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ASPG1_DROME
ID   ASPG1_DROME             Reviewed;         393 AA.
AC   Q8MR45; A1Z7W5; A1Z7W6; Q6NN89;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase CG1827;
DE            EC=3.5.1.26;
DE   AltName: Full=Aspartylglucosaminidase {ECO:0000250|UniProtKB:P20933};
DE            Short=AGA {ECO:0000250|UniProtKB:P20933};
DE   AltName: Full=Glycosylasparaginase {ECO:0000250|UniProtKB:P20933};
DE   AltName: Full=N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase {ECO:0000250|UniProtKB:P20933};
DE   Contains:
DE     RecName: Full=Glycosylasparaginase alpha chain {ECO:0000250|UniProtKB:P20933};
DE   Contains:
DE     RecName: Full=Glycosylasparaginase beta chain {ECO:0000250|UniProtKB:P20933};
DE   Flags: Precursor;
GN   ORFNames=CG1827;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1] {ECO:0000312|EMBL:AAF58918.5}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:AAF58918.5}
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3] {ECO:0000305, ECO:0000312|EMBL:AAM52645.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley {ECO:0000312|EMBL:AAM52645.1};
RC   TISSUE=Head {ECO:0000269|PubMed:12537569};
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4] {ECO:0000305, ECO:0000312|EMBL:AAR96196.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Testis;
RA   Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA   Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to
CC       the peptide of asparagine-linked glycoproteins.
CC       {ECO:0000250|UniProtKB:P20933}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine = H(+)
CC         + L-aspartate + N-acetyl-beta-D-glucosaminylamine;
CC         Xref=Rhea:RHEA:11544, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15947, ChEBI:CHEBI:29991, ChEBI:CHEBI:58080; EC=3.5.1.26;
CC         Evidence={ECO:0000250|UniProtKB:P20933};
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta chains arranged as a
CC       dimer of alpha/beta heterodimers. {ECO:0000250|UniProtKB:P20933}.
CC   -!- PTM: Cleaved into an alpha and beta chain by autocatalysis; this
CC       activates the enzyme. The N-terminal residue of the beta subunit is
CC       responsible for the nucleophile hydrolase activity (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Ntn-hydrolase family. {ECO:0000255}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAR96196.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR   EMBL; AE013599; AAF58918.5; -; Genomic_DNA.
DR   EMBL; AY122133; AAM52645.1; -; mRNA.
DR   EMBL; BT011404; AAR96196.1; ALT_SEQ; mRNA.
DR   RefSeq; NP_610504.4; NM_136660.3.
DR   AlphaFoldDB; Q8MR45; -.
DR   SMR; Q8MR45; -.
DR   STRING; 7227.FBpp0271751; -.
DR   MEROPS; T02.001; -.
DR   GlyGen; Q8MR45; 2 sites.
DR   PaxDb; Q8MR45; -.
DR   PRIDE; Q8MR45; -.
DR   DNASU; 35989; -.
DR   EnsemblMetazoa; FBtr0339392; FBpp0308484; FBgn0033431.
DR   GeneID; 35989; -.
DR   KEGG; dme:Dmel_CG1827; -.
DR   UCSC; CG1827-RB; d. melanogaster.
DR   UCSC; CG1827-RC; d. melanogaster.
DR   FlyBase; FBgn0033431; CG1827.
DR   VEuPathDB; VectorBase:FBgn0033431; -.
DR   eggNOG; KOG1593; Eukaryota.
DR   GeneTree; ENSGT00950000183045; -.
DR   HOGENOM; CLU_021603_0_0_1; -.
DR   InParanoid; Q8MR45; -.
DR   OMA; PDENCET; -.
DR   OrthoDB; 1487354at2759; -.
DR   PhylomeDB; Q8MR45; -.
DR   Reactome; R-DME-6798695; Neutrophil degranulation.
DR   BioGRID-ORCS; 35989; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 35989; -.
DR   PRO; PR:Q8MR45; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0033431; Expressed in seminal fluid secreting gland and 24 other tissues.
DR   Genevisible; Q8MR45; DM.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0003948; F:N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; ISS:UniProtKB.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006517; P:protein deglycosylation; ISS:UniProtKB.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR000246; Peptidase_T2.
DR   PANTHER; PTHR10188; PTHR10188; 1.
DR   Pfam; PF01112; Asparaginase_2; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
PE   2: Evidence at transcript level;
KW   Autocatalytic cleavage; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW   Reference proteome; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..242
FT                   /note="Glycosylasparaginase alpha chain"
FT                   /evidence="ECO:0000250|UniProtKB:P20933"
FT                   /id="PRO_0000384129"
FT   CHAIN           243..393
FT                   /note="Glycosylasparaginase beta chain"
FT                   /evidence="ECO:0000250|UniProtKB:P20933"
FT                   /id="PRO_0000384130"
FT   ACT_SITE        243
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:P20933"
FT   BINDING         271..274
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         294..297
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        49
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        64
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        97..102
FT                   /evidence="ECO:0000250|UniProtKB:P20933"
FT   DISULFID        196..212
FT                   /evidence="ECO:0000250|UniProtKB:P20933"
FT   DISULFID        354..381
FT                   /evidence="ECO:0000250|UniProtKB:P20933"
FT   CONFLICT        370
FT                   /note="A -> G (in Ref. 4; AAR96196)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   393 AA;  42240 MW;  993979A39BF9129C CRC64;
     MRRHLRASLW ILCLATMAFS ILAAVNTSPK PTLTSAFSGK AGTTAVKANK TTGELLPMVI
     NTWNFTAANV LAWRILKQSK GGLRQTRNAV VEGCSKCEKL QCDRTVGYGG SPDELGETTL
     DAMVMDGATM DVGAVAGLRR IKDAIKVARH VLEHTQHTML VGDAASAFAN AMGFESESLV
     TPESKDMWLQ WTAENCQPNF WKNVHPDPKV SCGPYKPRPT PLTRWKEDRA RNEYEIGRKN
     HDTIGMIAID VESNIHAGTS TNGARHKIPG RVGDSPIPGA GAYADNEVGA AVATGDGDVM
     MRFLPSLLAV ETMRAGKPPA EAAQEGLRRI LKHHKDFMGA LIAVDRLGNY GAACYGLAEF
     PFMVSSPAGA DGPTRLETVK CIGGQDKVNI VAL
 
 
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