PCKA_KLULA
ID PCKA_KLULA Reviewed; 543 AA.
AC O43112; Q6CYH0;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
DE EC=4.1.1.49;
GN Name=PCK1; OrderedLocusNames=KLLA0A00484g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=9717242;
RX DOI=10.1002/(sici)1097-0061(199807)14:10<963::aid-yea282>3.0.co;2-x;
RA Kitamoto H.K., Ohmomo S., Iimura Y.;
RT "Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate
RT carboxykinase from Kluyveromyces lactis.";
RL Yeast 14:963-967(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=4.1.1.49;
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC family. {ECO:0000305}.
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DR EMBL; U88575; AAC27661.1; -; Genomic_DNA.
DR EMBL; CR382121; CAH02607.1; -; Genomic_DNA.
DR RefSeq; XP_451019.1; XM_451019.1.
DR AlphaFoldDB; O43112; -.
DR SMR; O43112; -.
DR STRING; 28985.XP_451019.1; -.
DR EnsemblFungi; CAH02607; CAH02607; KLLA0_A00484g.
DR GeneID; 2896341; -.
DR KEGG; kla:KLLA0_A00484g; -.
DR eggNOG; ENOG502QQI5; Eukaryota.
DR HOGENOM; CLU_018247_0_1_1; -.
DR InParanoid; O43112; -.
DR OMA; MRYAGEM; -.
DR UniPathway; UPA00138; -.
DR Proteomes; UP000000598; Chromosome A.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR CDD; cd00484; PEPCK_ATP; 1.
DR Gene3D; 3.40.449.10; -; 1.
DR Gene3D; 3.90.228.20; -; 1.
DR HAMAP; MF_00453; PEPCK_ATP; 1.
DR InterPro; IPR001272; PEP_carboxykinase_ATP.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR InterPro; IPR015994; PEPCK_ATP_CS.
DR PANTHER; PTHR30031; PTHR30031; 1.
DR Pfam; PF01293; PEPCK_ATP; 1.
DR PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR SUPFAM; SSF68923; SSF68923; 1.
DR TIGRFAMs; TIGR00224; pckA; 1.
DR PROSITE; PS00532; PEPCK_ATP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Decarboxylase; Gluconeogenesis; Lyase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..543
FT /note="Phosphoenolpyruvate carboxykinase (ATP)"
FT /id="PRO_0000203873"
FT BINDING 244..251
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 236
FT /note="D -> G (in Ref. 1; AAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="A -> G (in Ref. 1; AAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 500
FT /note="S -> I (in Ref. 1; AAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 509
FT /note="K -> N (in Ref. 1; AAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 520
FT /note="L -> W (in Ref. 1; AAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 523
FT /note="E -> K (in Ref. 1; AAC27661)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 543 AA; 60429 MW; 35CD60147227B2A4 CRC64;
MSPTKTQNSA EENIRAELGL SAEVVTIRRN APAALLYEDA LKERDTAISN AGALIAYSGD
KTGRSPRDKR IVEEETSKDN VWWGPVNKPC SERTWEINRE RAADYLRTRD HIYIVDAYAG
WDPRYRIKVR VVCARAYHAL FMTNMLIRPS KEELENFGEP DFTVWNAGQF PANTHTSGMT
SKTTVEINFK QMEMVILGTE YAGEMKKGIF TVMFYLMPVN HNVLTLHSSA NQGIQDNDVT
LFFGLSGTGK TTLSADPHRL LIGDDEHCWS DHGVFNIEGG CYAKCLGLSA EKEPEIFNAI
KFGSVLENII YDPNTREVDY EDSTITENTR CAYPIEYIPS AKIPCLADHH PKNIVLLTCD
ASGVLPPVSK LTPDQVMYHF ISGYTSKMAG TEQGVTEPEA TFSSCFGQPF LSLHPMKYAT
MLAEKMAEHN ANAWLINTGW TGSSYVAGGK RCPLKYTRAI LDAIHDGSLA KEEYEVLPIF
NLQIPKAVGD KVPASLLNPS KNWAEGEAKY TSNVKSLANL FVENFKTYQD KATEQVLAAG
PQL