ID   PCKA_ASHGO              Reviewed;         541 AA.
AC   Q753M0;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
DE            EC=4.1.1.49;
GN   Name=PCK1; OrderedLocusNames=AFR292W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC         EC=4.1.1.49;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC       family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53663.1; -; Genomic_DNA.
DR   RefSeq; NP_985839.1; NM_211194.1.
DR   AlphaFoldDB; Q753M0; -.
DR   SMR; Q753M0; -.
DR   STRING; 33169.AAS53663; -.
DR   EnsemblFungi; AAS53663; AAS53663; AGOS_AFR292W.
DR   GeneID; 4622102; -.
DR   KEGG; ago:AGOS_AFR292W; -.
DR   eggNOG; ENOG502QQI5; Eukaryota.
DR   HOGENOM; CLU_018247_2_0_1; -.
DR   InParanoid; Q753M0; -.
DR   OMA; MRYAGEM; -.
DR   UniPathway; UPA00138; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IBA:GO_Central.
DR   GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR   CDD; cd00484; PEPCK_ATP; 1.
DR   Gene3D; 3.40.449.10; -; 1.
DR   Gene3D; 3.90.228.20; -; 1.
DR   HAMAP; MF_00453; PEPCK_ATP; 1.
DR   InterPro; IPR001272; PEP_carboxykinase_ATP.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   InterPro; IPR015994; PEPCK_ATP_CS.
DR   PANTHER; PTHR30031; PTHR30031; 1.
DR   Pfam; PF01293; PEPCK_ATP; 1.
DR   PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR   SUPFAM; SSF68923; SSF68923; 1.
DR   TIGRFAMs; TIGR00224; pckA; 1.
DR   PROSITE; PS00532; PEPCK_ATP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Decarboxylase; Gluconeogenesis; Lyase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..541
FT                   /note="Phosphoenolpyruvate carboxykinase (ATP)"
FT                   /id="PRO_0000203869"
FT   BINDING         243..250
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   541 AA;  59914 MW;  0FA86DAA21491092 CRC64;
     MSPSKVYQTP EEKIRAELGL SSGVLTIRRN APAAVLYEDA LAERKTAISS TGALIAYSGE
     KTGRSPKDKR IVDEPTSSDN IWWGPVNRKA SEKTWLINRE RAADFLRTRE HLYIVDAYAG
     WDPRYRIKIR IVCCRAYHAL FMTNMLIRPT EEELAEFGEP DFTVWNAGQF PANTHTEGMS
     SKTTVEINFR AMEMVILGTE YAGEMKKGIF TVMFYLMPIN HNVLTLHSSA NQGPNNDVTL
     FFGLSGTGKT TLSADQHRKL IGDDEHCWSD YGVFNIEGGC YAKCIGLSGE KEPEIFNAIK
     YGSVLENVVY DPVTRVVDYE DSSITENTRC AYPIEYIPSA QIPCLSENHP SNIVLLTCDA
     SGVLPPVSRL TPEQVMYHFI SGYTSKMAGT EQGVTEPEAT FSSCFGQPFL ALHPMKYATM
     LAEKMSQHNA SAWLINTGWT GSSYTAGGKR CPLKYTRAIL DSIHDGTLAQ AEYETLPVFG
     LSIPKAVEGV PAELLNPAKN WVEGEGKYAS AVSALASKFT ENFKIYQDQA TEEVVRAGPQ
     I