ID   PCKA_CUCSA              Reviewed;         670 AA.
AC   P42066;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
DE            Short=PEP carboxykinase;
DE            Short=PEPCK;
DE            EC=4.1.1.49;
GN   Name=PCK;
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Masterpiece; TISSUE=Cotyledon;
RX   PubMed=7948888; DOI=10.1007/bf00039551;
RA   Kim D.-J., Smith S.M.;
RT   "Molecular cloning of cucumber phosphoenolpyruvate carboxykinase and
RT   developmental regulation of gene expression.";
RL   Plant Mol. Biol. 26:423-434(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC         EC=4.1.1.49;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Cotyledons, leaves and roots.
CC   -!- DEVELOPMENTAL STAGE: It is synthesized a few days immediately following
CC       germination, and again a few weeks later when the cotyledons senesce.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC       family. {ECO:0000305}.
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DR   EMBL; L31899; AAA64739.1; -; mRNA.
DR   PIR; S52637; S52637.
DR   RefSeq; NP_001292717.1; NM_001305788.1.
DR   AlphaFoldDB; P42066; -.
DR   SMR; P42066; -.
DR   STRING; 3659.XP_004136241.1; -.
DR   GeneID; 101222382; -.
DR   KEGG; csv:101222382; -.
DR   eggNOG; ENOG502QQI5; Eukaryota.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   CDD; cd00484; PEPCK_ATP; 1.
DR   Gene3D; 3.40.449.10; -; 1.
DR   Gene3D; 3.90.228.20; -; 1.
DR   HAMAP; MF_00453; PEPCK_ATP; 1.
DR   InterPro; IPR001272; PEP_carboxykinase_ATP.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   InterPro; IPR015994; PEPCK_ATP_CS.
DR   PANTHER; PTHR30031; PTHR30031; 1.
DR   Pfam; PF01293; PEPCK_ATP; 1.
DR   SUPFAM; SSF68923; SSF68923; 1.
DR   TIGRFAMs; TIGR00224; pckA; 1.
DR   PROSITE; PS00532; PEPCK_ATP; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Decarboxylase; Gluconeogenesis; Lyase;
KW   Nucleotide-binding.
FT   CHAIN           1..670
FT                   /note="Phosphoenolpyruvate carboxykinase (ATP)"
FT                   /id="PRO_0000203863"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        23..48
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         368..375
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   670 AA;  74397 MW;  51033130B6E77DB6 CRC64;
     MENEGKDNGE FSFVSDGGAE TGRRGLPKIH TEKNAPTTER DICHDDSTTP MRARTLEHLH
     SLQKKRSTPT TPLTDSQGVF SPVSEAERQK QQLISISASL ASLTRETGPK LVKGDPEKKK
     EAHKASVLDH LHFGEPILNL SDSALKSTHI LYNPSPAELY EQAIKYEKGS FITSTGALAT
     LSGAKTGRSP IDKRVVKDDT TEKELWWGKG SPNIEMDEHT FLINRERAVD YLNSLDKVFV
     NDQFLNWDPE NRIKVRIVSA RAYHSLFMHN MCIRPTAGEL EDFGTPDFTI YHAGQFPCNR
     YTHYMTSSTS IDMNLDRKEM VILGTQYAGE MKKGLFSLMH YLMPMRQILS LHSGCNMGKN
     GDVALFFGLS GTGKTTLSTD HNRYLIGDDE HCWSDNGVSN IEGGCYAKCI DLSREKEPDI
     WNAIKFGTVL ENVVFDEHTR EVDYSEKSVT ENTRAAYPIE YIPNAKIPCV GPHPKNVILL
     ACDAFGVLPP VSKLSLPQTM YHFISGYTAL VAGTEEGVKE PQATFSACFG AAFIMLHPSR
     YAAMLAEKMK KHGATGWLVN TGWSGGSYGS GNRIKLAYTR KIIDAIHSGA LLEANYSKTR
     VFGLEIPDAI EGVPSHILDP INTWSDKDGY HETLLKLGGL FKKNYEGIHT YQVERDSELA
     EEILAAGPTL