2011).
CC   -!- FUNCTION: Binds iron and zinc. May bind nickel.
CC       {ECO:0000269|PubMed:20967224}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20967224}.
CC   -!- TISSUE SPECIFICITY: Expressed in larval tissues like cuticle,
CC       hypodermis and muscle (at protein level). Note=Not excreted into
CC       striated muscle fibers or nurse cell. {ECO:0000269|PubMed:20967224}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:20967224}.
CC   -!- MASS SPECTROMETRY: Mass=48105; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:20967224};
CC   -!- CAUTION: At protein level, the N-terminus was determined to be Leu-118
CC       but it is uncertain whether this is the true N-terminus of the mature
CC       protein or not. {ECO:0000305|PubMed:20967224}.
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DR   EMBL; GQ497342; ADD82426.1; -; mRNA.
DR   EMBL; ABIR02000995; EFV55596.1; -; Genomic_DNA.
DR   RefSeq; XP_003374855.1; XM_003374807.1.
DR   AlphaFoldDB; D4N4Z9; -.
DR   SMR; D4N4Z9; -.
DR   iPTMnet; D4N4Z9; -.
DR   EnsemblMetazoa; EFV55596; EFV55596; EFV55596.
DR   GeneID; 10904460; -.
DR   KEGG; tsp:Tsp_04041; -.
DR   CTD; 10904460; -.
DR   HOGENOM; CLU_647847_0_0_1; -.
DR   OMA; KCCCHPY; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; IDA:UniProtKB.
DR   GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Iron; Metal-binding; Secreted;
KW   Signal; Zinc.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..424
FT                   /note="Poly-cysteine and histidine-tailed protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000581037"
FT   REGION          372..424
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        372..390
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        291
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305|PubMed:20967224"
FT   CARBOHYD        397
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305|PubMed:20967224"
FT   CONFLICT        121
FT                   /note="I -> L (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   424 AA;  47739 MW;  7018E80DF2DE72AE CRC64;
     MAFSTIVVLF VAAVGFGNKI SSADTCPEFG EWKPWTECLW YPMQNIYDKM TASCGLPGHR
     NLTNILPLPP GFTIPPPCGH CSFKTRCRTR PKKEGCYPFD GEREICHEHG DICTIAKLPG
     IGCGWTVLQE VVKQCLSRPD IPEYMRAGYK KLFHMLPKGH CIEKDNQCKC CCGDYEPNED
     GTECVKQQDH QCAPFNEPGD WSECLWFPLA DMFKKVQSHC GVEGKPEGLS PSSLAPAGFQ
     IPEKCGFCSF RLKCQSREKK EGCFPLKVDK KSCGAEDCPT CGDVCTLDKQ NNSCAFTKAM
     GMKFWNSFAH KAKESNLAHW RRDGYADLFK FLPYGHCKEV GDKCKCCCHP YEPNEDGTAC
     VVKQYCKSLE EVGGKKQQKD QPESEKKAEN MPETTGNASH HQHRHHHGDS SSESHEQHHH
     HHHH