PCKA_YEAST
ID PCKA_YEAST Reviewed; 549 AA.
AC P10963; D6VXF8; E9P963;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 03-AUG-2022, entry version 189.
DE RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
DE EC=4.1.1.49;
GN Name=PCK1; Synonyms=PEPC, PPC1; OrderedLocusNames=YKR097W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3060853; DOI=10.1093/nar/16.22.10926;
RA Stucka R., Veldes-Hevia K.D., Gancedo C., Feldmann H.;
RT "Nucleotide sequence of the phosphoenolpyruvate carboxykinase gene from
RT Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 16:10926-10926(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7756267; DOI=10.1021/bi00019a017;
RA Krautwurst H., Encinas M.V., Marcus F., Latshaw S.P., Kemp R.G., Frey P.A.,
RA Cardemil E.;
RT "Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: revised amino
RT acid sequence, site-directed mutagenesis, and microenvironment
RT characteristics of cysteines 365 and 458.";
RL Biochemistry 34:6382-6388(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP PROTEIN SEQUENCE OF 359-376.
RX PubMed=1540632; DOI=10.1016/0167-4838(92)90230-b;
RA Alvear M., Encinas M.V., Kemp R.G., Latshaw S.P., Cardemil E.;
RT "ATP-dependent Saccharomyces cerevisiae phospho enol pyruvate
RT carboxykinase: isolation and sequence of a peptide containing a highly
RT reactive cysteine.";
RL Biochim. Biophys. Acta 1119:35-38(1992).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=4.1.1.49;
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC -!- SUBUNIT: Homotetramer.
CC -!- INTERACTION:
CC P10963; P33203: PRP40; NbExp=2; IntAct=EBI-13770, EBI-701;
CC P10963; P39940: RSP5; NbExp=2; IntAct=EBI-13770, EBI-16219;
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC family. {ECO:0000305}.
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DR EMBL; X13096; CAA31488.1; -; Genomic_DNA.
DR EMBL; U24234; AAA76693.1; -; mRNA.
DR EMBL; Z28322; CAA82177.1; -; Genomic_DNA.
DR EMBL; AY723843; AAU09760.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09248.1; -; Genomic_DNA.
DR PIR; A56461; A56461.
DR RefSeq; NP_013023.3; NM_001179887.3.
DR AlphaFoldDB; P10963; -.
DR SMR; P10963; -.
DR BioGRID; 34228; 119.
DR IntAct; P10963; 8.
DR MINT; P10963; -.
DR STRING; 4932.YKR097W; -.
DR MaxQB; P10963; -.
DR PaxDb; P10963; -.
DR PRIDE; P10963; -.
DR EnsemblFungi; YKR097W_mRNA; YKR097W; YKR097W.
DR GeneID; 853972; -.
DR KEGG; sce:YKR097W; -.
DR SGD; S000001805; PCK1.
DR VEuPathDB; FungiDB:YKR097W; -.
DR eggNOG; ENOG502QQI5; Eukaryota.
DR HOGENOM; CLU_018247_0_1_1; -.
DR InParanoid; P10963; -.
DR OMA; MRYAGEM; -.
DR BioCyc; YEAST:YKR097W-MON; -.
DR BRENDA; 4.1.1.32; 984.
DR BRENDA; 4.1.1.49; 984.
DR SABIO-RK; P10963; -.
DR UniPathway; UPA00138; -.
DR PRO; PR:P10963; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P10963; protein.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IDA:SGD.
DR GO; GO:0006094; P:gluconeogenesis; IEP:SGD.
DR CDD; cd00484; PEPCK_ATP; 1.
DR Gene3D; 3.40.449.10; -; 1.
DR Gene3D; 3.90.228.20; -; 1.
DR HAMAP; MF_00453; PEPCK_ATP; 1.
DR InterPro; IPR001272; PEP_carboxykinase_ATP.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR InterPro; IPR015994; PEPCK_ATP_CS.
DR PANTHER; PTHR30031; PTHR30031; 1.
DR Pfam; PF01293; PEPCK_ATP; 1.
DR PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR SUPFAM; SSF68923; SSF68923; 1.
DR TIGRFAMs; TIGR00224; pckA; 1.
DR PROSITE; PS00532; PEPCK_ATP; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Decarboxylase; Direct protein sequencing; Gluconeogenesis;
KW Lyase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..549
FT /note="Phosphoenolpyruvate carboxykinase (ATP)"
FT /id="PRO_0000203875"
FT BINDING 250..257
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 95..107
FT /note="PCSERTWSINRER -> HVLKEHGLSTVK (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="A -> R (in Ref. 1; CAA31488)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="H -> Q (in Ref. 1; CAA31488)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="P -> L (in Ref. 5; AAU09760)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="K -> N (in Ref. 2; AAA76693)"
FT /evidence="ECO:0000305"
FT CONFLICT 544..549
FT /note="AGPQFE -> DWSSIRVNETC (in Ref. 1; CAA31488)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 549 AA; 60983 MW; C1A3669075052EC0 CRC64;
MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN KTVISSSGAL
IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT WSINRERAAD YLRTRDHIYI
VDAFAGWDPK YRIKVRVVCA RAYHALFMTN MLIRPTEEEL AHFGEPDFTV WNAGQFPANL
HTQDMSSKST IEINFKAMEM IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI
QNGDVTLFFG LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP
EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP CLADSHPKNI
ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG VTEPEPTFSS CFGQPFLALH
PIRYATMLAT KMSQHKANAY LINTGWTGSS YVSGGKRCPL KYTRAILDSI HDGSLANETY
ETLPIFNLQV PTKVNGVPAE LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD
VLAAGPQFE
