ID   PCKA_DICDI              Reviewed;         562 AA.
AC   Q75JD5; Q55AN2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
DE            EC=4.1.1.49;
GN   Name=pckA; ORFNames=DDB_G0271678;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC         EC=4.1.1.49;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC       family. {ECO:0000305}.
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DR   EMBL; AAFI02000006; EAL71529.1; -; Genomic_DNA.
DR   RefSeq; XP_645490.1; XM_640398.1.
DR   AlphaFoldDB; Q75JD5; -.
DR   SMR; Q75JD5; -.
DR   STRING; 44689.DDB0231108; -.
DR   PaxDb; Q75JD5; -.
DR   EnsemblProtists; EAL71529; EAL71529; DDB_G0271678.
DR   GeneID; 8618118; -.
DR   KEGG; ddi:DDB_G0271678; -.
DR   dictyBase; DDB_G0271678; pckA.
DR   eggNOG; ENOG502QQI5; Eukaryota.
DR   HOGENOM; CLU_018247_0_1_1; -.
DR   InParanoid; Q75JD5; -.
DR   OMA; MRYAGEM; -.
DR   PhylomeDB; Q75JD5; -.
DR   UniPathway; UPA00138; -.
DR   PRO; PR:Q75JD5; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IBA:GO_Central.
DR   GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR   CDD; cd00484; PEPCK_ATP; 1.
DR   Gene3D; 3.40.449.10; -; 1.
DR   Gene3D; 3.90.228.20; -; 1.
DR   HAMAP; MF_00453; PEPCK_ATP; 1.
DR   InterPro; IPR001272; PEP_carboxykinase_ATP.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   PANTHER; PTHR30031; PTHR30031; 1.
DR   Pfam; PF01293; PEPCK_ATP; 1.
DR   PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR   SUPFAM; SSF68923; SSF68923; 1.
DR   TIGRFAMs; TIGR00224; pckA; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Decarboxylase; Gluconeogenesis; Lyase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..562
FT                   /note="Phosphoenolpyruvate carboxykinase (ATP)"
FT                   /id="PRO_0000327804"
FT   BINDING         265..272
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   562 AA;  62570 MW;  27C1020574CAB5CD CRC64;
     MNQAVAESIN KSLKSSTADY TPKGYHITEL ESDSIYLDEH FSGNAKIFHN PAVAVLYEQA
     LAFEHGSAIT STGALVTRSG VKTGRSPKDK RIVKEPSSQD DIWWGPVNIA MDDLSFMINR
     ERAIDYLNTQ EKIYVIDGYA GWDPKYRIKV RVICARAYHA LFMHNMLIRP ANREELRNFG
     EPDYTIYNAG QFPANRYTKG MSSSSSIAID FARKEMVILG TQYAGEMKKG ILTIMMYLMP
     KMGVLPLHSS CNQARNNGDT TLFFGLSGTG KTTLSADINR ELIGDDEHVW TDTGCFNIEG
     GCYAKCIDLS REKEPEIFDA IKFGAVLENV VYNEYSRKVD YNDVSITENT RCAYPLEHIP
     NAKFPAIAGH PKNIIMLTCD AFGILPPVSR LDANQVMYHF IQGYTAKVAG TEVGVTEPTA
     TFSSCYGEPF IVWHPTKYAE MLASQLHKHS ARAWLINTGW TGGSHGVGSR IKLAYTRAII
     DAIHSGELEK IPTTKMDVFG FQVPNSCPGV PSEILMPING WADKEKYVST MHKLAKLFIE
     NFKKFQDKAS PELVAAGPIL PQ