ID   PCKA_EMENI              Reviewed;         600 AA.
AC   Q96UL8; C8VKU0; Q5BC12;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 3.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
DE            EC=4.1.1.49;
GN   Name=acuF; ORFNames=AN1918;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11741859; DOI=10.1128/jb.184.1.183-190.2002;
RA   Hynes M.J., Draht O.W., Davis M.A.;
RT   "Regulation of the acuF gene, encoding phosphoenolpyruvate carboxykinase in
RT   the filamentous fungus Aspergillus nidulans.";
RL   J. Bacteriol. 184:183-190(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC         EC=4.1.1.49;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL10705.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=EAA65083.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY049067; AAL10705.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AACD01000029; EAA65083.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BN001307; CBF85824.1; -; Genomic_DNA.
DR   RefSeq; XP_659522.1; XM_654430.1.
DR   AlphaFoldDB; Q96UL8; -.
DR   SMR; Q96UL8; -.
DR   STRING; 162425.CADANIAP00008578; -.
DR   PRIDE; Q96UL8; -.
DR   EnsemblFungi; CBF85824; CBF85824; ANIA_01918.
DR   EnsemblFungi; EAA65083; EAA65083; AN1918.2.
DR   GeneID; 2875509; -.
DR   KEGG; ani:AN1918.2; -.
DR   VEuPathDB; FungiDB:AN1918; -.
DR   eggNOG; ENOG502QQI5; Eukaryota.
DR   HOGENOM; CLU_018247_2_0_1; -.
DR   InParanoid; Q96UL8; -.
DR   OMA; MRYAGEM; -.
DR   OrthoDB; 380257at2759; -.
DR   UniPathway; UPA00138; -.
DR   Proteomes; UP000000560; Chromosome VII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IBA:GO_Central.
DR   GO; GO:0006083; P:acetate metabolic process; IEP:AspGD.
DR   GO; GO:0015976; P:carbon utilization; IMP:AspGD.
DR   GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR   CDD; cd00484; PEPCK_ATP; 1.
DR   Gene3D; 3.40.449.10; -; 1.
DR   Gene3D; 3.90.228.20; -; 1.
DR   HAMAP; MF_00453; PEPCK_ATP; 1.
DR   InterPro; IPR001272; PEP_carboxykinase_ATP.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   InterPro; IPR015994; PEPCK_ATP_CS.
DR   PANTHER; PTHR30031; PTHR30031; 1.
DR   Pfam; PF01293; PEPCK_ATP; 1.
DR   PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR   SUPFAM; SSF68923; SSF68923; 1.
DR   TIGRFAMs; TIGR00224; pckA; 1.
DR   PROSITE; PS00532; PEPCK_ATP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Decarboxylase; Gluconeogenesis; Lyase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..600
FT                   /note="Phosphoenolpyruvate carboxykinase (ATP)"
FT                   /id="PRO_0000203872"
FT   BINDING         302..309
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        196
FT                   /note="A -> V (in Ref. 1; AAL10705)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        479
FT                   /note="M -> I (in Ref. 1; AAL10705)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   600 AA;  66430 MW;  671518BB53A82C9C CRC64;
     MAPGANIHIP PAGPPEPGPL YSDFYQQQIE RQRNNNYHST SLRNMVATSV NRTALHPGGV
     QPGKGHTELE EELHEHAHID YDRVAIIANP SVAALYEDAL VYETGTAITS SGALTAYSGA
     KTGRSPSDKR IVKEESSEKE VWWGPVNKPM TPDVWRINRE RAVDYLNTRN RIYVIDGFAG
     WDERYRISVR VVCARAYHAL FMRNMLIRPS AEELKHFHPD YVIYNAGSFP ANRFTEGMTS
     ATSVAINFAE KEMVILGTEY AGEMKKGVFT ILFYEMPVKH NVLTLHSSAN EGQNGDVTVF
     FGLSGTGKTT LSADPKRALI GDDEHCWTDR GVFNIEGGCY AKCIGLSAEK EPDIFNAIRF
     GSVLENVVFD PISRVVDYDD STLTENTRCA YPIEYIENAK VPCLSDSHPS NIILLTCDAR
     GVLPPISKLT TEQTMFHFIS GYTSKMAGTE DGVTEPQATF SSCFAQPFLA LHPMRYARML
     ADKISQHKAN AWLLNTGWVG AGATTGGKRC PLKYTRAILD AIHSGELAKA EYETYDVFNL
     HVPKSCPGVP DELLNPKNSW TATTSFSDEV NKLAKLFNEN FQKYADQATK EVIAAGPVVQ