ID   PCKA_TRYBB              Reviewed;         472 AA.
AC   P13735;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase (ATP), glycosomal;
DE            EC=4.1.1.49;
DE   AltName: Full=Glycosomal protein p60;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2925689; DOI=10.1016/s0021-9258(18)83719-x;
RA   Kueng V., Schlaeppi K., Schneider A., Seebeck T.;
RT   "A glycosomal protein (p60) which is predominantly expressed in procyclic
RT   Trypanosoma brucei. Characterization and DNA sequence.";
RL   J. Biol. Chem. 264:5203-5209(1989).
RN   [2]
RP   FUNCTION.
RX   PubMed=2671940; DOI=10.1093/nar/17.15.6411;
RA   Parsons M., Smith J.M.;
RT   "Trypanosome glycosomal protein P60 is homologous to phosphoenolpyruvate
RT   carboxykinase (ATP).";
RL   Nucleic Acids Res. 17:6411-6411(1989).
CC   -!- FUNCTION: P60 has the capability to bind to microtubules and membrane
CC       vesicles in vitro. {ECO:0000269|PubMed:2671940}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC         EC=4.1.1.49;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Glycosome.
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC       family. {ECO:0000305}.
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DR   EMBL; M20570; AAA30199.1; -; Genomic_DNA.
DR   PIR; A33275; A33275.
DR   AlphaFoldDB; P13735; -.
DR   SMR; P13735; -.
DR   PRIDE; P13735; -.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0020015; C:glycosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.449.10; -; 1.
DR   Gene3D; 3.90.228.20; -; 1.
DR   HAMAP; MF_00453; PEPCK_ATP; 1.
DR   InterPro; IPR001272; PEP_carboxykinase_ATP.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   InterPro; IPR015994; PEPCK_ATP_CS.
DR   PANTHER; PTHR30031; PTHR30031; 1.
DR   Pfam; PF01293; PEPCK_ATP; 1.
DR   PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR   SUPFAM; SSF68923; SSF68923; 1.
DR   PROSITE; PS00532; PEPCK_ATP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Decarboxylase; Gluconeogenesis; Glycosome; Lyase;
KW   Nucleotide-binding; Peroxisome.
FT   CHAIN           1..472
FT                   /note="Phosphoenolpyruvate carboxykinase (ATP), glycosomal"
FT                   /id="PRO_0000203867"
FT   BINDING         221..228
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   472 AA;  52457 MW;  82768661A5A04786 CRC64;
     MAPIIHKNLT APELVEWALK LEKDSQLTAR GALSVRSYAK TGRSPRDKRI VNTTDVTDNV
     DWGSVNMKLT EESFEKLKTI AKDYFATCKH LFVMDCFAGH DERYRLKVRV YTTRPYHALF
     MRNMLIVPTL EELQSFGEPD YVIYNAGEAK ADPTVPGVTS TTSVALNFKT REQVILGTEY
     AGEMKKGILT VMFELMPRMG HLCMHASANV GKSGDVTVFF GLSGTGKTTL SADPRRNLIG
     DDEHVWTDRG VFNIEGGCYA KAIGLNPETE KDIYEAVRFG AVAENCTLDR RTHEIDFNDE
     SICKNTRVAY PLMHIDGALS KAVAGHPKNI IFLTNDAFGV MPPVARLTSA QAMFWFVMGY
     TANVPGVEAG SARVARPIFS SCFGGPFLVR HATHYGQQLA EKMEKHNSRV WLLNTGYAGG
     RADRGAKRMP LRVTRAIIDA IHDGTLDQAD YEVYPGWAFT FLRGLRTCPQ AC